Detailed view for TcCLB.506211.220

Basic information

TDR Targets ID: 44049
Trypanosoma cruzi, Associated kinase of Tb14-3-3, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.3451 | Length (AA): 720 | MW (Da): 80469 | Paralog Number: 2

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF01302   CAP-Gly domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
72 494 2acx (A) 129 532 18.00 0 1 0.55 0.19
179 424 2f2c (B) 60 301 32.00 0 1 0.52 -0.57
595 719 2cp5 (A) 1 133 30.00 0.0000026 1 0.36 0.58
660 718 1lpl (A) 162 217 41.00 0.000000024 0.99 0.61 -1.21
56 455 4ual (A) 25 371 26.00 0 0.99 0.607556 0.87
99 536 1koa (A) 5925 6309 26.00 0 1 0.730333 0.58
113 441 3c0g (B) 9 294 34.00 0 1 0.646644 0.08
123 281 4bgq (A) 18 158 43.00 0 1 0.518533 0.3
516 637 2mqa (A) 28 139 12.00 0 0.01 0.228144 -0.91
585 719 2cp5 (A) 8 133 29.00 0 1 0.3992 0.26
636 718 1lpl (A) 135 217 30.00 0.00000000071 0.9 0.505278 -0.9
638 717 1whk (A) 2 80 42.00 0.000000000089 1 0.556111 -0.05
648 718 1whl (A) 17 86 31.00 0.13 0.73 0.348311 0.11
649 720 2z0w (A) 11 80 41.00 0.0000077 1 0.4957 0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_151372)

Species Accession Gene Product
Leishmania braziliensis LbrM.19.0450   protein kinase, putative
Leishmania donovani LdBPK_190130.1   Associated kinase of Tb14-3-3, putative
Leishmania infantum LinJ.19.0130   protein kinase, putative
Leishmania major LmjF.19.0140   protein kinase, putative
Leishmania mexicana LmxM.19.0140   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.10.17930   protein kinase, putative
Trypanosoma brucei Tb927.10.14770   Associated kinase of Tb14-3-3
Trypanosoma congolense TcIL3000_10_12640   Associated kinase of Tb14-3-3, putative
Trypanosoma cruzi TcCLB.506211.220   Associated kinase of Tb14-3-3, putative
Trypanosoma cruzi TcCLB.420719.9   Associated kinase of Tb14-3-3, putative
Trypanosoma cruzi TcCLB.511287.180   Associated kinase of Tb14-3-3, putative

Essentiality

TcCLB.506211.220 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.14770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14770 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.14770 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 26.4% 276 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 27.4% 274 aa Compounds References
Patiria pectinifera Cdc2 300 aa 26.6% 271 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 29.4% 309 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 22.4% 281 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 26.6% 282 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 24.5% 319 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0091 1 0.5
0.0026 0.5 0.5
0.0032 0.5 0.5
0.0069 0.3067 1
0.0027 1 0.5
0.0012 0.5 0.5
0.0039 0.5 0.5
0.0063 0.7244 0.2543
0.0061 0.6883 0.5304
0.0003 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0039 0.9485 0.5
0.0033 1 0.5
0.0039 0.5 0.5
0.0042 0.5 0.5
0.0067 0.5 0.5
0.0004 0.5 0.5
0.0059 1 1
0.0081 0.5 0.5
0.0066 0.3101 0
0.0081 1 0.5
0.0008 0.5 0.5
0.0036 0.5 0.5
0.0022 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.3649
0.0088 0.4477 0.5
0.0056 1 0.5
0.0063 1 0.5
0.0016 0.5 0.5
0.0011 1 0.5
0.0023 0.5 0.5
0.0037 1 0.5
0.0018 0.5 0.5
0.0093 0.8828 0
0.0029 0.5 0.5
0.0062 0.6935 0
0.0016 0.5 0.5
0.0059 1 1
0.0032 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0064 0.3377 0
0.0059 1 1
0.0012 0.5 0.5
0.0007 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.506211.220 (Trypanosoma cruzi), Associated kinase of Tb14-3-3, putative
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