Detailed view for PF3D7_1203200

Basic information

TDR Targets ID: 5080
Plasmodium falciparum, signal recognition particle subunit SRP14
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 10.5209 | Length (AA): 104 | MW (Da): 11862 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02290   Signal recognition particle 14kD protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005786   signal recognition particle, endoplasmic reticulum targeting  
GO:0048500   signal recognition particle  
GO:0030942   endoplasmic reticulum signal peptide binding  
GO:0008312   7S RNA binding  
GO:0005515   protein binding  
GO:0045900   negative regulation of translational elongation  
GO:0006614   SRP-dependent cotranslational protein targeting to membrane  

Metabolic Pathways

Protein export (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 103 1e8o (B) 2 88 37.00 0 0.73 0.89 0.26
1 103 1914 (A) 2001 2088 37.00 0 0.68 1.04768 -0.22
4 87 5aox (B) 4 71 43.00 0.6 0.65 0.875992 0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, Oocyst, Ring, Female Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, sporozoite, early ring, early schizont, early trophozoite, late ring, Sporozoite, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 40 hs. Otto TD
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_129782)

Species Accession Gene Product
Arabidopsis thaliana AT2G43640   signal recognition particle subunit SRP14
Caenorhabditis elegans CELE_F25G6.8   Protein F25G6.8
Caenorhabditis elegans CELE_F25G6.9   Protein F25G6.9
Dictyostelium discoideum DDB_G0272590   signal recognition particle 14 kDa subunit
Dictyostelium discoideum DDB_G0273977   signal recognition particle 14 kDa subunit
Drosophila melanogaster Dmel_CG5417   Signal recognition particle protein 14
Echinococcus granulosus EgrG_000665200   signal recognition particle 14 kda protein
Entamoeba histolytica EHI_194075   signal recognition particle 14 domain containing protein
Echinococcus multilocularis EmuJ_000665200   signal recognition particle 14 kda protein
Homo sapiens ENSG00000140319   signal recognition particle 14kDa (homologous Alu RNA binding protein)
Loa Loa (eye worm) LOAG_11231   hypothetical protein
Mus musculus 102642637   signal recognition particle 14 kDa protein-like
Mus musculus ENSMUSG00000009549   signal recognition particle 14
Oryza sativa 4337050   Os04g0623700
Onchocerca volvulus OVOC12000   Signal recognition particle 14 kDa protein homolog
Plasmodium berghei PBANKA_0602200   signal recognition particle subunit SRP14, putative
Plasmodium falciparum PF3D7_1203200   signal recognition particle subunit SRP14
Plasmodium knowlesi PKNH_1303100   signal recognition particle subunit SRP14, putative
Plasmodium vivax PVX_084205   signal recognition particle subunit SRP14, putative
Plasmodium yoelii PY05285   hypothetical protein
Toxoplasma gondii TGME49_256050   signal recognition particle 14kd protein
Theileria parva TP03_0473   signal recognition particle, putative
Trichomonas vaginalis TVAG_260070   signal recognition particle 14 kD protein, putative

Essentiality

PF3D7_1203200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_F25G6.9 Caenorhabditis elegans embryonic lethal wormbase
TGME49_256050 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1203200 (Plasmodium falciparum), signal recognition particle subunit SRP14
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