Detailed view for PF3D7_0928800

Basic information

TDR Targets ID: 4962
Plasmodium falciparum, serine/threonine protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 10.1223 | Length (AA): 820 | MW (Da): 99169 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
516 818 1yhv (A) 265 522 19.00 0 1 0.4 0.6
598 816 2bik (B) 117 289 23.00 0.000000000022 0.99 0.34 -0.34
278 453 4uos (A) 2 179 16.00 0.5 0.18 0.338434 -0.47
493 817 3ofm (A) 12 325 16.00 0 1 0.276441 0.93
516 637 4uos (A) 1 137 39.00 0.0048 0.96 0.36328 0.39
567 817 3fbv (A) 713 980 19.00 0.000000000017 0.91 0.345898 0.6
365 550 4tql (A) 44 234 18.00 0.58 0.26 0.37091 -0.2
498 799 4b8l (A) 89 344 20.00 0 1 0.161229 1.09
507 603 4uos (A) 75 181 42.00 0.041 0.78 0.402599 0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile gametocyte, sporozoite, early schizont, early trophozoite, late trophozoite, Sporozoite, Female Gametocyte, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, late ring, late schizont, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, Ring. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_167160)

Species Accession Gene Product
Plasmodium berghei PBANKA_0829600   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_0928800   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_0727100   serine/threonine protein kinase, putative
Plasmodium vivax PVX_099890   serine/threonine protein kinase, putative
Plasmodium yoelii PY04175   hypothetical protein

Essentiality

PF3D7_0928800 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0829600 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 16.5% 272 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 21.1% 298 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.7244
0.0064 0.3377 0
0.0056 1 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0066 0.3101 0
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0027 1 0.5
0.0061 0.6883 1
0.0039 0.5 0.5
0.0036 0.5 0.5
0.0026 0.5 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0062 0.6935 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_0928800 (Plasmodium falciparum), serine/threonine protein kinase, putative
Title for this comment
Comment