Detailed view for LmxM.18.1210

Basic information

TDR Targets ID: 447001
Leishmania mexicana, prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Source Database / ID: 

pI: 6.451 | Length (AA): 727 | MW (Da): 81232 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00587   tRNA synthetase class II core domain (G, H, P, S and T)
PF03129   Anticodon binding domain
PF04073   Aminoacyl-tRNA editing domain
PF09180   Prolyl-tRNA synthetase, C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0002161   GO:aminoacyl-tRNA editing activity  

GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004827   proline-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0006433   prolyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 176 1vjf (A) 5 167 33.00 0 1 0.725011 -1.15
172 212 4x8w (H) 1 209 56.00 0.026 0.07 0.321696 2.95
211 727 5f9z (A) 185 688 50.00 0 1 1.29544 -0.71
221 727 4hvc (A) 1016 1512 58.00 0 1 1.40069 -0.8
224 639 3ial (A) 43 470 32.00 0 1 1.01651 -0.51
228 672 1nj8 (A) 2 442 27.00 0 1 0.920105 0.05
229 669 1nj8 (A) 3 436 32.00 0 1 0.987902 -0.26
229 666 1nj1 (A) 20 452 37.00 0 1 1.05778 -0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127154)

Species Accession Gene Product
Arabidopsis thaliana AT5G52520   prolyl-tRNA synthetase
Arabidopsis thaliana AT3G62120   class II aaRS and biotin synthetases superfamily protein
Babesia bovis BBOV_IV001390   prolyl-tRNA synthetase, putative
Brugia malayi Bm1_28580   bifunctional aminoacyl-tRNA synthetase
Brugia malayi Bm1_15535   Bifunctional aminoacyl-tRNA synthetase
Candida albicans CaO19_6701   hypothetical protein
Candida albicans CaO19.13993   similar to S. cerevisiae YHR020W
Candida albicans CaO19.6701   similar to S. cerevisiae YHR020W
Caenorhabditis elegans CELE_T20H4.3   Protein PARS-1, isoform B
Cryptosporidium hominis Chro.60505   aminoacyl-tRNA synthetase
Cryptosporidium parvum cgd6_4400   proline-tRNA synthetase; class II aaRS (ybak RNA binding domain plus tRNA synthetase)
Dictyostelium discoideum DDB_G0284197   proline-tRNA ligase
Drosophila melanogaster Dmel_CG5394   Glutamyl-prolyl-tRNA synthetase
Echinococcus granulosus EgrG_000520000   glutamyl tRNA synthetase cytoplasmic
Entamoeba histolytica EHI_040400   prolyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000520000   glutamyl tRNA synthetase cytoplasmic
Giardia lamblia GL50803_15983   Prolyl-tRNA synthetase
Homo sapiens ENSG00000136628   glutamyl-prolyl-tRNA synthetase
Leishmania braziliensis LbrM.18.1310   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Leishmania infantum LinJ.18.1220   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Leishmania infantum LinJ.18.1230   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Leishmania major LmjF.18.1220   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Leishmania major LmjF.18.1210   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Leishmania mexicana LmxM.18.1210   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Leishmania mexicana LmxM.18.1220   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_11329   hypothetical protein
Loa Loa (eye worm) LOAG_05997   hypothetical protein
Loa Loa (eye worm) LOAG_09532   bifunctional aminoacyl-tRNA synthetase
Loa Loa (eye worm) LOAG_09856   hypothetical protein
Mycobacterium leprae ML1553   Probable prolyl tRNA synthetase ProS
Mus musculus ENSMUSG00000026615   glutamyl-prolyl-tRNA synthetase
Neospora caninum NCLIV_060760   prolyl-tRNA synthetase, putative
Oryza sativa 4342480   Os07g0165000
Oryza sativa 4352148   Os12g0443700
Plasmodium berghei PBANKA_1429600   proline--tRNA ligase
Plasmodium falciparum PF3D7_1213800   proline--tRNA ligase
Plasmodium knowlesi PKNH_1433300   proline--tRNA ligase, putative
Plasmodium vivax PVX_123380   proline--tRNA ligase, putative
Plasmodium yoelii PY02018   prolyl-tRNA synthetase, putative
Saccharomyces cerevisiae YHR020W   proline--tRNA ligase
Schistosoma japonicum Sjp_0054670   IPR000924,Glutamyl-tRNA synthetase, class Ic;IPR001412,Aminoacyl-tRNA synthetase, class I,domain-containing
Schistosoma japonicum Sjp_0037180   hypothetical protein
Schistosoma japonicum Sjp_0307990   expressed protein
Schistosoma japonicum Sjp_0054640   ko:K01881 prolyl-tRNA synthetase [EC6.1.1.15], putative
Schistosoma mansoni Smp_138930.2   glutamyl-tRNA synthetase cytoplasmic (glutamate--tRNA ligase) (glurs)
Schistosoma mansoni Smp_138930.1   glutamyl-tRNA synthetase cytoplasmic (glutamate--tRNA ligase) (glurs)
Trypanosoma brucei gambiense Tbg972.10.15550   prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.10.12890   bifunctional aminoacyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000_10_10990   prolyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.509805.130   prolyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.503939.80   prolyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_219850   prolyl-tRNA synthetase (ProRS)
Theileria parva TP01_0166   prolyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_053680   prolyl-tRNA synthetase, putative

Essentiality

LmxM.18.1210 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.12890 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.12890 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.12890 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.12890 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T20H4.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20H4.3 Caenorhabditis elegans larval arrest wormbase
CELE_T20H4.3 Caenorhabditis elegans larval lethal wormbase
CELE_T20H4.3 Caenorhabditis elegans slow growth wormbase
CELE_T20H4.3 Caenorhabditis elegans sterile wormbase
YHR020W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1429600 Plasmodium berghei Essential plasmo
TGME49_219850 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum (isolate 3D7) Proline--tRNA ligase Compounds References
Homo sapiens glutamyl-prolyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmxM.18.1210 (Leishmania mexicana), prolyl-tRNA synthetase, putative,bifunctional aminoacyl-tRNA synthetase, putative
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