Detailed view for NCLIV_040330

Basic information

TDR Targets ID: 478109
Neospora caninum, sodium-dependent transporter, putative
Source Database / ID: 

pI: 8.7656 | Length (AA): 1049 | MW (Da): 110182 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 14

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00209   Sodium:neurotransmitter symporter family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0005328   neurotransmitter:sodium symporter activity  
GO:0006836   neurotransmitter transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 135 2mqa (A) 22 139 13.00 0 0.01 0.109641 -0.2
232 725 4xp4 (A) 30 502 35.00 0 1 0.738525 0.03
232 759 5i6x (A) 82 561 32.00 0 1 0.749937 -0.06
345 815 5i6x (A) 141 617 20.00 0 0.54 0.554299 0.88
389 815 4xp4 (A) 130 599 24.00 0 0.7 0.539354 1.17
435 782 3o7q (A) 52 431 18.00 0 0.81 0.345645 0.98

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_224012)

Species Accession Gene Product
Neospora caninum NCLIV_040330   sodium-dependent transporter, putative
Toxoplasma gondii TGME49_264870   Sodium:neurotransmitter symporter family protein

Essentiality

NCLIV_040330 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_264870 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Dopamine transporter 619 aa 32.3% 498 aa Compounds References
Macaca fascicularis Dopamine transporter 620 aa 31.1% 501 aa Compounds References
Rattus norvegicus Creatine transporter 635 aa 28.3% 569 aa Compounds References
Rattus norvegicus GABA transporter 1 599 aa 28.9% 532 aa Compounds References
Rattus norvegicus Norepinephrine transporter 597 aa 30.4% 507 aa Compounds References
Macaca mulatta Serotonin transporter 630 aa 27.2% 544 aa Compounds References
Macaca mulatta Transporter 620 aa 31.1% 501 aa Compounds References
Rattus norvegicus GABA transporter 614 aa 28.9% 526 aa Compounds References
Rattus norvegicus Transporter 617 aa 30.6% 507 aa Compounds References
Rattus norvegicus GABA transporter 2 602 aa 28.8% 503 aa Compounds References
Bos taurus Sodium- and chloride-dependent glycine transporter 1 638 aa 28.9% 512 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier NCLIV_040330 (Neospora caninum), sodium-dependent transporter, putative
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