Detailed view for NCLIV_044930

Basic information

TDR Targets ID: 475433
Neospora caninum, Sec7 domain containing protein, related
Source Database / ID: 

pI: 6.3933 | Length (AA): 3713 | MW (Da): 399931 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01369   Sec7 domain
PF09324   Domain of unknown function (DUF1981)
PF12783   Guanine nucleotide exchange factor in Golgi transport N-terminal
PF16213   Dimerisation and cyclophilin-binding domain of Mon2

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  
GO:0005086   ARF guanyl-nucleotide exchange factor activity  
GO:0032012   regulation of ARF protein signal transduction  

Metabolic Pathways

Endocytosis (KEGG)

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
120 273 5cwk (A) 22 172 14.00 0 0.04 0.302076 -1.67
288 564 5ee5 (A) 25 226 25.00 0 1 -0.234797 0.39
414 557 5has (D) 36 255 26.00 0 0.98 0.419383 -1.09
438 532 5has (C) 63 179 33.00 0.0084 0.99 0.457386 -0.95
781 1003 5has (C) 57 454 17.00 0 0.48 0.0736592 0.14
1186 1585 2r09 (A) 58 392 29.00 0 1 0.25843 0.3
1190 1418 1ku1 (A) 0 759 29.00 0 1 0.390375 -0.88
1192 1414 4oiy (A) 825 1009 46.00 0 1 0.431659 -0.62
1589 1753 5a61 (A) 173 331 30.00 0.45 0.45 0.201238 0.76
2895 3119 5cwi (A) 10 232 16.00 0 0.34 0.279198 -0.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127540)

Species Accession Gene Product
Arabidopsis thaliana AT4G38200   SEC7-like guanine nucleotide exchange family protein
Arabidopsis thaliana AT1G01960   brefeldin A-inhibited guanine nucleotide-exchange protein 3
Arabidopsis thaliana AT3G60860   SEC7-like guanine nucleotide exchange family protein
Arabidopsis thaliana AT4G35380   SEC7-like guanine nucleotide exchange family protein
Arabidopsis thaliana AT3G43300   brefeldin A-inhibited guanine nucleotide-exchange protein 5
Brugia malayi Bm1_30405   symbol
Candida albicans CaO19.5947   likely guanine nucleotide exchange protein for ARF similar to S. cerevisiae SEC7 (YDR170C) involved in ER to Golgi transport
Candida albicans CaO19.13368   likely guanine nucleotide exchange protein for ARF similar to S. cerevisiae SEC7 (YDR170C) involved in ER to Golgi transport
Caenorhabditis elegans CELE_Y6B3A.1   Protein AGEF-1, isoform B
Dictyostelium discoideum DDB_G0273831   SEC7-like domain-containing protein
Dictyostelium discoideum DDB_G0290369   Arf guanyl-nucleotide exchange factor
Dictyostelium discoideum DDB_G0273101   SEC7-like domain-containing protein
Drosophila melanogaster Dmel_CG7578   Sec71 ortholog (S. cerevisiae)
Echinococcus granulosus EgrG_000708200   brefeldin A inhibited guanine
Entamoeba histolytica EHI_048270   Sec7 domain protein
Entamoeba histolytica EHI_024590   Sec7 domain protein
Entamoeba histolytica EHI_038940   Sec7 domain protein
Entamoeba histolytica EHI_178690   brefeldin a-inhibited guanine nucleotide-exchange protein, putative
Echinococcus multilocularis EmuJ_000708200   brefeldin A inhibited guanine
Homo sapiens ENSG00000124198   ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Homo sapiens ENSG00000066777   ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Loa Loa (eye worm) LOAG_07639   hypothetical protein
Loa Loa (eye worm) LOAG_08344   hypothetical protein
Mus musculus ENSMUSG00000067851   ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Mus musculus ENSMUSG00000074582   ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Neospora caninum NCLIV_044930   Sec7 domain containing protein, related
Oryza sativa 4341555   Os06g0622800
Oryza sativa 4332241   Os03g0246800
Saccharomyces cerevisiae YDR170C   Sec7p
Schistosoma japonicum Sjp_0074620   Brefeldin A-inhibited guanine nucleotide-exchange protein 1, putative
Schistosoma japonicum Sjp_0074610   Brefeldin A-inhibited guanine nucleotide-exchange protein 1, putative
Schistosoma mansoni Smp_135280   brefeldin A-inhibited guanine nucleotide-exchange protein
Schmidtea mediterranea mk4.005819.02   Putative brefeldin A-inhibited guanine nucleotide-exchange protein
Schmidtea mediterranea mk4.002394.02   Putative brefeldin A-inhibited guanine nucleotide-exchange protein
Schmidtea mediterranea mk4.002394.01  
Schmidtea mediterranea mk4.002394.03   Putative brefeldin A-inhibited guanine nucleotide-exchange protein
Schmidtea mediterranea mk4.005819.00   Putative brefeldin A-inhibited guanine nucleotide-exchange protein
Trypanosoma cruzi TcCLB.510187.448   hypothetical protein, conserved
Toxoplasma gondii TGME49_228660   Sec7 domain-containing protein
Trichomonas vaginalis TVAG_411170   guanyl-nucleotide exchange factor, putative
Trichomonas vaginalis TVAG_333810   guanine nucleotide-exchange, putative
Trichomonas vaginalis TVAG_321160   guanine nucleotide-exchange, putative
Trichomonas vaginalis TVAG_210270   brefeldin A-inhibited guanine nucleotide-exchange protein, putative

Essentiality

NCLIV_044930 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y6B3A.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y6B3A.1 Caenorhabditis elegans larval arrest wormbase
CELE_Y6B3A.1 Caenorhabditis elegans larval lethal wormbase
CELE_Y6B3A.1 Caenorhabditis elegans slow growth wormbase
CELE_Y6B3A.1 Caenorhabditis elegans sterile wormbase
YDR170C Saccharomyces cerevisiae inviable yeastgenome
TGME49_228660 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Human immunodeficiency virus 1 GP41 124 aa 21.0% 124 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier NCLIV_044930 (Neospora caninum), Sec7 domain containing protein, related
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