Detailed view for TcCLB.508173.170

Basic information

TDR Targets ID: 49174
Trypanosoma cruzi, Nuclear Dbf2-related kinase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8149 | Length (AA): 432 | MW (Da): 49638 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 412 2vd5 (A) 11 396 31.00 0 1 1.09243 0.52
25 384 2vd5 (A) 33 356 44.00 0 1 1.15433 0.15
65 213 3cok (A) 9 154 21.00 0 0.19 0.448207 0.27
191 324 4o38 (A) 170 289 45.00 0.079 0.44 -0.206815 2.37
291 364 4btf (A) 380 452 18.00 0 1 0.429596 -0.98

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126822)

Species Accession Gene Product
Arabidopsis thaliana AT2G20470   AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein
Arabidopsis thaliana AT2G19400   AGC kinase family protein
Arabidopsis thaliana AT3G23310   AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein
Arabidopsis thaliana AT4G33080   AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein
Arabidopsis thaliana AT1G30640   protein kinase family protein
Arabidopsis thaliana AT5G09890   Protein kinase family protein
Arabidopsis thaliana AT1G03920   putative serine/threonine protein kinase
Arabidopsis thaliana AT4G14350   AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein
Brugia malayi Bm1_29575   Protein kinase domain containing protein
Candida albicans CaO19.4909   similar to S. cerevisiae cell wall biosynthesis kinase, nuclear protein kinase involved in morphogenesis during mating
Candida albicans CaO19.12375   similar to S. cerevisiae cell wall biosynthesis kinase, nuclear protein kinase involved in morphogenesis during mating
Caenorhabditis elegans CELE_T20F10.1   Protein WTS-1
Caenorhabditis elegans CELE_R11G1.4   Protein SAX-1, isoform B
Cryptosporidium hominis Chro.10143   CBK1
Cryptosporidium hominis Chro.20198   hypothetical protein
Cryptosporidium parvum cgd2_1830   hypothetical protein
Dictyostelium discoideum DDB_G0288753   NDR family protein kinase
Dictyostelium discoideum DDB_G0278457   NDR family protein kinase
Drosophila melanogaster Dmel_CG8637   tricornered
Drosophila melanogaster Dmel_CG12072   warts
Echinococcus granulosus EgrG_000896700   NDR protein kinase
Echinococcus granulosus EgrG_000700100   NDR protein kinase
Entamoeba histolytica EHI_002790   protein kinase, putative
Entamoeba histolytica EHI_068600   protein kinase, putative
Entamoeba histolytica EHI_110490   protein kinase, putative
Echinococcus multilocularis EmuJ_000700100   serine:threonine protein kinase LATS2
Echinococcus multilocularis EmuJ_000896700   serine:threonine protein kinase 38
Giardia lamblia GL50803_8587   Kinase, AGC NDR
Homo sapiens ENSG00000150457   large tumor suppressor kinase 2
Homo sapiens ENSG00000112079   serine/threonine kinase 38
Homo sapiens ENSG00000211455   serine/threonine kinase 38 like
Homo sapiens ENSG00000131023   large tumor suppressor kinase 1
Leishmania braziliensis LbrM.06.1160   protein kinase, putative
Leishmania donovani LdBPK_061220.1   protein kinase, putative
Leishmania infantum LinJ.06.1220   protein kinase, putative
Leishmania major LmjF.06.1180   protein kinase, putative
Leishmania mexicana LmxM.06.1180   protein kinase, putative
Loa Loa (eye worm) LOAG_00928   AGC/NDR protein kinase
Loa Loa (eye worm) LOAG_07025   AGC/NDR protein kinase
Mus musculus ENSMUSG00000024006   serine/threonine kinase 38
Mus musculus ENSMUSG00000040021   large tumor suppressor
Mus musculus ensembl-mmu:ENSMUSG00000001630   serine/threonine kinase 38 like
Mus musculus ENSMUSG00000021959   large tumor suppressor 2
Oryza sativa 9271850   Os12g0290100
Oryza sativa 4339288   Os05g0511400
Oryza sativa 4348888   Os10g0476100
Oryza sativa 4325661   Os01g0186700
Saccharomyces cerevisiae YNL161W   Cbk1p
Schistosoma japonicum Sjp_0203020   Serine/threonine-protein kinase 38-like, putative
Schistosoma japonicum Sjp_0203030   Serine/threonine-protein kinase 38-like, putative
Schistosoma mansoni Smp_103570   serine/threonine protein kinase
Schistosoma mansoni Smp_171920   serine/threonine protein kinase
Schmidtea mediterranea mk4.000628.06  
Schmidtea mediterranea mk4.002603.02  
Schmidtea mediterranea mk4.001772.03  
Schmidtea mediterranea mk4.003800.01   Serine/threonine-protein kinase tricorner
Schmidtea mediterranea mk4.003789.01   Serine/threonine-protein kinase tricorner
Trypanosoma brucei gambiense Tbg972.7.6700   protein kinase, putative
Trypanosoma brucei Tb927.7.5770   Nuclear Dbf2-related kinase
Trypanosoma congolense TcIL3000_7_4740   protein kinase, putative
Trypanosoma cruzi TcCLB.508173.170   Nuclear Dbf2-related kinase
Trichomonas vaginalis TVAG_009500   AGC family protein kinase
Trichomonas vaginalis TVAG_378200   AGC family protein kinase
Trichomonas vaginalis TVAG_508070   AGC family protein kinase
Trichomonas vaginalis TVAG_400740   AGC family protein kinase
Trichomonas vaginalis TVAG_036320   AGC family protein kinase
Trichomonas vaginalis TVAG_118330   AGC family protein kinase
Trichomonas vaginalis TVAG_310280   AGC family protein kinase

Essentiality

TcCLB.508173.170 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.5770 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.5770 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.5770 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T20F10.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20F10.1 Caenorhabditis elegans larval lethal wormbase
CELE_T20F10.1 Caenorhabditis elegans slow growth wormbase
YNL161W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens serine/threonine kinase 38 like Compounds References
Homo sapiens large tumor suppressor kinase 2 Compounds References
Homo sapiens serine/threonine kinase 38 Compounds References
Homo sapiens large tumor suppressor kinase 1 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 20.0% 305 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 28.3% 381 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 30.4% 352 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 22.8% 337 aa Compounds References
Patiria pectinifera Cdc2 300 aa 26.1% 261 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 22.9% 328 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 25.2% 309 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 23.1% 337 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 24.9% 169 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 25.3% 308 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 29.1% 381 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0056 1 0.5
0.0081 1 0.5
0.0088 0.4477 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0059 1 1
0.0022 0.5 0.5
0.0063 0.7244 0.2543
0.0064 0.3377 0
0.0016 0.5 0.5
0.0033 1 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0037 1 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0063 1 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0008 0.5 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0098 0.3242 0.3649
0.0039 0.9485 0.5
0.0059 1 1
0.0062 0.6935 0
0.0066 0.3101 0
0.0027 1 0.5
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0036 0.5 0.5
0.0026 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.508173.170 (Trypanosoma cruzi), Nuclear Dbf2-related kinase
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