Detailed view for TcCLB.506247.410

Basic information

TDR Targets ID: 47391
Trypanosoma cruzi, protein-l-isoaspartate o-methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.5925 | Length (AA): 239 | MW (Da): 25804 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01135   Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

Gene Ontology

Mouse over links to read term descriptions.
GO:0004719   protein-L-isoaspartate (D-aspartate) O-methyltransferase activity  
GO:0006464   protein modification process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 238 1i1n (A) 2 224 39.00 0 1 1.47 -1.3
3 238 1i1n (A) 2 224 39.00 0 1 1.52895 -1.1
7 229 4o29 (A) 0 204 40.00 0 1 1.44455 -1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127195)

Species Accession Gene Product
Arabidopsis thaliana AT5G50240   protein-l-isoaspartate methyltransferase 2
Arabidopsis thaliana AT3G48330   protein-L-isoaspartate O-methyltransferase
Brugia malayi Bm1_50950   L-isoaspartate
Brugia malayi Bm1_52680   L-isoaspartate
Brugia malayi Bm1_50945   protein-L-isoaspartate
Caenorhabditis elegans CELE_C10F3.5   Protein PCM-1, isoform B
Dictyostelium discoideum DDB_G0280979   hypothetical protein
Drosophila melanogaster Dmel_CG2152   Protein-L-isoaspartate (D-aspartate) O-methyltransferase
Escherichia coli b2743   L-isoaspartate protein carboxylmethyltransferase type II
Echinococcus granulosus EgrG_001133400   protein l isoaspartate o methyltransferase
Echinococcus multilocularis EmuJ_001133400   protein l isoaspartate o methyltransferase
Homo sapiens ENSG00000120265   protein-L-isoaspartate (D-aspartate) O-methyltransferase
Leishmania braziliensis LbrM.35.6910   protein-l-isoaspartate o-methyltransferase, putative
Leishmania donovani LdBPK_366860.1   protein-l-isoaspartate o-methyltransferase, putative
Leishmania infantum LinJ.36.6860   protein-l-isoaspartate o-methyltransferase, putative
Leishmania major LmjF.36.6550   protein-l-isoaspartate o-methyltransferase, putative
Leishmania mexicana LmxM.36.6550   protein-l-isoaspartate o-methyltransferase, putative
Loa Loa (eye worm) LOAG_05196   L-isoaspartate
Loa Loa (eye worm) LOAG_00050   L-isoaspartate
Loa Loa (eye worm) LOAG_05197   protein-L-isoaspartate
Mus musculus 18537   protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
Neospora caninum NCLIV_057890   Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), related
Oryza sativa 4346293   Os08g0557000
Plasmodium berghei PBANKA_1012200   protein-L-isoaspartate(D-aspartate) O-methyltransferase, putative
Plasmodium falciparum PF3D7_1432700   protein-L-isoaspartate(D-aspartate) O-methyltransferase, putative
Plasmodium knowlesi PKNH_0419300   protein-L-isoaspartate(D-aspartate) O-methyltransferase, putative
Plasmodium vivax PVX_084910   protein-L-isoaspartate O-methyltransferase beta-aspartate methyltransferase, putative
Plasmodium yoelii PY05016   protein-l-isoaspartate o-methyltransferase-related
Schistosoma japonicum Sjp_0209810   ko:K00573 protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC2.1.1.77], putative
Schistosoma mansoni Smp_058140.3   protein-l-isoaspartate o-methyltransferase
Schistosoma mansoni Smp_058140.1   protein-l-isoaspartate o-methyltransferase
Schistosoma mansoni Smp_058140.4   protein-l-isoaspartate o-methyltransferase
Schistosoma mansoni Smp_058140.2   protein-l-isoaspartate o-methyltransferase
Schmidtea mediterranea mk4.080464.01  
Schmidtea mediterranea mk4.001403.00  
Schmidtea mediterranea mk4.001433.01  
Schmidtea mediterranea mk4.001467.05   Protein-L-isoaspartate O-methyltransferase
Trypanosoma brucei gambiense Tbg972.10.9610   protein-L-isoaspartate, putative
Trypanosoma brucei Tb927.10.7850   protein-L-isoaspartate, putative
Trypanosoma congolense TcIL3000_10_6720   protein-L-isoaspartate, putative
Trypanosoma cruzi TcCLB.506247.410   protein-l-isoaspartate o-methyltransferase, putative
Trypanosoma cruzi TcCLB.503779.40   protein-l-isoaspartate o-methyltransferase, putative
Toxoplasma gondii TGME49_315130   L-isoaspartyl protein carboxyl methyltransferase family protein

Essentiality

TcCLB.506247.410 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7850 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.7850 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.7850 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.7850 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2743 Escherichia coli non-essential goodall
CELE_C10F3.5 Caenorhabditis elegans embryonic lethal wormbase
TGME49_315130 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tcru004507AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru004507; Recombinant protein: full-length; Source: T cruzi; protein-L-isoaspartate O-methyltransferase ;

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.506247.410 (Trypanosoma cruzi), protein-l-isoaspartate o-methyltransferase, putative
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