Detailed view for TcCLB.510685.10

Basic information

TDR Targets ID: 51457
Trypanosoma cruzi, calreticulin, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.4853 | Length (AA): 401 | MW (Da): 46235 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00262   Calreticulin family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005783   endoplasmic reticulum  
GO:0051082   unfolded protein binding  
GO:0005515   protein binding  
GO:0005509   calcium ion binding  
GO:0006457   protein folding  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
18 347 1jhn (A) 65 457 40.00 0 1 1.01 0.74
22 364 5hcf (A) 23 366 99.00 0 1 1.65006 -0.23
22 281 5hcf (A) 23 364 76.00 0 1 1.50748 -0.64
210 393 5cwh (A) 3 159 13.00 0 0.04 0.465953 -0.93
348 399 4o9b (A) 250 300 22.00 0 0.08 0.584776 -2.77
364 400 5hmo (A) 818 854 43.00 0.09 0.04 0.984969 -4.67

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5HCF:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129181)

Species Accession Gene Product
Arabidopsis thaliana AT1G09210   calreticulin 1b
Arabidopsis thaliana AT1G56340   calreticulin-1
Brugia malayi Bm1_23560   calreticulin precursor
Caenorhabditis elegans CELE_Y38A10A.5   Protein CRT-1
Dictyostelium discoideum DDB_G0283539   hypothetical protein
Drosophila melanogaster Dmel_CG9429   Calreticulin
Entamoeba histolytica EHI_136160   calreticulin, putative
Echinococcus multilocularis EmuJ_000653100   calreticulin
Echinococcus multilocularis EmuJ_000653200   calreticulin
Homo sapiens ENSG00000179218   calreticulin
Leishmania braziliensis LbrM.31.2940   calreticulin, putative
Leishmania donovani LdBPK_312670.1   calreticulin, putative
Leishmania infantum LinJ.31.2670   calreticulin, putative
Leishmania major LmjF.31.2600   calreticulin, putative
Leishmania mexicana LmxM.30.2600   calreticulin, putative
Loa Loa (eye worm) LOAG_07330   RAL-1 protein
Mus musculus ENSMUSG00000003814   calreticulin
Oryza sativa 4342826   Os07g0246200
Oryza sativa 4334675   Os03g0832200
Schistosoma japonicum Sjp_0056680   ko:K08057 calreticulin, putative
Schistosoma mansoni Smp_030370   calreticulin autoantigen homolog precursor
Schmidtea mediterranea mk4.000312.16   Calreticulin-2
Schmidtea mediterranea mk4.015079.00   Calreticulin
Schmidtea mediterranea mk4.002044.00   Calreticulin
Schmidtea mediterranea mk4.010882.00   Calreticulin
Schmidtea mediterranea mk4.000319.02   Calreticulin-1
Schmidtea mediterranea mk4.004837.02   Calreticulin
Schmidtea mediterranea mk4.031459.00   Calreticulin
Trypanosoma brucei gambiense Tbg972.4.5200   calreticulin, putative
Trypanosoma brucei Tb927.4.5010   calreticulin, putative
Trypanosoma brucei Tb927.8.7410   calreticulin, putative
Trypanosoma congolense TcIL3000_0_16790   calreticulin, putative
Trypanosoma congolense TcIL3000_4_4260   calreticulin, putative
Trypanosoma congolense TcIL3000_8_7620   calreticulin, putative
Trypanosoma cruzi TcCLB.510685.10   calreticulin, putative
Trypanosoma cruzi TcCLB.509011.40   calreticulin, putative
Trichomonas vaginalis TVAG_122020   calreticulin, putative
Trichomonas vaginalis TVAG_153450   calnexin, putative

Essentiality

TcCLB.510685.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7410 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7410 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7410 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.7410 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.4.5010 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.5010 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.5010 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.5010 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y38A10A.5 Caenorhabditis elegans larval lethal wormbase
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.510685.10 (Trypanosoma cruzi), calreticulin, putative
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