Detailed view for PF3D7_0618300

Basic information

TDR Targets ID: 4534
Plasmodium falciparum, 60S ribosomal protein L27a, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 11.2314 | Length (AA): 148 | MW (Da): 16723 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00828   Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

Gene Ontology

Mouse over links to read term descriptions.
GO:0015934   large ribosomal subunit  
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 147 1vq8 (L) 2 143 29.00 0 0.92 1.06 1.51
35 146 1vq8 (O) 19 98 25.00 0 0.97 0.6 0.47
5 147 1vq8 (L) 1 143 21.00 0.000000000028 0.37 1.04562 0.98
8 142 1vq8 (L) 2 138 32.00 0.0000047 0.78 1.04046 1.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst, Ring, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_127016)

Species Accession Gene Product
Arabidopsis thaliana AT1G70600   60S ribosomal protein L27a-3
Arabidopsis thaliana AT1G23290   60S ribosomal protein L27a-2
Babesia bovis BBOV_III006560   ribosomal protein L15, putative
Brugia malayi Bm1_04910   60S ribosomal protein L27a
Caenorhabditis elegans CELE_Y37E3.8   Protein Y37E3.8, isoform A
Cryptosporidium hominis Chro.30443   ribosomal protein L22
Cryptosporidium parvum cgd3_3930   60S ribosomal protein L27A or L27a
Dictyostelium discoideum DDB_G0292388   S60 ribosomal protein L27a
Drosophila melanogaster Dmel_CG15442   Ribosomal protein L27A
Entamoeba histolytica EHI_169100   ribosomal protein L27a, putative
Entamoeba histolytica EHI_069490   ribosomal protein L27a, putative
Entamoeba histolytica EHI_192090   ribosomal protein L27a, putative
Giardia lamblia GL50803_16310   Ribosomal protein L27a
Homo sapiens ENSG00000166441   ribosomal protein L27a
Leishmania braziliensis LbrM.34.3740   60S ribosomal protein L27A/L29, putative
Leishmania braziliensis LbrM.34.3760   60S ribosomal protein L27A/L29, putative
Leishmania donovani LdBPK_353810.1   60S ribosomal protein L27A/L29, putative
Leishmania infantum LinJ.35.3830   60S ribosomal protein L27A/L29, putative
Leishmania infantum LinJ.35.3810   60S ribosomal protein L27A/L29, putative
Leishmania major LmjF.35.3760   60S ribosomal protein L27A/L29, putative
Leishmania major LmjF.35.3780   60S ribosomal protein L27A/L29, putative
Leishmania mexicana LmxM.34.3760   60S ribosomal protein L27A/L29, putative
Leishmania mexicana LmxM.34.3780   60S ribosomal protein L27A/L29, putative
Loa Loa (eye worm) LOAG_09561   60S ribosomal protein L27a
Mus musculus 546052   predicted gene 5908
Mus musculus ENSMUSG00000046364   ribosomal protein L27A
Neospora caninum NCLIV_054570   60S ribosomal protein Ll28B 27a, related
Oryza sativa 4328472   Os02g0175600
Oryza sativa 4333083   Os03g0408300
Oryza sativa 4343884   Os07g0613200
Onchocerca volvulus OVOC1439   60S ribosomal protein L27a homolog
Plasmodium berghei PBANKA_1117500   ribosomal protein L27a, putative
Plasmodium falciparum PF3D7_0618300   60S ribosomal protein L27a, putative
Plasmodium knowlesi PKNH_1132100   60S ribosomal protein L27a, putative
Plasmodium vivax PVX_114040   60S ribosomal protein L27a, putative
Plasmodium yoelii PY03375   ribosomal protein L27a
Saccharomyces cerevisiae YGL103W   ribosomal 60S subunit protein L28
Schmidtea mediterranea mk4.037030.00   60S ribosomal protein L27a
Trypanosoma brucei gambiense Tbg972.9.6830   60S ribosomal protein L27a,60S ribosomal protein L28,60S ribosomal protein L29, putative
Trypanosoma brucei gambiense Tbg972.9.6810   60S ribosomal protein L27a,60S ribosomal protein L28,60S ribosomal protein L29
Trypanosoma brucei Tb927.9.11490   60S ribosomal protein L29
Trypanosoma brucei Tb427tmp.211.2650   60S ribosomal protein L27a
Trypanosoma congolense TcIL3000_0_56640   60S ribosomal protein L27a
Trypanosoma cruzi TcCLB.508461.510   60S ribosomal protein L27A/L29, putative
Trypanosoma cruzi TcCLB.508461.500   60S ribosomal protein L27A/L29, putative
Toxoplasma gondii TGME49_310490   ribosomal protein RPL27A
Theileria parva TP02_0317   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_143790   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_121550   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_401890   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_346410   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_199230   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_300490   60S ribosomal protein L27a, putative

Essentiality

PF3D7_0618300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2650 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.2650 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2650 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.2650 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y37E3.8 Caenorhabditis elegans embryonic lethal wormbase
YGL103W Saccharomyces cerevisiae inviable yeastgenome
TGME49_310490 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0618300 (Plasmodium falciparum), 60S ribosomal protein L27a, putative
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