Detailed view for TcCLB.506401.220

Basic information

TDR Targets ID: 50115
Trypanosoma cruzi, electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.5357 | Length (AA): 564 | MW (Da): 62380 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05187   Electron transfer flavoprotein-ubiquinone oxidoreductase, 4Fe-4S
PF13450   NAD(P)-binding Rossmann-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004174   electron-transferring-flavoprotein dehydrogenase activity  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 550 1pn0 (A) 2 572 10.00 0 1 0.55 1
496 548 7fd1 (A) 3 55 23.00 0.0000055 0.16 0.31 -0.14
4 562 2gmh (A) 20 583 44.00 0 1 1.48613 -0.16
11 562 2gmh (A) 27 583 54.00 0 1 1.61912 -0.6
13 61 1y56 (A) 102 141 50.00 0.62 0.99 0.424879 0.33

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128337)

Species Accession Gene Product
Arabidopsis thaliana AT2G43400   electron-transfer flavoprotein--ubiquinone oxidoreductase
Brugia malayi Bm1_15260   hypothetical protein
Brugia malayi Bm1_57180   Probable electron transfer flavoprotein-ubiquinone oxidoreductase,mitochondrial precursor
Candida albicans CaO19.3175   similar to S. cerevisiae YOR356W
Candida albicans CaO19.10685   similar to S. cerevisiae YOR356W
Caenorhabditis elegans CELE_C05D11.12   Protein LET-721
Dictyostelium discoideum DDB_G0278849   electron transfer flavoprotein-ubiquinone oxidoreductase
Drosophila melanogaster Dmel_CG12140   Electron transfer flavoprotein-ubiquinone oxidoreductase
Escherichia coli b0043   putative oxidoreductase
Escherichia coli b1699   putative oxidoreductase
Homo sapiens ENSG00000171503   electron-transferring-flavoprotein dehydrogenase
Leishmania braziliensis LbrM.07.0660   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Leishmania donovani LdBPK_070660.1   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Leishmania infantum LinJ.07.0660   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Leishmania major LmjF.07.0600   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Leishmania mexicana LmxM.07.0600   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Loa Loa (eye worm) LOAG_08143   lethal protein 721
Mus musculus ENSMUSG00000027809   electron transferring flavoprotein, dehydrogenase
Neospora caninum NCLIV_040030   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Oryza sativa 4349116   Os10g0516300
Saccharomyces cerevisiae YOR356W   Cir2p
Schmidtea mediterranea mk4.001794.00  
Trypanosoma brucei gambiense Tbg972.8.780   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Trypanosoma brucei Tb927.8.1240   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Trypanosoma congolense TcIL3000_0_21750   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Trypanosoma congolense TcIL3000_8_880   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Trypanosoma cruzi TcCLB.508543.140   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Trypanosoma cruzi TcCLB.506401.220   electron transfer flavoprotein-ubiquinone oxidoreductase, putative
Toxoplasma gondii TGME49_265230   electron transfer flavoprotein-ubiquinone oxidoreductase

Essentiality

TcCLB.506401.220 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.1240 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1240 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1240 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.1240 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0043 Escherichia coli non-essential goodall
CELE_C05D11.12 Caenorhabditis elegans embryonic lethal wormbase
CELE_C05D11.12 Caenorhabditis elegans larval lethal wormbase
CELE_C05D11.12 Caenorhabditis elegans slow growth wormbase
TGME49_265230 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.506401.220 (Trypanosoma cruzi), electron transfer flavoprotein-ubiquinone oxidoreductase, putative
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