Detailed view for PF3D7_0629300

Basic information

TDR Targets ID: 4642
Plasmodium falciparum, phospholipase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.6371 | Length (AA): 863 | MW (Da): 99231 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02450   Lecithin:cholesterol acyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0020011   apicoplast  
GO:0008374   O-acyltransferase activity  
GO:0004607   phosphatidylcholine-sterol O-acyltransferase activity  
GO:0006629   lipid metabolic process  

Metabolic Pathways

Glycerophospholipid metabolism (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
536 645 1isp (A) 4 110 12.00 0.00000023 0.77 0.33 -1.06
37 173 2i1k (A) 298 449 6.00 0.9 0 0.282949 -0.88
104 211 3ult (A) 6 116 30.00 0.39 0.91 0.604145 -1.35
444 862 4x90 (A) 5 379 31.00 0 1 0.690516 0.08
2 73 3ult (A) 41 112 8.00 0.041 0.01 -0.0178816 0.91
440 858 4x90 (A) 5 379 31.00 0 1 0.691576 0.08
522 607 1a8q (A) 12 97 13.00 0.74 0.04 0.152416 -0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 32 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_128356)

Species Accession Gene Product
Arabidopsis thaliana AT1G27480   Lecithin-cholesterol acyltransferase-like 1
Brugia malayi Bm1_48320   Lecithin:cholesterol acyltransferase family protein
Caenorhabditis elegans CELE_M05B5.4   Protein M05B5.4
Drosophila melanogaster Dmel_CG31683   CG31683 gene product from transcript CG31683-RA
Drosophila melanogaster Dmel_CG18858   CG18858 gene product from transcript CG18858-RB
Echinococcus granulosus EgrG_000592400   phospholipase A
Entamoeba histolytica EHI_021300   lecithin:cholesterol acyltransferase domain-containing protein
Entamoeba histolytica EHI_020250   lecithin:cholesterol acyltransferase domain-containing protein
Entamoeba histolytica EHI_084690   lecithin:cholesterol acyltransferase domain-containing protein
Entamoeba histolytica EHI_035750   lecithin:cholesterol acyltransferase domain-containing protein
Echinococcus multilocularis EmuJ_000592400   phospholipase A
Homo sapiens ENSG00000103066   phospholipase A2, group XV
Loa Loa (eye worm) LOAG_14369   Lecithin:cholesterol acyltransferase
Loa Loa (eye worm) LOAG_12112   hypothetical protein
Mus musculus ENSMUSG00000031903   phospholipase A2, group XV
Neospora caninum NCLIV_034880   1-O-acylceramide synthase, putative
Oryza sativa 4333995   Os03g0730000
Onchocerca volvulus OVOC1154  
Plasmodium berghei PBANKA_1128100   phospholipase
Plasmodium falciparum PF3D7_0629300   phospholipase, putative
Plasmodium knowlesi PKNH_1120200   phospholipase, putative
Plasmodium vivax PVX_114565   phospholipase, putative
Plasmodium yoelii PY04170   Lecithin:cholesterol acyltransferase, putative
Schistosoma japonicum Sjp_0214980   ko:K06129 lysophospholipase III, putative
Schistosoma japonicum Sjp_0029100   1-O-acylceramide synthase precursor, putative
Schistosoma japonicum Sjp_0128450   IPR003386,Lecithin:cholesterol acyltransferase,domain-containing
Schistosoma japonicum Sjp_0079120   ko:K00650 lecithin-cholesterol acyltransferase [EC2.3.1.43], putative
Schistosoma japonicum Sjp_0079060   ko:K06129 lysophospholipase III, putative
Schistosoma japonicum Sjp_0029090   1-O-acylceramide synthase precursor, putative
Schistosoma mansoni Smp_166530.1   phospholipase A
Schistosoma mansoni Smp_166500.2   phospholipase A
Schistosoma mansoni Smp_031190   phospholipase A
Schistosoma mansoni Smp_166540   kinase
Schistosoma mansoni Smp_166500.3   phospholipase A
Schistosoma mansoni Smp_031180   phospholipase A
Schistosoma mansoni Smp_160880   phospholipase A
Schistosoma mansoni Smp_166530.2   phospholipase A
Schmidtea mediterranea mk4.027759.00  
Schmidtea mediterranea mk4.002786.05  
Schmidtea mediterranea mk4.004417.00  
Schmidtea mediterranea mk4.008479.00  
Schmidtea mediterranea mk4.014957.00  
Schmidtea mediterranea mk4.001489.05  
Schmidtea mediterranea mk4.026737.00  
Schmidtea mediterranea mk4.001489.04  
Schmidtea mediterranea mk4.045654.00  
Schmidtea mediterranea mk4.030313.00  
Schmidtea mediterranea mk4.013269.00  
Schmidtea mediterranea mk4.020442.00  
Schmidtea mediterranea mk4.020164.00  
Schmidtea mediterranea mk4.022053.00  
Toxoplasma gondii TGME49_272420   phosphatidylcholine-sterol O-acyltransferase, putative
Trichomonas vaginalis TVAG_306360   phosphatidylcholine-sterol acyltransferase, putative
Trichomonas vaginalis TVAG_148680   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_407760   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_195450   1-O-acylceramide synthase precursor, putative
Trichomonas vaginalis TVAG_390810   phosphatidylcholine-sterol acyltransferase, putative
Trichomonas vaginalis TVAG_395490   phospholipid:diacylglycerol acyltransferase, putative
Trichomonas vaginalis TVAG_360450   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_167100   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_039060   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_452180   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_052070   Phosphatidylcholine-sterol acyltransferase precursor, putative
Trichomonas vaginalis TVAG_390800   conserved hypothetical protein
Trichomonas vaginalis TVAG_339350   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_140960   phosphatidylcholine-sterol acyltransferase, putative
Trichomonas vaginalis TVAG_167090   1-O-acylceramide synthase, putative
Trichomonas vaginalis TVAG_240800   phosphatidylcholine-sterol acyltransferase, putative
Trichomonas vaginalis TVAG_340750   phosphatidylcholine-sterol acyltransferase, putative

Essentiality

PF3D7_0629300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1128100 Plasmodium berghei Dispensable plasmo
TGME49_272420 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) multi-cellular organism (CARO:0000012) sporozoite (PLO:0018) inferred from loss-of-function mutant phenotype (ECO:0000016) Plasmodium berghei No drug identifiers listed for this gene.
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 15590623
growth (GO:0040007) decreased time (PATO:0000716) multi-cellular organism (CARO:0000012) sporozoite (PLO:0018) inferred from loss-of-function mutant phenotype (ECO:0000016) Plasmodium falciparum No drug identifiers listed for this gene.
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 15590623

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

3 literature references were collected for this gene.

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Gene identifier PF3D7_0629300 (Plasmodium falciparum), phospholipase, putative
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