Detailed view for TcCLB.503479.70

Basic information

TDR Targets ID: 51699
Trypanosoma cruzi, hook complex protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1422 | Length (AA): 1271 | MW (Da): 143534 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 18 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 371 1nex (B) 274 744 14.00 0 1 -0.12 0.56
8 321 1p22 (A) 217 545 20.00 0.000000000028 1 0.39 -0.06
14 325 1erj (A) 285 710 14.00 0 1 -0.07 0.27
73 372 1erj (A) 337 709 18.00 0 1 0.44 -0.53
585 1031 1hci (A) 276 720 16.00 0.000019 0.8 0.34 1.05
2 322 3ei3 (B) 130 454 14.00 0 0.82 0.216857 0.25
7 321 5cxb (A) 122 469 27.00 0.0025 0.95 0.152236 1.43
30 321 2ymu (A) 283 577 26.00 0.000000000095 1 0.44414 0.18
95 291 4j87 (A) 26 295 35.00 0.0000001 0.52 0.00939607 1.3
121 321 3frx (A) 16 224 32.00 0.0000019 0.68 0.329543 0.39
125 332 1erj (A) 446 669 23.00 0.018 0.76 0.357051 -0.05
161 322 4cy3 (A) 69 227 30.00 0.00000047 0.52 0.368859 -0.17
241 276 4u7a (A) 429 464 44.00 0.83 0.3 0.450724 0.4
246 318 5cxb (B) 450 513 38.00 0.27 0.77 0.380835 -0.14
413 617 5cwp (A) 5 202 14.00 0 0.31 0.34759 -1.43
454 614 3l4q (C) 431 576 14.00 0 0.49 0.225972 -1.03
472 962 1hci (A) 278 734 14.00 0 1 0.39461 0.51
497 581 5j0k (A) 5 74 40.00 0.91 0.11 0.283276 -0.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_154337)

Species Accession Gene Product
Schmidtea mediterranea mk4.000772.03  
Trypanosoma brucei gambiense Tbg972.10.920  
Trypanosoma brucei gambiense Tbg972.10.930  
Trypanosoma brucei Tb927.10.850   hook complex protein, conserved
Trypanosoma cruzi TcCLB.503479.70   hook complex protein, conserved

Essentiality

TcCLB.503479.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.850 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.850 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.850 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.850 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.503479.70 (Trypanosoma cruzi), hook complex protein, conserved
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