Detailed view for PF3D7_0933500

Basic information

TDR Targets ID: 5005
Plasmodium falciparum, gamma-tubulin complex component, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.2769 | Length (AA): 1512 | MW (Da): 180493 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04130   Spc97 / Spc98 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0043015   gamma-tubulin binding  
GO:0007020   microtubule nucleation  
GO:0005815   microtubule organizing center  
GO:0000922   spindle pole  
GO:0000226   microtubule cytoskeleton organization and biogenesis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 283 4o87 (A) 72 316 31.00 0.039 0.51 0.207369 1.01
337 505 4u5a (A) 53 238 19.00 0.65 0.12 0.286372 -0.51
553 1304 3rip (A) 1 654 17.00 0 0.82 0.184954 1.95
984 1273 3rip (A) 324 641 21.00 0 1 0.452399 -0.49
1220 1421 4tql (A) 32 239 13.00 0.61 0.03 0.210198 -0.63
1 282 4o87 (A) 72 316 30.00 0.04 0.39 0.244979 0.91
5 157 4u5a (C) 60 239 22.00 0.58 0.03 0.227492 -0.03
48 517 4h5y (A) 124 583 17.00 0.18 0.7 0.363565 0.88
421 1190 3s5h (A) 143 990 22.00 0.24 0.99 0.493372 1.46
602 736 4d10 (B) 38 197 31.00 0.09 0.69 0.337563 0.11
1206 1374 4zbw (A) 16 183 13.00 0.0000013 0.01 0.271995 -0.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, Oocyst, Ring, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs. Otto TD
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_128216)

Species Accession Gene Product
Arabidopsis thaliana AT5G06680   spindle pole body component 98
Candida albicans CaO19.10131   Spindle pole body component
Candida albicans CaO19.2600   Spindle pole body component
Cryptosporidium hominis Chro.60394   hypothetical protein
Cryptosporidium parvum cgd6_3410   tubulin, gamma tubulin complex protein 3
Dictyostelium discoideum DDB_G0283909   spindle pole body component 98
Drosophila melanogaster Dmel_CG10988   Gamma-tubulin ring protein 91
Echinococcus granulosus EgrG_000576100   gamma tubulin complex component 3
Entamoeba histolytica EHI_103790   tubulin interacting protein, putative
Echinococcus multilocularis EmuJ_000576100   gamma tubulin complex component 3
Giardia lamblia GL50803_12057   Gamma tubulin ring complex
Homo sapiens ENSG00000126216   tubulin, gamma complex associated protein 3
Leishmania braziliensis LbrM.28.2960   gamma-tubulin complex subunit, putative
Leishmania donovani LdBPK_282980.1   gamma-tubulin complex component 3
Leishmania infantum LinJ.28.2980   gamma-tubulin complex subunit, putative
Leishmania major LmjF.28.2760   gamma-tubulin complex subunit, putative
Leishmania mexicana LmxM.28.2760   gamma-tubulin complex subunit, putative
Mus musculus ENSMUSG00000000759   tubulin, gamma complex associated protein 3
Neospora caninum NCLIV_054230   Spc97 / Spc98 family domain-containing protein, putative
Oryza sativa 4347202   Os09g0446200
Plasmodium berghei PBANKA_0834300   gamma-tubulin complex component, putative
Plasmodium falciparum PF3D7_0933500   gamma-tubulin complex component, putative
Plasmodium knowlesi PKNH_0732200   gamma-tubulin complex component, putative
Plasmodium vivax PVX_087040   gamma-tubulin complex component, putative
Plasmodium yoelii PY01833   gamma-tubulin interacting protein
Saccharomyces cerevisiae YNL126W   Spc98p
Schistosoma japonicum Sjp_0005820   Gamma-tubulin complex component 3, putative
Schistosoma japonicum Sjp_0005810   Gamma-tubulin complex component 3 homolog, putative
Schistosoma mansoni Smp_159180   gamma-tubulin complex component 3 (gcp-3)
Schmidtea mediterranea mk4.003002.04  
Schmidtea mediterranea mk4.003002.03   Gamma-tubulin complex component 3
Trypanosoma brucei gambiense Tbg972.11.12670   gamma-tubulin complex subunit, putative,spindle pole body component alp6, putative
Trypanosoma brucei Tb927.11.11340   gamma-tubulin complex component 3
Trypanosoma brucei Tb11.v5.1036   gamma-tubulin complex subunit, putative
Trypanosoma congolense TcIL3000.11.12000   gamma-tubulin complex component 3
Trypanosoma cruzi TcCLB.511211.150   gamma-tubulin complex component 3
Toxoplasma gondii TGME49_310130   Spc97 / Spc98 family protein
Trichomonas vaginalis TVAG_117310   spindle pole body component alp6, putative
Trichomonas vaginalis TVAG_348130   gamma-tubulin complex component, putative
Trichomonas vaginalis TVAG_404950   spindle pole body component alp6, putative

Essentiality

PF3D7_0933500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.3150 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.3150 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.3150 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.3150 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YNL126W Saccharomyces cerevisiae inviable yeastgenome
TGME49_310130 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_0933500 (Plasmodium falciparum), gamma-tubulin complex component, putative
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