Detailed view for PF3D7_1211300

Basic information

TDR Targets ID: 5160
Plasmodium falciparum, DNA helicase MCM8, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.0089 | Length (AA): 1135 | MW (Da): 132607 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00493   MCM2/3/5 family
PF17207   MCM OB domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0006271   DNA strand elongation during DNA replication  
GO:0005656   pre-replicative complex  
GO:0005524   ATP binding  
GO:0004003   ATP-dependent DNA helicase activity  
GO:0003677   DNA binding  
GO:0006270   DNA replication initiation  
GO:0006260   DNA replication  

Metabolic Pathways

Purine metabolism (KEGG)

Structural information

Modbase 3D models:

There are 54 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
73 326 1ltl (A) 5 240 18.00 0 0.8 0.1 0.82
258 369 2ane (A) 10 117 14.00 0 0.01 0.23 -0.25
240 417 4pof (A) 90 254 22.00 0.0021 0.84 0.269028 0.73
320 661 1epw (A) 710 1133 30.00 0.061 0.06 0.0879216 1.59
334 498 4u5a (C) 47 239 21.00 0.12 0.06 0.283574 -0.01
645 1043 4r7z (A) 265 965 34.00 0.00094 1 0.450042 0.59
12 771 2yk0 (A) 19 715 30.00 0 1 0.715055 0.25
12 513 2yk0 (A) 19 509 34.00 0 1 0.614399 0.08
515 551 3bog (C) 3 79 99.99 0 0 1.03912 -0.26
677 1082 2c6j (A) 15 305 24.00 0.0000000000055 1 -0.0284736 0.67
896 1077 4uos (A) 0 182 15.00 0.82 0.12 0.372491 -1.35
1213 1360 4v3e (A) 563 710 28.00 0.00018 0.96 0.27897 -0.56
1255 1290 3c64 (A) 44 75 50.00 0.055 0.24 0.510452 -0.94
1379 1481 1w9r (A) 17 119 11.00 0.53 0 0.20393 -0.61
1403 1473 3v8u (A) 53 128 42.00 0.99 0.1 0.310739 -0.55
161 400 2vl6 (A) 20 262 20.00 0.002 0.53 0.255713 0.82
228 405 4pof (A) 90 254 22.00 0.0041 0.84 0.270504 0.73
308 649 1epw (A) 710 1133 30.00 0.061 0.06 0.0907414 1.59
334 498 4u5a (A) 48 224 20.00 0.88 0.08 0.288928 -0.03
369 1028 4r7y (A) 24 1962 27.00 0 1 0.649711 1.19
7 731 2yk0 (A) 19 715 31.00 0 1 0.754692 -0.35
7 732 2yk0 (A) 19 716 31.00 0 1 0.720091 -0.21
175 410 2y8d (A) 2425 2581 41.00 0.00000000013 0.91 0.031802 0.88
521 725 4v3d (A) 500 716 26.00 0.0000000059 1 0.565128 -1.37
835 1579 4p1t (A) 1224 1911 16.00 0 0.82 0.332675 0.91
835 1474 1zro (A) 8 576 19.00 0 0.98 0.0935632 0.82
839 1223 3cml (A) 1228 1529 35.00 0.0000000074 1 0.0900771 0.68
840 1267 2c6j (A) 15 298 22.00 0 1 -0.184459 0.65
1595 1637 2kpy (A) 55 105 47.00 0.57 0.01 0.463164 -1.59
15 815 2yk0 (A) 19 715 31.00 0 1 0.439735 0.54
16 814 2yk0 (A) 20 714 33.00 0 1 0.478353 0.44
270 306 3bog (C) 3 79 99.99 0 0 1.02406 -0.26
313 349 3bog (C) 3 79 99.99 0.024 0 1.03046 -0.26
601 816 4v3d (A) 500 716 36.00 0 1 0.527041 -0.72
665 817 3c64 (A) 2 166 40.00 0.00000014 0.91 0.325412 0.06
924 1664 4gf2 (A) 157 740 27.00 0 1 0.270679 0.79
1324 2068 4p1t (A) 1218 1855 23.00 0 0.99 0.183843 0.57
1334 1623 4nuu (A) 210 501 26.00 0 1 0.39237 -0.99
1349 1645 3vuu (A) 170 454 33.00 0 1 0.365706 -0.16
1844 2049 4uos (A) 1 184 11.00 0.049 0.3 0.0961317 -1.05
2063 2236 2ap3 (A) 9 194 13.00 0.31 0.05 0.193622 -0.7
2284 2458 4tql (A) 48 221 18.00 0.3 0.97 0.271413 -0.36
2319 2497 4tql (A) 48 217 27.00 0.041 1 0.348577 -0.45
2396 2511 1w9r (A) 6 119 23.00 0.015 0.33 0.265348 -0.57
2412 2499 1w9r (A) 15 105 36.00 0.11 0.2 0.404002 -0.56
19 778 2yk0 (A) 19 715 32.00 0 1 0.663458 -0.09
22 723 2yk0 (A) 22 671 37.00 0 1 0.701431 -0.17
187 432 2y8d (A) 2425 2594 38.00 0 0.65 0.0626366 0.65
539 733 4v3d (A) 500 693 37.00 0 1 0.437475 -0.43
540 754 4v3d (A) 500 716 30.00 0.00000000035 1 0.450519 -0.97
544 765 4v3d (C) 504 716 31.00 0.000027 1 0.355884 -0.51
621 777 3c64 (A) 2 163 45.00 0 1 0.445492 -0.54
1376 1559 4uos (A) 1 183 13.00 0.0053 0.11 0.299901 -0.95
1747 1791 1jvr (A) 91 134 30.00 0.79 0.11 0.285148 0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile sporozoite, Oocyst, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, late trophozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, early trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 8 hs. Otto TD
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_129017)

