Detailed view for PF3D7_0605300

Basic information

TDR Targets ID: 4407
Plasmodium falciparum, serine/threonine protein kinase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.7767 | Length (AA): 353 | MW (Da): 40908 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 353 1kob (A) 23 359 23.00 0 1 1.15 -0.23
26 311 1mq4 (A) 127 387 34.00 0 1 1.15 -0.89
19 351 4lgd (D) 13 461 29.00 0 1 1.24174 -0.2
38 252 2buj (A) 36 247 25.00 0.00000005 0.98 0.714365 0.53
150 312 3vut (B) 224 372 37.00 0.000000001 0.47 0.234056 1.65
240 308 2hw6 (A) 237 334 17.00 0.97 0.25 0.395867 -0.54

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Oocyst, Female Gametocyte, Male Gametocyte. Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, sporozoite, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 40 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs. Otto TD
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_142552)

Species Accession Gene Product
Babesia bovis BBOV_II007390   Ser/Thr protein kinase, putative
Neospora caninum NCLIV_043570   PEK kinase, putative
Plasmodium berghei PBANKA_0104100   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_0605300   serine/threonine protein kinase
Plasmodium knowlesi PKNH_1145200   serine/threonine protein kinase, putative
Plasmodium vivax PVX_113435   serine/threonine protein kinase, putative
Plasmodium yoelii PY05614   serine/threonine-protein kinase ipl1
Toxoplasma gondii TGME49_210280   PEK kinase
Theileria parva TP02_0191   protein kinase, putative

Essentiality

PF3D7_0605300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_210280 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 25.7% 300 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 26.8% 183 aa Compounds References
Sus scrofa Casein kinase I isoform alpha 125 aa 31.7% 101 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 23.1% 290 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 29.0% 290 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 26.5% 287 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0064 0.3377 0
0.0063 0.7244 0.7244
0.0059 1 1
0.0022 0.5 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0056 1 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0033 1 1
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0004 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0069 0.3067 1
0.0059 1 1
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0026 0.5 0.5
0.0061 0.6883 1
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0027 1 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0066 0.3101 0
0.0062 0.6935 0
0.0059 1 1
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0008 0.5 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0011 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0605300 (Plasmodium falciparum), serine/threonine protein kinase
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