Detailed view for TcCLB.510259.18

Basic information

TDR Targets ID: 43271
Trypanosoma cruzi, GlcNAc-PI synthesis protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8072 | Length (AA): 455 | MW (Da): 51657 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00534   Glycosyl transferases group 1
PF08288   PIGA (GPI anchor biosynthesis)

Gene Ontology

Mouse over links to read term descriptions.
GO:0009058   biosynthetic process  
GO:0006506   GPI anchor biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 388 1v4v (A) 4 371 13.00 0 1 1 -0.87
4 385 2bis (A) 1 432 21.00 0 1 1.09 -0.88
183 374 2bfw (A) 218 412 22.00 0 1 0.69 -1.45
2 386 3c48 (A) 0 410 20.00 0 1 1.13705 -0.38
208 360 3qhp (A) 202 352 14.00 0.0000029 0.99 0.646164 -1.18
253 378 4rbn (A) 616 757 32.00 0.00018 0.95 0.512223 0.52
407 453 1lqs (L) 59 103 42.00 0.27 0.19 0.488597 0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127105)

Species Accession Gene Product
Arabidopsis thaliana AT3G45100   glycosylphosphatidylinositol-N-acetylglucosaminyltransferase subunit PIG-A-like protein SETH
Babesia bovis BBOV_III008560   glycosyl transferase, group 1 family protein
Brugia malayi Bm1_42330   N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Candida albicans CaO19.8117   glycosyl-phosphatidylinositol synthesis
Candida albicans CaO19.487   glycosyl-phosphatidylinositol synthesis
Caenorhabditis elegans CELE_D2085.6   Protein PIGA-1
Cryptosporidium hominis Chro.50057   glycan synthetase
Cryptosporidium hominis Chro.40236   N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Cryptosporidium parvum cgd5_3140   LPS glycosyltransferase of possible cyanobacterial origin
Cryptosporidium parvum cgd4_2100   PIG-A like N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Dictyostelium discoideum DDB_G0283965   GlcNAc transferase
Drosophila melanogaster Dmel_CG6401   CG6401 gene product from transcript CG6401-RB
Echinococcus granulosus EgrG_002062100   phosphatidylinositol
Echinococcus granulosus EgrG_002006650   glycosyltransferase
Entamoeba histolytica EHI_105870   phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
Echinococcus multilocularis EmuJ_000335800   phosphatidylinositol
Giardia lamblia GL50803_113610   GlcNAc-PI synthesis protein
Homo sapiens ENSG00000165195   phosphatidylinositol glycan anchor biosynthesis, class A
Leishmania braziliensis LbrM.33.1940   UDP-GlcNAc:PI a1-6 GlcNAc-transferase
Leishmania donovani LdBPK_331770.1   GlcNAc-PI synthesis protein
Leishmania infantum LinJ.33.1770   UDP-GlcNAc:PI a1-6 GlcNAc-transferase
Leishmania major LmjF.33.1670   UDP-GlcNAc:PI a1-6 GlcNAc-transferase
Leishmania mexicana LmxM.32.1670   N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein, putative
Loa Loa (eye worm) LOAG_00914   hypothetical protein
Mycobacterium leprae ML1715   POSSIBLE TRANSFERASE
Mus musculus ENSMUSG00000031381   phosphatidylinositol glycan anchor biosynthesis, class A
Mycobacterium tuberculosis Rv3032   Alpha (1-4) glucosyltransferase
Mycobacterium ulcerans MUL_1919   transferase
Mycobacterium ulcerans MUL_4676   glycosyltransferase
Neospora caninum NCLIV_017280   phosphatidylinositolglycan class A protein, putative
Plasmodium berghei PBANKA_0516200   phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
Plasmodium falciparum PF3D7_1032400   phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
Plasmodium knowlesi PKNH_0617200   phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
Plasmodium vivax PVX_111105   phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
Plasmodium yoelii PY03143   Arabidopsis thaliana At3g45100/T14D3_40-related
Saccharomyces cerevisiae YPL175W   Spt14p
Schistosoma japonicum Sjp_0117970   expressed protein
Schistosoma japonicum Sjp_0052190   ko:K03857 phosphatidylinositol glycan, class A, putative
Schistosoma mansoni Smp_046880.1   glycosyltransferase
Schistosoma mansoni Smp_046880.2   glycosyltransferase
Schmidtea mediterranea mk4.019512.01   Phosphatidylinositol N-acetylglucosaminyltransferase subunit A
Schmidtea mediterranea mk4.012220.00   Phosphatidylinositol N-acetylglucosaminyltransferase subunit A
Trypanosoma brucei gambiense Tbg.972.2.330   N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein, putative
Trypanosoma brucei Tb927.2.1780   GlcNAc-PI synthesis protein
Trypanosoma brucei Tb11.v5.0765   N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein, putative
Trypanosoma congolense TcIL3000_2_20   GlcNAc-PI synthesis protein
Trypanosoma cruzi TcCLB.510259.18   GlcNAc-PI synthesis protein
Trypanosoma cruzi TcCLB.509215.16   GlcNAc-PI synthesis protein
Toxoplasma gondii TGME49_241860   N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein PigA, family GT4 protein
Theileria parva TP04_0759   N-acetylglucosaminyl-phosphatidylinositol, putative

Essentiality

TcCLB.510259.18 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.1780 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.2.1780 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.1780 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.1780 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YPL175W Saccharomyces cerevisiae inviable yeastgenome
TGME49_241860 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.510259.18 (Trypanosoma cruzi), GlcNAc-PI synthesis protein
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