Detailed view for TcCLB.503463.30

Basic information

TDR Targets ID: 49697
Trypanosoma cruzi, CMGC/SRPK protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.5349 | Length (AA): 874 | MW (Da): 97091 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
287 804 1fmk () 82 519 15.00 0 0.7 0.42 0.77
425 865 1ymi (A) 4 324 20.00 0 1 0.35 0.42
448 867 1wak (A) 64 655 33.00 0 1 0.71 -0.49
443 610 3dls (A) 989 1135 32.00 0.15 1 0.22802 1.32
446 866 5my8 (A) 64 654 37.00 0 1 0.681493 -0.02
448 873 2eu9 (A) 142 480 30.00 0 1 0.543214 0.42
455 866 2x7g (A) 85 698 37.00 0 1 0.663196 0.06
458 791 3bhy (A) 9 262 30.00 0.066 1 0.273951 0.76
460 821 2w9z (B) 4 284 33.00 0.0000019 1 0.255988 1.06
461 638 3dfa (A) 6 172 30.00 0.0044 0.83 0.388461 0.26
468 610 4fg7 (A) 26 148 37.00 0.037 0.27 0.112416 1.36
560 615 2hw6 (A) 131 182 33.00 0.42 0.88 0.404873 -0.15
706 792 4i5p (A) 237 323 22.00 0.39 0.14 0.349342 -0.38
712 746 3e3p (A) 189 224 49.00 0.068 0.15 0.421846 0.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_134553)

Species Accession Gene Product
Leishmania braziliensis LbrM.26.0990   protein kinase, putative
Leishmania donovani LdBPK_260950.1   protein kinase, putative
Leishmania infantum LinJ.26.0950   protein kinase, putative
Leishmania major LmjF.26.0980   protein kinase, putative
Leishmania mexicana LmxM.26.0980   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.7.910   protein kinase, putative
Trypanosoma brucei Tb927.7.960   CMGC/SRPK protein kinase, putative
Trypanosoma congolense TcIL3000_7_600   protein kinase, putative
Trypanosoma cruzi TcCLB.503463.30   CMGC/SRPK protein kinase, putative
Trypanosoma cruzi TcCLB.508215.3   CMGC/SRPK protein kinase, putative
Trypanosoma cruzi TcCLB.477079.10   protein kinase, putative
Trichomonas vaginalis TVAG_450330   CMGC family protein kinase
Trichomonas vaginalis TVAG_066370   CMGC family protein kinase

Essentiality

TcCLB.503463.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.960 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.960 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 21.5% 284 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0007 0.5 0.5
0.0016 0.5 0.5
0.0033 1 0.5
0.0012 0.5 0.5
0.0064 0.3377 0
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.2543
0.0093 0.8828 0
0.0032 0.5 0.5
0.0056 1 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0036 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0059 1 1
0.0062 0.6935 0
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.3649
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 0.5
0.0033 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0018 0.5 0.5
0.0042 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.503463.30 (Trypanosoma cruzi), CMGC/SRPK protein kinase, putative
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