Detailed view for LinJ.32.0920

Basic information

TDR Targets ID: 440449
Leishmania infantum, phenylalanyl-tRNA synthetase alpha chain, putative
Source Database / ID: 

pI: 7.6436 | Length (AA): 499 | MW (Da): 56726 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01409   tRNA synthetases class II core domain (F)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004826   phenylalanine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0000049   tRNA binding  
GO:0043039   tRNA aminoacylation  
GO:0006432   phenylalanyl-tRNA aminoacylation  

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 498 3l4g (C) 1 494 45.00 0 1 1.4333 0.68
1 52 2p5v (A) 4 55 15.00 0.12 0.09 0.327508 -0.82
4 62 4kmf (A) 5 63 19.00 0.064 0.36 0.462536 -1.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127036)

Species Accession Gene Product
Arabidopsis thaliana AT4G39280   probable phenylalanine--tRNA ligase alpha subunit
Babesia bovis BBOV_III007240   phenylalanyl-tRNA synthetase family protein
Brugia malayi Bm1_08535   phenylalanyl (f) trna synthetase protein 1
Candida albicans CaO19.10477   phenylalanyl-tRNA synthetase
Candida albicans CaO19.2960   phenylalanyl-tRNA synthetase
Caenorhabditis elegans CELE_T08B2.9   Protein FARS-1, isoform A
Cryptosporidium hominis Chro.30378   phenylalanyl-trna synthetase-like protein
Cryptosporidium parvum cgd3_3320   putative phenylalanyl-tRNA synthetase
Chlamydia trachomatis CT_836   phenylalanine--tRNA ligase subunit alpha
Dictyostelium discoideum DDB_G0287141   phenylalanine-tRNA ligase, alpha subunit
Drosophila melanogaster Dmel_CG2263   CG2263 gene product from transcript CG2263-RA
Escherichia coli b1714   phenylalanine tRNA synthetase, alpha subunit
Echinococcus granulosus EgrG_001147900   phenylalanyl tRNA synthetase alpha chain B
Entamoeba histolytica EHI_078230   phenylalanyl-tRNA synthetase alpha subunit, putative
Echinococcus multilocularis EmuJ_001147900   phenylalanyl tRNA synthetase alpha chain B
Giardia lamblia GL50803_12162   Phenylalanyl-tRNA synthetase alpha chain
Homo sapiens ENSG00000179115   phenylalanyl-tRNA synthetase, alpha subunit
Leishmania braziliensis LbrM.32.0960   phenylalanyl-tRNA synthetase alpha chain, putative
Leishmania donovani LdBPK_320920.1   phenylalanyl-tRNA synthetase alpha chain, putative
Leishmania infantum LinJ.32.0920   phenylalanyl-tRNA synthetase alpha chain, putative
Leishmania major LmjF.32.0870   phenylalanyl-tRNA synthetase alpha chain, putative
Leishmania mexicana LmxM.31.0870   phenylalanyl-tRNA synthetase alpha chain, putative
Loa Loa (eye worm) LOAG_01536   hypothetical protein
Mycobacterium leprae ML1401   Probable phenylalanyl-tRNA synthetase, alpha chain PheS
Mus musculus ENSMUSG00000003808   phenylalanyl-tRNA synthetase, alpha subunit
Mycobacterium tuberculosis Rv1649   Probable phenylalanyl-tRNA synthetase, alpha chain PheS
Mycobacterium ulcerans MUL_1635   phenylalanyl-tRNA synthetase subunit alpha
Neospora caninum NCLIV_042270   hypothetical protein
Oryza sativa 4348566   Os10g0400800
Plasmodium berghei PBANKA_0203800   phenylalanine--tRNA ligase alpha subunit, putative
Plasmodium falciparum PF3D7_0109800   phenylalanine--tRNA ligase alpha subunit
Plasmodium knowlesi PKNH_0204000   phenylalanine--tRNA ligase alpha subunit, putative
Plasmodium vivax PVX_081300   phenylalanine--tRNA ligase alpha subunit, putative
Plasmodium yoelii PY00417   phenylalanyl-tRNA synthetase, putative
Saccharomyces cerevisiae YFL022C   phenylalanine--tRNA ligase subunit alpha
Schistosoma japonicum Sjp_0080790   ko:K01889 phenylalanyl-tRNA synthetase alpha chain [EC6.1.1.20A], putative
Schistosoma mansoni Smp_101240   phenylalanyl-tRNA synthetase subunit alpha
Schmidtea mediterranea mk4.039025.03  
Trypanosoma brucei gambiense Tbg972.11.15780   phenylalanyl-tRNA synthetase alpha chain, putative
Trypanosoma brucei Tb927.11.14120   phenylalanyl-tRNA synthetase alpha chain, putative
Trypanosoma congolense TcIL3000.11.14420   phenylalanyl-tRNA synthetase alpha chain, putative
Trypanosoma cruzi TcCLB.511727.140   phenylalanyl-tRNA synthetase alpha chain, putative
Toxoplasma gondii TGME49_234505   phenylalanyl-tRNA synthetase alpha chain A, putative
Treponema pallidum TP0973   phenylalanyl-tRNA synthetase subunit alpha
Theileria parva TP04_0622   phenylalanyl-tRNA synthetase alpha chain, putative
Trichomonas vaginalis TVAG_136640   phenylalanyl-tRNA synthetase alpha chain, putative
Wolbachia endosymbiont of Brugia malayi Wbm0087   phenylalanyl-tRNA synthetase subunit alpha

Essentiality

LinJ.32.0920 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1678 Mycobacterium tuberculosis essential nmpdr
Tb11.01.5710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.5710 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.5710 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1714 Escherichia coli essential goodall
CELE_T08B2.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_T08B2.9 Caenorhabditis elegans larval arrest wormbase
CELE_T08B2.9 Caenorhabditis elegans slow growth wormbase
CELE_T08B2.9 Caenorhabditis elegans sterile wormbase
CELE_T08B2.9 Caenorhabditis elegans terminal arrest variable wormbase
YFL022C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0203800 Plasmodium berghei Essential plasmo
TGME49_234505 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Type-2 restriction enzyme HindIII Compounds References
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) Phenylalanyl-tRNA synthetase alpha chain Compounds References
Streptococcus pyogenes serotype M18 (strain MGAS8232) Phenylalanyl-tRNA synthetase alpha chain Compounds References
Staphylococcus aureus Phenylalanyl-tRNA synthetase alpha chain Compounds References
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Phenylalanyl-tRNA synthetase alpha chain Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LinJ.32.0920 (Leishmania infantum), phenylalanyl-tRNA synthetase alpha chain, putative
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