Detailed view for TcCLB.511517.60

Basic information

TDR Targets ID: 46107
Trypanosoma cruzi, C-1-tetrahydrofolate synthase, cytoplasmic, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1691 | Length (AA): 649 | MW (Da): 70406 | Paralog Number: 3

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01268   Formate--tetrahydrofolate ligase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004329   formate-tetrahydrofolate ligase activity  
GO:0009396   folic acid and derivative biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
39 648 4jim (A) 4 558 54.00 0 1 1.51601 -0.31
189 251 2eo2 (A) 6 71 51.00 0 1 0.838372 -1.76

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126671)

Species Accession Gene Product
Arabidopsis thaliana AT1G50480   Formate--tetrahydrofolate ligase
Arabidopsis thaliana AT3G12290   amino acid dehydrogenase family protein
Arabidopsis thaliana AT4G00620   Amino acid dehydrogenase family protein
Candida albicans CaO19.2364   likely C1-tetrahydrofolate synthase similar to S. cerevisiae ADE3 (YGR204W) involved in purine base and histidine biosynthesis
Candida albicans CaO19.7534   Mitochondrial C1-tetrahydroflate synthase
Candida albicans CaO19.9900   likely C1-tetrahydrofolate synthase similar to S. cerevisiae ADE3 (YGR204W) involved in purine base and histidine biosynthesis
Caenorhabditis elegans CELE_K07E3.3   Protein DAO-3
Caenorhabditis elegans CELE_K07E3.4   Protein K07E3.4, isoform A
Chlamydia trachomatis CT_078   bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Dictyostelium discoideum DDB_G0290397   formate-dihydrofolate ligase
Dictyostelium discoideum DDB_G0283121   methenyl tetrahydrofolate cyclohydrolase / NADP-dependent methylene H4F dehydrogenase
Drosophila melanogaster Dmel_CG4067   pugilist
Drosophila melanogaster Dmel_CG18466   NAD-dependent methylenetetrahydrofolate dehydrogenase
Escherichia coli b0529   bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase
Echinococcus granulosus EgrG_001195300   methylenetetrahydrofolate dehydrogenase
Echinococcus multilocularis EmuJ_001195300   methylenetetrahydrofolate dehydrogenase
Homo sapiens ENSG00000100714   methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate syn
Homo sapiens ENSG00000120254   methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Homo sapiens 10797   methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Homo sapiens ENSG00000163738   methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like
Leishmania braziliensis LbrM.26.0350   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Leishmania braziliensis LbrM.30.2550   formate--tetrahydrofolate ligase
Leishmania donovani LdBPK_260310.1   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Leishmania donovani LdBPK_302590.1   formate--tetrahydrofolate ligase
Leishmania infantum LinJ.30.2590   formate--tetrahydrofolate ligase
Leishmania infantum LinJ.26.0310   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Leishmania major LmjF.30.2600   formate--tetrahydrofolate ligase
Leishmania major LmjF.26.0320   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Leishmania mexicana LmxM.26.0320   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Leishmania mexicana LmxM.29.2600   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Mycobacterium leprae ML0674   Probable bifunctional protein fold: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase FolD
Mus musculus ENSMUSG00000005667   methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase
Mus musculus ENSMUSG00000040675   methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Mus musculus ENSMUSG00000021048   methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synth
Mus musculus ENSMUSG00000029376   methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like
Mycobacterium tuberculosis Rv3356c   Probable bifunctional protein FolD: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase
Mycobacterium ulcerans MUL_1428   bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase
Oryza sativa 4347204   Os09g0446800
Oryza sativa 4328117   Os02g0120900
Oryza sativa 4337829   Os05g0150800
Saccharomyces cerevisiae YGR204W   trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase AD
Saccharomyces cerevisiae YBR084W   trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MI
Schistosoma japonicum Sjp_0024520   ko:K00288 methylenetetrahydrofolate dehydrogenase (NADP+) [EC1.5.1.5], putative
Schistosoma mansoni Smp_147650   formate-tetrahydrofolate ligase
Schmidtea mediterranea mk4.001298.09   C-1-tetrahydrofolate synthase, cytoplasmic
Schmidtea mediterranea mk4.001298.06  
Schmidtea mediterranea mk4.001298.08  
Schmidtea mediterranea mk4.038149.03   Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Schmidtea mediterranea mk4.007154.02   C-1-tetrahydrofolate synthase, cytoplasmic
Trypanosoma brucei gambiense Tbg972.7.1600   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Trypanosoma brucei Tb927.7.1600   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Trypanosoma congolense TcIL3000_7_1130   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Trypanosoma cruzi TcCLB.511517.60   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Trypanosoma cruzi TcCLB.511741.50   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Trypanosoma cruzi TcCLB.509509.20   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Trypanosoma cruzi TcCLB.508207.240   C-1-tetrahydrofolate synthase, cytoplasmic, putative
Treponema pallidum TP0732   methylenetetrahydrofolate dehydrogenase (folD)
Wolbachia endosymbiont of Brugia malayi Wbm0073   bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase

Essentiality

TcCLB.511517.60 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.1600 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1600 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1600 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.1600 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0529 Escherichia coli essential goodall
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for 5,10-Methylenetetrahydrofolate Dehydrogenase (1.5.1.5 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.511517.60 (Trypanosoma cruzi), C-1-tetrahydrofolate synthase, cytoplasmic, putative
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