Detailed view for NCLIV_021920

Basic information

TDR Targets ID: 479594
Neospora caninum, hypothetical protein
Source Database / ID: 

pI: 9.1666 | Length (AA): 2056 | MW (Da): 216233 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
242 630 2c0t (A) 108 500 21.00 0 1 0.312302 0.36
274 616 5xzw (B) 107 466 34.00 0 1 0.346929 0.38
323 807 3v5w (A) 160 656 18.00 0 1 0.325295 0.41
331 616 3ag9 (B) 19 297 33.00 0 1 0.529205 -0.51
350 617 4c2v (A) 89 344 40.00 0 1 0.65145 -0.92
351 622 5l8j (A) 130 389 42.00 0 1 0.523696 -0.44
356 620 2rku (A) 44 309 33.00 0 1 0.590991 -0.73
368 732 4cfh (A) 22 443 21.00 0 1 0.353629 0.53
1466 1751 4wj1 (A) 4 286 10.00 0 0.01 0.161505 -0.05

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127024)

Species Accession Gene Product
Arabidopsis thaliana AT4G32830   serine/threonine-protein kinase aurora-1
Brugia malayi Bm1_12390   serine/threonine protein kinase 6
Brugia malayi Bm1_03120   serine/threonine kinase 12
Brugia malayi Bm1_15415   serine/threonine-protein kinase 6
Candida albicans CaO19.3474   likely protein kinase similar to S. cerevisiae IPL1 (YPL209C) Aurora protein kinase involved in regulating kinetochore-microtubu
Candida albicans CaO19.10978   likely protein kinase similar to S. cerevisiae IPL1 (YPL209C) Aurora protein kinase involved in regulating kinetochore-microtubu
Caenorhabditis elegans CELE_B0207.4   Protein AIR-2
Cryptosporidium hominis Chro.20335   protein kinase (EC 2.7.1.-) p46XlEg22
Cryptosporidium parvum cgd2_3190   protein kinase, putative
Dictyostelium discoideum DDB_G0279343   Aurora family protein kinase
Drosophila melanogaster Dmel_CG3068   aurora
Drosophila melanogaster Dmel_CG6620   IplI-aurora-like kinase
Echinococcus granulosus EgrG_001059700   aurora kinase A
Echinococcus granulosus EgrG_000891900   serine:threonine protein kinase 12 B
Entamoeba histolytica EHI_177010   serine/threonine- protein kinase 6 , putative
Entamoeba histolytica EHI_193840   protein kinase , putative
Entamoeba histolytica EHI_033850   serine/threonine- protein kinase 6, putative
Entamoeba histolytica EHI_011220   protein kinase, putative
Entamoeba histolytica EHI_071320   protein kinase, putative
Entamoeba histolytica EHI_104940   serine/threonine protein kinase 6, putative
Entamoeba histolytica EHI_142710   protein kinase domain containing protein
Echinococcus multilocularis EmuJ_001059700   aurora kinase A
Echinococcus multilocularis EmuJ_000891900   serine:threonine protein kinase 12 B
Giardia lamblia GL50803_5358   Aurora kinase
Homo sapiens ENSG00000178999   aurora kinase B
Homo sapiens ENSG00000087586   aurora kinase A
Homo sapiens ENSG00000105146   aurora kinase C
Leishmania braziliensis LbrM.28.0540   protein kinase, putative
Leishmania donovani LdBPK_280550.1   Aurora I-related kinase
Leishmania infantum LinJ.28.0550   protein kinase, putative
Leishmania major LmjF.28.0520   protein kinase, putative
Leishmania mexicana LmxM.28.0520   protein kinase, putative
Loa Loa (eye worm) LOAG_02905   AUR protein kinase
Loa Loa (eye worm) LOAG_01615   AUR protein kinase
Loa Loa (eye worm) LOAG_03125   AUR protein kinase
Mus musculus ENSMUSG00000027496   aurora kinase A
Mus musculus ENSMUSG00000070837   aurora kinase C
Mus musculus ENSMUSG00000020897   aurora kinase B
Neospora caninum NCLIV_021920   hypothetical protein
Oryza sativa 4327302   Os01g0191800
Plasmodium berghei PBANKA_0407400   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_0309200   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_0833500   serine/threonine protein kinase, putative
Plasmodium vivax PVX_119575   serine/threonine protein kinase 6, putative
Plasmodium yoelii PY03456   putative protein kinase
Plasmodium yoelii PY03956   Protein kinase domain, putative
Saccharomyces cerevisiae YPL209C   aurora kinase
Schistosoma japonicum Sjp_0046880   ko:K08850 aurora kinase, putative
Schistosoma japonicum Sjp_0093670   Serine/threonine-protein kinase 12, putative
Schistosoma mansoni Smp_162710   protein kinase
Schistosoma mansoni Smp_135360   serine/threonine protein kinase
Schmidtea mediterranea mk4.000743.05   Serine/threonine-protein kinase flr-4
Schmidtea mediterranea mk4.005195.01   Aurora kinase-2
Schmidtea mediterranea mk4.006705.00   Aurora/Ipl1-related protein kinase 1
Schmidtea mediterranea mk4.008584.00   EG:8D8.5 protein
Schmidtea mediterranea mk4.008598.01   Aurora kinase-1
Schmidtea mediterranea mk4.000916.10   Serine/threonine-protein kinase flr-4
Schmidtea mediterranea mk4.014531.00   Aurora kinase-2
Schmidtea mediterranea mk4.006705.01   Protein kinase
Schmidtea mediterranea mk4.034612.00   Aurora/IPL1-related protein kinase 2
Schmidtea mediterranea mk4.005195.02   Aurora/IPL1-related protein kinase 2
Schmidtea mediterranea mk4.011981.00   Aurora/IPL1-related protein kinase 2
Schmidtea mediterranea mk4.016831.00  
Trypanosoma brucei gambiense Tbg972.11.9400   protein kinase, putative
Trypanosoma brucei Tb927.11.8220   aurora B kinase
Trypanosoma congolense TcIL3000.11.8760   aurora B kinase
Trypanosoma cruzi TcCLB.508817.80   aurora B kinase, putative
Toxoplasma gondii TGME49_203010   aurora kinase
Trichomonas vaginalis TVAG_300450   AGC family protein kinase
Trichomonas vaginalis TVAG_030770   AGC family protein kinase
Trichomonas vaginalis TVAG_475150   AGC family protein kinase
Trichomonas vaginalis TVAG_050080   AGC family protein kinase

Essentiality

NCLIV_021920 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.0330 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.0330 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.0330 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.0330 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0207.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0207.4 Caenorhabditis elegans sterile wormbase
YPL209C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0407400 Plasmodium berghei Essential plasmo
TGME49_203010 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Xenopus laevis Aurora kinase B-A Compounds References
Xenopus laevis Aurora kinase B-B Compounds References
Mus musculus aurora kinase B Compounds References
Homo sapiens aurora kinase B Compounds References
Mus musculus aurora kinase A Compounds References
Homo sapiens aurora kinase A Compounds References
Homo sapiens aurora kinase C Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 28.4% 289 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 22.4% 196 aa Compounds References
Patiria pectinifera Cdc2 300 aa 29.4% 282 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 25.9% 290 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 19.9% 292 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 32.4% 296 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 33.1% 296 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 26.2% 294 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 32.5% 160 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 29.0% 283 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier NCLIV_021920 (Neospora caninum), hypothetical protein
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