Detailed view for TcCLB.503999.20

Basic information

TDR Targets ID: 50020
Trypanosoma cruzi, Kinesin-13 5, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.3484 | Length (AA): 724 | MW (Da): 80516 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00225   Kinesin motor domain
PF00536   SAM domain (Sterile alpha motif)

Gene Ontology

Mouse over links to read term descriptions.
GO:0008017   microtubule binding  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0003777   microtubule motor activity  
GO:0007018   microtubule-based movement  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
124 501 1n6m (B) 299 668 30.00 0 1 0.56 0.09
180 496 1v8k (A) 69 395 53.00 0 1 1.01 -1.18
183 537 1sdm (A) 889 1252 33.00 0 1 0.73 -0.75
183 501 2h58 (A) 445 766 37.00 0 1 0.9 -1.19
2 64 5j8y (C) 1142 1203 18.00 0 0.75 0.501817 -1.99
2 62 2k4p (A) 26 86 25.00 0 1 0.552054 -1.41
3 61 4nl9 (A) 9 66 31.00 0.31 0.89 0.598292 -1.66
4 69 1b0x (A) 915 980 17.00 0.0013 1 0.55096 -2.28
183 533 5mio (C) 258 646 55.00 0 1 1.14361 -0.8
656 723 5j2l (B) 4 71 9.00 0.51 0 0.240723 -0.95

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127200)

Species Accession Gene Product
Arabidopsis thaliana AT3G16630   kinesin 13A
Arabidopsis thaliana AT3G16060   ATP binding microtubule motor family protein
Babesia bovis BBOV_II007750   kinesin-like protein, putative
Brugia malayi Bm1_42920   Kinesin motor domain containing protein
Caenorhabditis elegans CELE_K11D9.1   Protein KLP-7, isoform B
Cryptosporidium hominis Chro.80383   kinesin-like protein
Cryptosporidium parvum cgd8_3300   centromere associated Kip3p, kinesin like P-loop NTpase that belongs to the TRAFAC class GTpase superfamily
Dictyostelium discoideum DDB_G0267404   SAM domain-containing protein
Drosophila melanogaster Dmel_CG1453   Kinesin-like protein at 10A
Echinococcus granulosus EgrG_001159400   kinesin protein KIF2A
Echinococcus granulosus EgrG_001118400   kinesin protein KIF2A
Echinococcus multilocularis EmuJ_001118400   kinesin protein KIF2A
Echinococcus multilocularis EmuJ_001159400   kinesin protein KIF2A
Giardia lamblia GL50803_16945   Kinesin-13
Homo sapiens ENSG00000186638   kinesin family member 24
Homo sapiens 3796   kinesin heavy chain member 2A
Homo sapiens ENSG00000142945   kinesin family member 2C
Homo sapiens ENSG00000141200   kinesin family member 2B
Leishmania braziliensis LbrM.01.0060   MCAK-like kinesin, putative
Leishmania braziliensis LbrM.13.1470   MCAK-like kinesin, putative
Leishmania donovani LdBPK_010030.1   Kinesin-13 1, putative
Leishmania donovani LdBPK_131350.1   Kinesin-13 5, putative
Leishmania infantum LinJ.01.0030   MCAK-like kinesin, putative
Leishmania infantum LinJ.13.1350   MCAK-like kinesin, putative
Leishmania major LmjF.13.1610   MCAK-like kinesin, putative
Leishmania major LmjF.01.0030   MCAK-like kinesin, putative
Leishmania mexicana LmxM.01.0030   MCAK-like kinesin, putative
Leishmania mexicana LmxM.13.1610   MCAK-like kinesin, putative
Mus musculus ENSMUSG00000046755   kinesin family member 2B
Mus musculus ENSMUSG00000028678   kinesin family member 2C
Mus musculus ENSMUSG00000028438   kinesin family member 24
Mus musculus ENSMUSG00000021693   kinesin family member 2A
Neospora caninum NCLIV_013810   hypothetical protein
Oryza sativa 4337845   Os05g0154700
Oryza sativa 4326684   Os01g0625200
Plasmodium berghei PBANKA_1458300   kinesin-13, putative
Plasmodium falciparum PF3D7_1245100   kinesin-13, putative
Plasmodium knowlesi PKNH_1464800   kinesin-13, putative
Plasmodium vivax PVX_101150   kinesin-13, putative
Plasmodium yoelii PY07317   kinesin homolog-related
Plasmodium yoelii PY03174   kinesin-like protein K6
Schistosoma japonicum Sjp_0022180   ko:K10393 kinesin family member 2/24, putative
Schistosoma mansoni Smp_002280   hypothetical protein
Schmidtea mediterranea mk4.000194.14   Kinesin like protein
Schmidtea mediterranea mk4.005044.01   Kinesin-like protein Klp10A
Schmidtea mediterranea mk4.000194.13   Kinesin-like protein Klp10A
Schmidtea mediterranea mk4.000194.10  
Schmidtea mediterranea mk4.000194.12   Kinesin-like protein Klp10A
Schmidtea mediterranea mk4.003054.02   Kinesin-like protein Klp10A
Schmidtea mediterranea mk4.000194.11  
Schmidtea mediterranea mk4.005044.03   Kinesin like protein
Schmidtea mediterranea mk4.003054.05   Kinesin like protein
Schmidtea mediterranea mk4.003054.01  
Trypanosoma brucei gambiense Tbg972.11.3650   MCAK-like kinesin, putative
Trypanosoma brucei gambiense Tbg972.9.1890   MCAK-like kinesin, putative
Trypanosoma brucei Tb927.9.3650   Kinesin-13 1
Trypanosoma brucei Tb927.11.3280   Kinesin-13 5, putative
Trypanosoma congolense TcIL3000_9_1140   Kinesin-13 1, putative
Trypanosoma congolense TcIL3000.11.3100   Kinesin-13 5, putative
Trypanosoma cruzi TcCLB.509589.30   Kinesin-13 5, putative
Trypanosoma cruzi TcCLB.503999.20   Kinesin-13 5, putative
Trypanosoma cruzi TcCLB.506895.40   Kinesin-13 1, putative
Trypanosoma cruzi TcCLB.508501.290   Kinesin-13 1, putative
Toxoplasma gondii TGME49_287160   internal kinesin motor domain protein
Theileria parva TP02_0685   kinesin, putative

Essentiality

TcCLB.503999.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0790 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.0790 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.0790 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0790 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.160.2260 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.2260 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.2260 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb09.160.2260 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_K11D9.1 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1458300 Plasmodium berghei Essential plasmo
TGME49_287160 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.503999.20 (Trypanosoma cruzi), Kinesin-13 5, putative
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