Detailed view for TcCLB.511881.30

Basic information

TDR Targets ID: 47485
Trypanosoma cruzi, GIY-YIG catalytic domain containing protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7244 | Length (AA): 530 | MW (Da): 59703 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01541   GIY-YIG catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0033557   Slx1-Slx4 complex  
GO:0017108   5'-flap endonuclease activity  
GO:0005622   intracellular  
GO:0004518   nuclease activity  
GO:0006281   DNA repair  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 97 1zg2 (A) 7 89 37.00 0 0.8 0.46 0.4
377 516 4uos (A) 46 184 6.00 0.58 0.01 0.424251 -1.35
454 503 5ffp (A) 82 136 38.00 0.94 0.01 0.38984 -0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128732)

Species Accession Gene Product
Arabidopsis thaliana AT2G30350   Excinuclease ABC, C subunit, N-terminal
Brugia malayi Bm1_35165   GIY-YIG catalytic domain containing protein
Candida albicans CaO19.8587   similar to S. cerevisiae SLX1 (YBR228W) subunit of DNA replication structure-specific endonuclease
Candida albicans CaO19.972   similar to S. cerevisiae SLX1 (YBR228W) subunit of DNA replication structure-specific endonuclease
Caenorhabditis elegans CELE_F56A3.2   Protein SLX-1
Cryptosporidium hominis Chro.20460   hypothetical protein
Cryptosporidium parvum cgd2_4280   holiday junction resolvase, S1x1p, URI domain nuclease
Drosophila melanogaster Dmel_CG18271   nuclease slx1
Echinococcus granulosus EgrG_001040600   structure specific endonuclease subunit
Entamoeba histolytica EHI_156250   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_001040600   structure specific endonuclease subunit
Giardia lamblia GL50803_16475   Hypothetical protein
Homo sapiens 101929864   structure-specific endonuclease subunit SLX1-like
Homo sapiens ENSG00000132207   SLX1 structure-specific endonuclease subunit homolog A (S. cerevisiae)
Homo sapiens ENSG00000181625   SLX1 structure-specific endonuclease subunit homolog B (S. cerevisiae)
Leishmania braziliensis LbrM.25.1030   hypothetical protein, conserved
Leishmania donovani LdBPK_251370.1   GIY-YIG catalytic domain containing protein, putative
Leishmania infantum LinJ.25.1370   hypothetical protein, conserved
Leishmania major LmjF.25.1330   hypothetical protein, conserved
Leishmania mexicana LmxM.25.1330   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_11802   GIY-YIG catalytic domain-containing protein
Loa Loa (eye worm) LOAG_16021   hypothetical protein
Mus musculus ENSMUSG00000059772   SLX1 structure-specific endonuclease subunit homolog B (S. cerevisiae)
Oryza sativa 4342773   Os07g0230500
Saccharomyces cerevisiae YBR228W   Slx1p
Schistosoma japonicum Sjp_0087280   GIY-YIG domain-containing protein 1, putative
Schistosoma mansoni Smp_106040   hypothetical protein
Schmidtea mediterranea mk4.000040.02   Structure-specific endonuclease subunit SLX1 homolog
Trypanosoma brucei gambiense Tbg972.3.970   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0598   GIY-YIG catalytic domain containing protein, putative
Trypanosoma congolense TcIL3000_0_20040   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_3_470   hypothetical protein
Trypanosoma cruzi TcCLB.509453.60   GIY-YIG catalytic domain containing protein, putative
Trypanosoma cruzi TcCLB.511881.30   GIY-YIG catalytic domain containing protein, putative
Trichomonas vaginalis TVAG_096720   spap27g11.15 protein, putative

Essentiality

TcCLB.511881.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1220 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.1220 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.1220 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F56A3.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_F56A3.2 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511881.30 (Trypanosoma cruzi), GIY-YIG catalytic domain containing protein, putative
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