Detailed view for TcCLB.511229.110

Basic information

TDR Targets ID: 50225
Trypanosoma cruzi, glycyl-tRNA synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.8263 | Length (AA): 623 | MW (Da): 70096 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03129   Anticodon binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004820   glycine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0006426   glycyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 602 1ati (A) 2 504 34.00 0 1 0.79 1.01
506 622 1v95 (A) 6 126 13.00 0 0.77 0.32 -0.45
3 618 5e6m (A) 56 671 48.00 0 1 1.13216 0.68
12 617 4kqe (A) 65 670 45.00 0 1 1.49031 -0.25
504 599 1nyr (A) 541 637 16.00 0 0.57 0.415493 -1.15
538 608 1evl (A) 567 641 23.00 0 0.81 0.416365 -0.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127272)

Species Accession Gene Product
Arabidopsis thaliana AT1G29880   glycyl-tRNA synthetase / glycine--tRNA ligase
Babesia bovis BBOV_I003770   glycyl-tRNA synthetase, putative
Brugia malayi Bm1_23170   glycyl-tRNA synthetase
Candida albicans CaO19.8067   glycyl-tRNA synthase
Candida albicans CaO19.437   glycyl-tRNA synthase
Caenorhabditis elegans CELE_T10F2.1   Protein GARS-1, isoform B
Cryptosporidium hominis Chro.80123   glycyl-tRNA synthetase
Cryptosporidium parvum cgd8_1030   glycyl-tRNA synthetase
Dictyostelium discoideum DDB_G0284583   glycine-tRNA ligase
Drosophila melanogaster Dmel_CG6778   Glycyl-tRNA synthetase
Echinococcus granulosus EgrG_000976400   glycyl tRNA synthetase
Entamoeba histolytica EHI_073460   glycyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000976400   glycyl tRNA synthetase
Giardia lamblia GL50803_9011   Glycyl-tRNA synthetase
Homo sapiens ENSG00000106105   glycyl-tRNA synthetase
Leishmania braziliensis LbrM.35.4070   glycyl tRNA synthetase, putative
Leishmania donovani LdBPK_364030.1   glycyl tRNA synthetase, putative
Leishmania infantum LinJ.36.4030   glycyl tRNA synthetase, putative
Leishmania major LmjF.36.3840   glycyl tRNA synthetase, putative
Leishmania mexicana LmxM.36.3840   glycyl tRNA synthetase, putative
Loa Loa (eye worm) LOAG_05879   glycyl-tRNA synthetase
Mycobacterium leprae ML0826   PROBABLE GLYCYL-tRNA SYNTHETASE GLYS (GLYCINE--tRNA LIGASE) (GLYRS)
Mus musculus ENSMUSG00000029777   glycyl-tRNA synthetase
Mycobacterium tuberculosis Rv2357c   Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS)
Mycobacterium ulcerans MUL_3610   glycyl-tRNA synthetase
Neospora caninum NCLIV_029970   TRNA synthetase Gly, related
Oryza sativa 4346171   Os08g0538000
Plasmodium berghei PBANKA_1022800   glycine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1420400   glycine--tRNA ligase
Plasmodium knowlesi PKNH_1337600   glycine--tRNA ligase, putative
Plasmodium vivax PVX_085460   glycine--tRNA ligase, putative
Plasmodium yoelii PY01198   glycyl-tRNA synthetase
Saccharomyces cerevisiae YBR121C   glycine--tRNA ligase
Saccharomyces cerevisiae YPR081C   putative glycine--tRNA ligase
Schistosoma japonicum Sjp_0311960   ko:K01880 glycyl-tRNA synthetase, class II [EC6.1.1.14C], putative
Schistosoma japonicum Sjp_0039430   Glycyl-tRNA synthetase, putative
Schistosoma mansoni Smp_040800   glycyl-tRNA synthetase
Schmidtea mediterranea mk4.003880.05   GlycineALQ-tRNA ligase
Trypanosoma brucei gambiense Tbg972.11.10820   glycyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.11.9640   glycyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000.11.10140   glycyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.504017.79   glycyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.511229.110   glycyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_256990   glycyl-tRNA synthetase
Treponema pallidum TP0672   glycyl-tRNA synthetase
Theileria parva TP01_0460   glycyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_398690   glycyl-tRNA synthetase, putative

Essentiality

TcCLB.511229.110 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1400 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.1400 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.1400 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.1400 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T10F2.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T10F2.1 Caenorhabditis elegans larval arrest wormbase
CELE_T10F2.1 Caenorhabditis elegans slow growth wormbase
CELE_T10F2.1 Caenorhabditis elegans sterile wormbase
YBR121C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1022800 Plasmodium berghei Essential plasmo
TGME49_256990 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.511229.110 (Trypanosoma cruzi), glycyl-tRNA synthetase, putative
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