Detailed view for PF3D7_0704500

Basic information

TDR Targets ID: 495
Plasmodium falciparum, serine/threonine protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.7315 | Length (AA): 1603 | MW (Da): 192854 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
908 948 1bu1 (A) 95 137 20.00 0.00002 0.01 0.11 -0.1
1067 1397 1pmn (A) 51 389 17.00 0 1 0.38 -0.23
1078 1228 2bfx (A) 91 240 21.00 0 0.98 0.49 -1.43
1071 1511 3q5i (A) 48 507 21.00 0 1 0.370909 0.78
1072 1280 3b2t (A) 473 698 17.00 0.037 1 0.189681 -0.18
1078 1273 3dj6 (A) 144 336 18.00 0.00000059 1 0.240571 -0.47
1094 1226 2x7f (D) 39 175 33.00 0.032 0.86 0.296069 0.47
976 1351 4ejn (A) 13 431 15.00 0 1 0.294881 0.64
1057 1269 3bkb (A) 553 755 21.00 0.0077 1 0.226494 -0.03
1060 1500 3q5i (A) 48 507 20.00 0 0.92 0.38671 0.65
1067 1262 3dj6 (A) 144 336 18.00 0.00000054 1 0.259816 -0.47
1083 1215 2x7f (D) 39 175 33.00 0.031 0.86 0.296743 0.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 4MVF:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, early schizont, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 32 hs, sporozoite, early ring, late trophozoite, Oocyst, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 16 hs, early trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 24 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_167124)

Species Accession Gene Product
Plasmodium berghei PBANKA_0802200   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_0704500   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_0103000   calcium-dependent protein kinase 3, putative
Plasmodium vivax PVX_087765   calcium-dependent protein kinase 3, putative
Plasmodium yoelii PY01945   serine/threonine-protein kinase, putative

Essentiality

PF3D7_0704500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0802200 Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Sus scrofa Casein kinase I isoform alpha 125 aa 25.8% 124 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0088 0.4477 0.5
0.0091 1 0.5
0.0067 0.5 0.5
0.0012 0.5 0.5
0.0059 1 1
0.0081 0.5 0.5
0.0027 1 0.5
0.0016 0.5 0.5
0.0093 0.8828 0
0.0012 0.5 0.5
0.0022 0.5 0.5
0.0081 1 0.5
0.0059 1 1
0.0018 0.5 0.5
0.0026 0.5 0.5
0.0056 1 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0029 0.5 0.5
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0069 0.3067 1
0.0039 0.5 0.5
0.0092 1 0.5
0.0063 0.7244 0.7244
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0066 0.3101 0
0.0011 1 0.5
0.0008 0.5 0.5
0.0064 0.3377 0
0.0042 0.5 0.5
0.0032 0.5 0.5
0.0061 0.6883 1
0.0062 0.6935 0
0.0033 1 1
0.0032 0.5 0.5
0.0007 0.5 0.5
0.0039 0.9485 0.5
0.0059 1 1
0.0003 0.5 0.5
0.0036 0.5 0.5
0.0004 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0704500 (Plasmodium falciparum), serine/threonine protein kinase, putative
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