Detailed view for PF3D7_0924300

Basic information

TDR Targets ID: 4918
Plasmodium falciparum, thiamine pyrophosphokinase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.8717 | Length (AA): 400 | MW (Da): 46709 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04263   Thiamin pyrophosphokinase, catalytic domain
PF04265   Thiamin pyrophosphokinase, vitamin B1 binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0030975   thiamin binding  
GO:0020011   apicoplast  
GO:0005524   ATP binding  
GO:0004788   thiamin diphosphokinase activity  
GO:0009229   thiamin diphosphate biosynthetic process  
GO:0006772   thiamin metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
60 387 1ig3 (A) 10 263 31.00 0 1 0.76 0.03
88 366 2g9z (A) 52 304 32.00 0.000000000039 0.92 0.77 0.74
171 387 1ig3 (A) 40 263 31.00 0 1 0.85 -0.86
82 397 1ig3 (A) 26 263 35.00 0 1 0.76 0.79
176 397 1ig3 (B) 36 263 29.00 0 1 0.8802 -0.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, Oocyst. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 48 hs, gametocyte, early schizont, late trophozoite, Female Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 24 hs, late schizont, Ring, Sporozoite, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 16 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_128213)

Species Accession Gene Product
Arabidopsis thaliana AT1G02880   thiamin pyrophosphokinase1
Arabidopsis thaliana AT2G44750   thiamin pyrophosphokinase 2
Brugia malayi Bm1_43600   Thiamin pyrophosphokinase, catalytic domain containing protein
Candida albicans CaO19.1110   thiamine pyrophosphokinase
Candida albicans CaO19.8707   thiamine pyrophosphokinase
Caenorhabditis elegans CELE_ZK637.9   Protein TPK-1, isoform B
Dictyostelium discoideum DDB_G0267546   hypothetical protein
Drosophila melanogaster Dmel_CG14721   CG14721 gene product from transcript CG14721-RA
Echinococcus granulosus EgrG_000831900   thiamin pyrophosphokinase 1
Entamoeba histolytica EHI_148290   thiamine pyrophosphokinase, putative
Echinococcus multilocularis EmuJ_000831900   thiamin pyrophosphokinase 1
Giardia lamblia GL50803_11763   hypothetical protein
Homo sapiens ENSG00000196511   thiamin pyrophosphokinase 1
Loa Loa (eye worm) LOAG_11448   thiamin pyrophosphokinase
Mus musculus ENSMUSG00000029735   thiamine pyrophosphokinase
Neospora caninum NCLIV_052210   hypothetical protein
Oryza sativa 4326818   Os01g0931400
Oryza sativa 4338566   Os05g0367400
Oryza sativa 4326288   Os01g0356500
Plasmodium berghei PBANKA_0825200   thiamine pyrophosphokinase, putative
Plasmodium falciparum PF3D7_0924300   thiamine pyrophosphokinase
Plasmodium knowlesi PKNH_0722300   thiamine pyrophosphokinase, putative
Plasmodium vivax PVX_099630   thiamine pyrophosphokinase, putative
Plasmodium yoelii PY05196   Arabidopsis thaliana At1g02880/F22D16_33, putative
Saccharomyces cerevisiae YOR143C   thiamine diphosphokinase
Schistosoma japonicum Sjp_0020260   ko:K00949 thiamine pyrophosphokinase [EC2.7.6.2], putative
Schistosoma mansoni Smp_032640   thiamin pyrophosphokinase
Toxoplasma gondii TGME49_215250   thiamin pyrophosphokinase, catalytic domain-containing protein
Treponema pallidum TP0518   hypothetical protein
Trichomonas vaginalis TVAG_096630   thiamin pyrophosphokinase, putative
Trichomonas vaginalis TVAG_049690   thiamin pyrophosphokinase, putative
Trichomonas vaginalis TVAG_256470   thiamin pyrophosphokinase, putative

Essentiality

PF3D7_0924300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
YOR143C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0825200 Plasmodium berghei Dispensable plasmo
TGME49_215250 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Pviv008849AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Pviv008849; Recombinant protein: full-length; Source: P vivax; hypothetical protein homolog ; Identifier: Pv099630

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier PF3D7_0924300 (Plasmodium falciparum), thiamine pyrophosphokinase
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