Detailed view for TcCLB.508357.80

Basic information

TDR Targets ID: 48304
Trypanosoma cruzi, cation transporter protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6568 | Length (AA): 608 | MW (Da): 67287 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 15

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01545   Cation efflux family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0008324   cation transmembrane transporter activity  
GO:0055085   transmembrane transport  
GO:0006812   cation transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
54 139 5hn8 (A) 235 344 35.00 0.98 0.04 0.128947 1.93
114 476 3rko (L) 141 472 28.00 0.74 0.36 0.571539 0.78
527 607 2zzt (A) 213 289 8.00 0.15 0.09 0.225224 -0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127157)

Species Accession Gene Product
Arabidopsis thaliana AT3G61940   zinc transporter (ZAT) family protein
Arabidopsis thaliana AT2G04620   cation efflux family protein
Brugia malayi Bm1_01670   cation efflux family protein
Candida albicans CaO19.10644   likely cation efflux pump similar to S. cerevisiae nuclear cation:cation antiporter MSC2 (YDR205W) involved in zinc ion homeosta
Candida albicans CaO19.3132   likely cation efflux pump similar to S. cerevisiae nuclear cation:cation antiporter MSC2 (YDR205W) involved in zinc ion homeosta
Caenorhabditis elegans CELE_Y105E8A.3   Protein Y105E8A.3
Dictyostelium discoideum DDB_G0291141   cation diffusion facilitator family protein
Drosophila melanogaster Dmel_CG6672   Zinc transporter 86D
Escherichia coli b0752   zinc efflux system
Echinococcus granulosus EgrG_001002800   cation efflux protein: zinc transporter
Echinococcus granulosus EgrG_001068700   zinc transporter 5
Entamoeba histolytica EHI_026390   cation transporter, putative
Echinococcus multilocularis EmuJ_001068700   zinc transporter 5
Echinococcus multilocularis EmuJ_001002800   cation efflux protein: zinc transporter
Homo sapiens ENSG00000164756   solute carrier family 30 (zinc transporter), member 8
Homo sapiens ENSG00000162695   solute carrier family 30 (zinc transporter), member 7
Homo sapiens ENSG00000145740   solute carrier family 30 (zinc transporter), member 5
Leishmania braziliensis LbrM.27.1380   cation transporter, putative
Leishmania donovani LdBPK_271160.1   cation transporter, putative
Leishmania infantum LinJ.27.1160   cation transporter, putative
Leishmania major LmjF.27.1265   cation transporter, putative
Leishmania mexicana LmxM.27.1265   cation transporter, putative
Loa Loa (eye worm) LOAG_09039   hypothetical protein
Mycobacterium leprae ML0283   Possible cation-efflux transporter component
Mus musculus ENSMUSG00000021629   solute carrier family 30 (zinc transporter), member 5
Mus musculus ENSMUSG00000022315   solute carrier family 30 (zinc transporter), member 8
Mus musculus ENSMUSG00000054414   solute carrier family 30 (zinc transporter), member 7
Mycobacterium ulcerans MUL_2837   efflux membrane protein
Oryza sativa 4345598   Os08g0422200
Onchocerca volvulus OVOC1764   Zinc transporter homolog
Saccharomyces cerevisiae YDR205W   metal cation transporter MSC2
Schistosoma japonicum Sjp_0130960   hypothetical protein
Schistosoma japonicum Sjp_0089340   Zinc transporter 5, putative
Schistosoma japonicum Sjp_0089360   similar to Centrosomal protein of 152 kDa, putative
Schistosoma mansoni Smp_170590   cation efflux protein/ zinc transporter
Schmidtea mediterranea mk4.017357.01  
Schmidtea mediterranea mk4.001527.06   Putative cation efflux protein/ zinc transporter
Trypanosoma brucei gambiense Tbg972.11.2040   cation transporter protein, putative
Trypanosoma brucei Tb927.11.1910   cation transporter protein, putative
Trypanosoma brucei Tb427tmp.42.0002   cation transporter protein, putative
Trypanosoma congolense TcIL3000_0_39100   cation transporter protein, putative
Trypanosoma cruzi TcCLB.508357.80   cation transporter protein, putative
Trypanosoma cruzi TcCLB.508355.420   cation transporter protein, putative
Trichomonas vaginalis TVAG_285720   cation efflux protein/ zinc transporter, putative
Trichomonas vaginalis TVAG_132220   cation efflux protein/ zinc transporter, putative
Trichomonas vaginalis TVAG_228020   cation efflux protein/ zinc transporter, putative
Trichomonas vaginalis TVAG_524900   cation efflux protein/ zinc transporter, putative
Wolbachia endosymbiont of Brugia malayi Wbm0383   Co/Zn/Cd efflux system component

Essentiality

TcCLB.508357.80 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.42.0002 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.42.0002 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.42.0002 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.42.0002 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0752 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.508357.80 (Trypanosoma cruzi), cation transporter protein, putative
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