Detailed view for PF3D7_0905700

Basic information

TDR Targets ID: 4734
Plasmodium falciparum, autophagy-related protein 3, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.3154 | Length (AA): 313 | MW (Da): 36793 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03986   Autophagocytosis associated protein (Atg3), N-terminal domain
PF03987   Autophagocytosis associated protein, active-site domain
PF10381   Autophagocytosis associated protein C-terminal

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Regulation of autophagy (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
30 307 3vx8 (B) 26 304 49.00 0 1 0.737173 0.95
30 301 3vx8 (B) 26 304 49.00 0 1 0.78181 0.82
31 100 3vx8 (B) 27 199 50.00 0 0.42 1.09631 0.09
78 119 4iiw (A) 46 87 31.00 0.63 0.18 0.745663 -0.82

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, gametocyte, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Oocyst, Ring, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs. Otto TD
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_128240)

Species Accession Gene Product
Arabidopsis thaliana AT5G61500   autophagy-related protein 3
Brugia malayi Bm1_46895   Autophagocytosis associated protein, C-terminal domain containing protein
Candida albicans CaO19.6020   similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation re
Candida albicans CaO19.13441   similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation re
Caenorhabditis elegans CELE_Y55F3AM.4   Protein ATG-3
Cryptosporidium hominis Chro.80308   autophagocytosis protein
Cryptosporidium parvum cgd8_2630   APG10/ Aut1p like like autophagocytosis protein involved in vacuolar transport
Dictyostelium discoideum DDB_G0277319   autophagy protein 3
Drosophila melanogaster Dmel_CG6877   Autophagy-specific gene 3
Echinococcus granulosus EgrG_000488800   autophagy protein 3
Entamoeba histolytica EHI_194330   autophagocytosis protein, putative
Echinococcus multilocularis EmuJ_000488800   autophagy protein 3
Homo sapiens ENSG00000144848   autophagy related 3
Leishmania braziliensis LbrM.33.0300   autophagocytosis protein, putative
Leishmania donovani LdBPK_330320.1   autophagocytosis protein, putative
Leishmania infantum LinJ.33.0320   autophagocytosis protein, putative
Leishmania major LmjF.33.0295   autophagocytosis protein, putative
Loa Loa (eye worm) LOAG_01587   hypothetical protein
Mus musculus ENSMUSG00000022663   autophagy related 3
Neospora caninum NCLIV_050410   autophagocytosis associated protein, putative
Oryza sativa 9270245   Os10g0560450
Oryza sativa 4327346   Os01g0200000
Plasmodium berghei PBANKA_0415700   autophagy-related protein 3, putative
Plasmodium falciparum PF3D7_0905700   autophagy-related protein 3, putative
Plasmodium knowlesi PKNH_0703400   autophagy-related protein 3, putative
Plasmodium yoelii PY04567   unnamed protein product-related
Saccharomyces cerevisiae YNR007C   Atg3p
Schistosoma japonicum Sjp_0029700   ko:K08343 autophagy-related protein 3, putative
Schistosoma mansoni Smp_077400   autophagocytosis protein aut1-related
Schmidtea mediterranea mk4.000398.03   Ubiquitin-like-conjugating enzyme ATG3
Trypanosoma brucei gambiense Tbg.972.2.410   autophagocytosis associated protein, putative
Trypanosoma brucei Tb927.2.1890   E2-like ubiquitin-conjugation enzyme
Trypanosoma congolense TcIL3000_0_09690   E2-like ubiquitin-conjugation enzyme
Trypanosoma congolense TcIL3000_0_03190   E2-like ubiquitin-conjugation enzyme
Trypanosoma cruzi TcCLB.510257.90   autophagocytosis associated protein, putative
Trypanosoma cruzi TcCLB.509213.130   autophagocytosis associated protein, putative
Toxoplasma gondii TGME49_236110   autophagy-related protein 3 atg3, putative
Theileria parva TP03_0261   hypothetical protein
Trichomonas vaginalis TVAG_447140   conserved hypothetical protein

Essentiality

PF3D7_0905700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.1890 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.2.1890 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.1890 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.1890 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0415700 Plasmodium berghei Essential plasmo
TGME49_236110 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_0905700 (Plasmodium falciparum), autophagy-related protein 3, putative
Title for this comment
Comment