Detailed view for TcCLB.510187.80

Basic information

TDR Targets ID: 51049
Trypanosoma cruzi, endonuclease V, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.5539 | Length (AA): 314 | MW (Da): 34879 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04493   Endonuclease V

Gene Ontology

Mouse over links to read term descriptions.
GO:0004519   endonuclease activity  
GO:0006281   DNA repair  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
58 314 3ga2 (A) 1 235 25.00 0 1 1.01577 0.08
59 308 5aoy (A) 10 247 36.00 0 1 1.22648 -0.25
94 312 4nsp (A) 45 251 42.00 0 1 1.15575 -0.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129213)

Species Accession Gene Product
Arabidopsis thaliana AT4G31150   endonuclease V family protein
Dictyostelium discoideum DDB_G0273907   endonuclease V
Dictyostelium discoideum DDB_G0275469   hypothetical protein
Dictyostelium discoideum DDB_G0273165   endonuclease V
Escherichia coli b3998   endonuclease V
Echinococcus granulosus EgrG_000465700   endonuclease v
Entamoeba histolytica EHI_033860   endonuclease V, putative
Entamoeba histolytica EHI_104930   endonuclease V, putative
Entamoeba histolytica EHI_071330   endonuclease V, putative
Entamoeba histolytica EHI_011230   endonuclease V, putative
Entamoeba histolytica EHI_142700   endonuclease V, putative
Entamoeba histolytica EHI_193830   endonuclease V, putative
Entamoeba histolytica EHI_101390   endonuclease V, putative
Echinococcus multilocularis EmuJ_000465700   endonuclease v
Homo sapiens ENSG00000173818   endonuclease V
Leishmania braziliensis LbrM.35.2560   endonuclease V, putative
Leishmania donovani LdBPK_362460.1   endonuclease V, putative
Leishmania infantum LinJ.36.2460   endonuclease V, putative
Leishmania major LmjF.36.2330   endonuclease V, putative
Leishmania mexicana LmxM.36.2330   endonuclease V, putative
Mus musculus ENSMUSG00000039850   endonuclease V
Oryza sativa 4341765   Os06g0663600
Schmidtea mediterranea mk4.000407.04  
Trypanosoma brucei gambiense Tbg972.10.8380   endonuclease V, putative
Trypanosoma brucei Tb927.10.6860   endonuclease v
Trypanosoma congolense TcIL3000_10_5890   endonuclease V, putative
Trypanosoma cruzi TcCLB.510187.80   endonuclease V, putative
Trichomonas vaginalis TVAG_312360   conserved hypothetical protein

Essentiality

TcCLB.510187.80 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.6860 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.6860 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.6860 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.6860 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3998 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.510187.80 (Trypanosoma cruzi), endonuclease V, putative
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