Detailed view for TcCLB.509633.10

Basic information

TDR Targets ID: 49815
Trypanosoma cruzi, CBF/Mak21 family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9222 | Length (AA): 336 | MW (Da): 37277 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03914   CBF/Mak21 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0042254   ribosome biogenesis and assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
199 335 2c0g (A) 1070 1196 29.00 0.31 0.02 0.475338 1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128560)

Species Accession Gene Product
Arabidopsis thaliana AT2G17250   protein NUCLEOLAR COMPLEX ASSOCIATED 4
Babesia bovis BBOV_II005130   hypothetical protein
Brugia malayi Bm1_46420   Hypothetical 58.5 kDa protein T20B12.3 in chromosome III
Candida albicans CaO19.9458   similar to essential nucleolar protein
Candida albicans CaO19.1902   similar to essential nucleolar protein
Caenorhabditis elegans CELE_T20B12.3   Protein T20B12.3
Dictyostelium discoideum DDB_G0275403   hypothetical protein
Drosophila melanogaster Dmel_CG2875   CG2875 gene product from transcript CG2875-RC
Echinococcus granulosus EgrG_000535800   nucleolar complex protein 4
Entamoeba histolytica EHI_083570   nuclear complex protein 4, putative
Entamoeba histolytica EHI_070740   CBF/Mak21 family
Echinococcus multilocularis EmuJ_000535800   nucleolar complex protein 4
Homo sapiens ENSG00000184967   nucleolar complex associated 4 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.20.0670   hypothetical protein, conserved
Leishmania donovani LdBPK_340780.1   CBF/Mak21 family, putative
Leishmania infantum LinJ.34.0780   hypothetical protein, conserved
Leishmania major LmjF.34.0740   hypothetical protein, conserved
Leishmania mexicana LmxM.33.0740   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_01903   hypothetical protein
Mus musculus ENSMUSG00000033294   nucleolar complex associated 4 homolog (S. cerevisiae)
Neospora caninum NCLIV_010500   hypothetical protein
Oryza sativa 4336790   Os04g0585300
Onchocerca volvulus OVOC8856   Nucleolar complex protein 4 homolog
Saccharomyces cerevisiae YPR144C   Noc4p
Schistosoma japonicum Sjp_0007010   similar to Uncharacterized protein C1604.06c, putative
Schistosoma mansoni Smp_017640   nucleolar complex protein
Trypanosoma brucei gambiense Tbg972.4.3630   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.3670   CBF/Mak21 family, putative
Trypanosoma congolense TcIL3000_4_3170   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506201.110   CBF/Mak21 family, putative
Trypanosoma cruzi TcCLB.509633.10   CBF/Mak21 family, putative
Toxoplasma gondii TGME49_319662   histone lysine methyltransferase, SET, putative
Theileria parva TP04_0054   hypothetical protein
Trichomonas vaginalis TVAG_160960   conserved hypothetical protein

Essentiality

TcCLB.509633.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.3670 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.3670 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.3670 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T20B12.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20B12.3 Caenorhabditis elegans larval arrest wormbase
CELE_T20B12.3 Caenorhabditis elegans slow growth wormbase
CELE_T20B12.3 Caenorhabditis elegans sterile wormbase
YPR144C Saccharomyces cerevisiae inviable yeastgenome
TGME49_319662 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.509633.10 (Trypanosoma cruzi), CBF/Mak21 family, putative
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