Detailed view for PF3D7_0930900

Basic information

TDR Targets ID: 5048
Plasmodium falciparum, NifU-like protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.4279 | Length (AA): 192 | MW (Da): 22624 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01106   NifU-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0051536   iron-sulfur cluster binding  
GO:0005506   iron ion binding  
GO:0016226   iron-sulfur cluster assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
105 183 1veh (A) 8 87 42.00 0 1 1.05 -1.32
109 183 1th5 (A) 154 226 22.00 0 0.95 0.68 -1.8
93 189 2m5o (A) 1 95 33.00 0 0.98 0.971608 -0.67
106 188 1xhj (A) 3 82 35.00 0 0.2 0.550292 0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Sporozoite, Male Gametocyte. Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Female Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, Oocyst, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 48 hs. Otto TD
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127523)

Species Accession Gene Product
Arabidopsis thaliana AT1G51390   NFU domain protein 5
Arabidopsis thaliana AT3G20970   NifU-like protein 4
Babesia bovis BBOV_III006970   NifU-like domain containing protein
Brugia malayi Bm1_49015   r10h10-like protein TO42
Candida albicans CaO19.6283   second version of conserved Nifu-like protein
Candida albicans CaO19.2067   similar to S. cerevisiae mitochondrial Nifu-like protein
Candida albicans CaO19.3485   potential third version of conserved Nifu-like protein
Candida albicans CaO19.13662   second version of conserved Nifu-like protein
Candida albicans CaO19.9614   similar to S. cerevisiae mitochondrial Nifu-like protein
Caenorhabditis elegans CELE_R10H10.1   Protein LPD-8
Dictyostelium discoideum DDB_G0285593   NIF system FeS cluster assembly domain-containing protein
Drosophila melanogaster Dmel_CG32500   CG32500 gene product from transcript CG32500-RA
Drosophila melanogaster Dmel_CG33502   CG33502 gene product from transcript CG33502-RA
Drosophila melanogaster Dmel_CG32857   CG32857 gene product from transcript CG32857-RA
Echinococcus granulosus EgrG_000603000   NFU1 iron sulfur cluster scaffold
Echinococcus multilocularis EmuJ_000603000   NFU1 iron sulfur cluster scaffold
Giardia lamblia GL50803_32838   hypothetical protein
Homo sapiens ENSG00000169599   NFU1 iron-sulfur cluster scaffold
Leishmania braziliensis LbrM.26.0190   hypothetical protein, conserved
Leishmania donovani LdBPK_260180.1   Scaffold protein Nfu/NifU N terminal/NifU-like domain containing protein, putative
Leishmania infantum LinJ.26.0180   hypothetical protein, conserved
Leishmania major LmjF.26.0190   hypothetical protein, conserved
Leishmania mexicana LmxM.26.0190   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02490   hypothetical protein
Mus musculus ENSMUSG00000029993   NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
Neospora caninum NCLIV_013030   Nitrogen-fixing NifU, C-terminal, related
Oryza sativa 4337851   Os05g0155300
Plasmodium berghei PBANKA_0831700   NifU-like protein, putative
Plasmodium falciparum PF3D7_0930900   NifU-like protein, putative
Plasmodium knowlesi PKNH_0729600   NifU-like protein, putative
Plasmodium vivax PVX_087160   NifU-like protein, putative
Plasmodium yoelii PY06884   hypothetical protein
Saccharomyces cerevisiae YKL040C   Nfu1p
Schistosoma japonicum Sjp_0065740   ko:K07400 thioredoxin-like protein, putative
Trypanosoma brucei gambiense Tbg972.7.1730   HIRA-interacting protein 5, putative
Trypanosoma brucei Tb927.7.1720   NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
Trypanosoma congolense TcIL3000_7_1250   HIRA-interacting protein 5, putative
Trypanosoma cruzi TcCLB.508207.130   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507519.90   hypothetical protein, conserved
Toxoplasma gondii TGME49_212930   NifU family domain-containing protein
Theileria parva TP04_0610   hypothetical protein
Trichomonas vaginalis TVAG_044500   conserved hypothetical protein
Trichomonas vaginalis TVAG_008840   conserved hypothetical protein
Trichomonas vaginalis TVAG_146780   conserved hypothetical protein
Trichomonas vaginalis TVAG_451860   conserved hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0413   NifU family protein

Essentiality

PF3D7_0930900 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.1720 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1720 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1720 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.1720 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R10H10.1 Caenorhabditis elegans embryonic arrest wormbase
CELE_R10H10.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_R10H10.1 Caenorhabditis elegans larval arrest wormbase
CELE_R10H10.1 Caenorhabditis elegans slow growth wormbase
PBANKA_0831700 Plasmodium berghei Dispensable plasmo
TGME49_212930 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0930900 (Plasmodium falciparum), NifU-like protein, putative
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