Detailed view for LbrM.21.1390

Basic information

TDR Targets ID: 434418
Leishmania braziliensis, PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Source Database / ID: 

pI: 7.882 | Length (AA): 788 | MW (Da): 84854 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05970   PIF1-like helicase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003678   DNA helicase activity  
GO:0006281   DNA repair  
GO:0000723   telomere maintenance  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
250 293 5fhh (A) 208 251 32.00 0 0.71 0.416567 -0.01
251 707 5fhh (A) 208 611 43.00 0 1 0.845814 0.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128192)

Species Accession Gene Product
Candida albicans CaO19.6133   DNA helicase
Candida albicans CaO19.13552   similar to putative DNA helicase involved in repair and recombination of mitochondrial DNA that also plays a role in (nuclear) c
Candida albicans CaO19.7538   similar to PIF1 DNA helicase
Caenorhabditis elegans CELE_T19C9.10   Protein T19C9.10, isoform A
Caenorhabditis elegans CELE_Y18H1A.6   Protein PIF-1
Dictyostelium discoideum DDB_G0268430   hypothetical protein
Dictyostelium discoideum DDB_G0277925   DNA helicase
Dictyostelium discoideum DDB_G0293212   hypothetical protein
Drosophila melanogaster Dmel_CG3238   CG3238 gene product from transcript CG3238-RB
Echinococcus granulosus EgrG_000792600   ATP dependent DNA helicase PIF1
Entamoeba histolytica EHI_014050   hypothetical protein, conserved
Entamoeba histolytica EHI_192830   DNA repair and recombination protein, putative
Echinococcus multilocularis EmuJ_000792600   ATP dependent DNA helicase PIF1
Giardia lamblia GL50803_16747   Rrm3p helicase
Homo sapiens ENSG00000140451   PIF1 5'-to-3' DNA helicase
Leishmania braziliensis LbrM.07.1070   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania braziliensis LbrM.21.1390   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania donovani LdBPK_211430.1   PIF1 helicase-like protein, putative
Leishmania donovani LdBPK_071170.1   PIF1 helicase-like protein, putative
Leishmania infantum LinJ.07.1170   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania infantum LinJ.21.1430   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania major LmjF.21.1190   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania major LmjF.07.1020   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania mexicana LmxM.07.1020   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Leishmania mexicana LmxM.21.1190   PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
Mus musculus ENSMUSG00000041064   PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae)
Saccharomyces cerevisiae YHR031C   Rrm3p
Schistosoma japonicum Sjp_0300680   ko:K01529 PIF1 5'-to-3' DNA helicase [EC:3.6.1.-], putative
Schistosoma mansoni Smp_147230   hypothetical protein
Schmidtea mediterranea mk4.000458.04   ATP-dependent DNA helicase PIF1
Trypanosoma brucei gambiense Tbg972.8.310   DNA repair and recombination helicase protein PIF1, putative,DNA repair and recombination protein, mitochondrial precursor, puta
Trypanosoma brucei gambiense Tbg972.10.1000   DNA repair and recombination helicase protein PIF1, putative,DNA repair and recombination protein, mitochondrial precursor, puta
Trypanosoma brucei Tb927.8.700   DNA repair and recombination helicase protein PIF7
Trypanosoma brucei Tb927.10.910   DNA repair and recombination helicase protein PIF6
Trypanosoma congolense TcIL3000_8_250   DNA repair and recombination protein, mitochondrial precursor, putative
Trypanosoma congolense TcIL3000_10_770   DNA repair and recombination helicase protein PIF6
Trypanosoma cruzi TcCLB.506855.280   DNA repair and recombination helicase protein PIF6, putative
Trypanosoma cruzi TcCLB.508567.80   DNA repair and recombination helicase protein PIF7, putative
Trypanosoma cruzi TcCLB.509611.10   DNA repair and recombination helicase protein PIF7, putative
Trichomonas vaginalis TVAG_352740   conserved hypothetical protein

Essentiality

LbrM.21.1390 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.910 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.910 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.700 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.700 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.700 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.700 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Hepatitis C virus Hepatitis C virus NS5B RNA-dependent RNA polymerase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LbrM.21.1390 (Leishmania braziliensis), PIF1 helicase-like protein, putative,DNA repair and recombination protein, mitochondrial precursor, putative
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