Species Accession Gene Product
Arabidopsis thaliana AT3G09660   minichromosome maintenance 8
Dictyostelium discoideum DDB_G0283009   MCM family protein
Drosophila melanogaster Dmel_CG31293   recombination-defective
Echinococcus granulosus EgrG_000772700   dna replication licensing factor mcm8
Entamoeba histolytica EHI_140670   DNA replication licensing factor, putative
Echinococcus multilocularis EmuJ_000772700   dna replication licensing factor mcm8
Homo sapiens ENSG00000125885   minichromosome maintenance complex component 8
Leishmania braziliensis LbrM.05.0320   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Leishmania donovani LdBPK_050330.1   DNA replication licensing factor, putative
Leishmania infantum LinJ.05.0330   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Leishmania major LmjF.05.0330   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Leishmania mexicana LmxM.05.0330   DNA replication licensing factor, putative,minichromosome maintenance protein-like protein
Mus musculus ENSMUSG00000027353   minichromosome maintenance deficient 8 (S. cerevisiae)
Neospora caninum NCLIV_058290   DNA replication licensing factor, putative
Oryza sativa 4339038   Os05g0464100
Plasmodium berghei PBANKA_0609800   DNA helicase MCM8, putative
Plasmodium falciparum PF3D7_1211300   DNA helicase MCM8, putative
Plasmodium knowlesi PKNH_1311400   DNA helicase MCM8, putative
Plasmodium vivax PVX_084595   DNA replication licensing factor MCM8, putative
Plasmodium yoelii PY02431   MCM2/3/5 family
Schistosoma japonicum Sjp_0046030   Conserved hypothetical protein
Schistosoma japonicum Sjp_0046020   expressed protein
Schistosoma japonicum Sjp_0098620   DNA replication licensing factor MCM8, putative
Schistosoma japonicum Sjp_0119450   hypothetical protein
Schistosoma japonicum Sjp_0079950   ko:K10737 minichromosome maintenance protein 8, putative
Schistosoma japonicum Sjp_0099600   hypothetical protein
Schistosoma mansoni Smp_169450   DNA replication licensing factor MCM8
Schmidtea mediterranea mk4.000367.10   DNA replication licensing factor MCM8, putative
Schmidtea mediterranea mk4.000367.11   DNA replication licensing factor REC
Trypanosoma brucei gambiense Tbg972.10.12700   minichromosome maintenance (MCM) complex subunit, putative,minichromosome maintenance protein-like protein
Trypanosoma brucei Tb927.10.10410   DNA replication licensing factor MCM8, putative
Trypanosoma brucei Tb11.v5.0535   minichromosome maintenance (MCM) complex subunit, putative
Trypanosoma congolense TcIL3000_10_8890   DNA replication licensing factor MCM8, putative
Trypanosoma cruzi TcCLB.503425.29   DNA replication licensing factor MCM8, putative
Trypanosoma cruzi TcCLB.503555.10   DNA replication licensing factor MCM8, putative
Toxoplasma gondii TGME49_315600   MCM2/3/5 family protein
Theileria parva TP01_0666   DNA replication licensing factor MCM4, putative

Essentiality

PF3D7_1211300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.10410 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.10410 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.10410 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.10410 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_315600 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1211300 (Plasmodium falciparum), DNA helicase MCM8, putative
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