Detailed view for PKNH_1251600

Basic information

TDR Targets ID: 528446
Plasmodium knowlesi, tyrosine kinase-like protein, putative
Source Database / ID: 

pI: 6.7688 | Length (AA): 1633 | MW (Da): 186912 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07647   SAM domain (Sterile alpha motif)
PF07714   Protein tyrosine kinase
PF12330   Haspin like kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
900 1056 4v3d (A) 500 650 17.00 0 0.93 0.249342 -0.9
1021 1061 5hmo (A) 814 852 41.00 0.43 0.05 0.911107 -5.38
1275 1602 5dyk (A) 466 781 23.00 0 1 0.371257 0.52
1389 1615 3zep (B) 862 1098 30.00 0.000000000032 1 0.404008 -0.42
1408 1602 3aqv (A) 75 256 39.00 0 1 0.451412 0.21
1408 1615 4k33 (A) 537 751 29.00 0.00000000024 1 0.469373 -0.39

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_142857)

Species Accession Gene Product
Cryptosporidium hominis Chro.30486   sexual stage-specific protein kinase
Cryptosporidium parvum cgd3_4310   sexual stage-specific protein kinase
Neospora caninum NCLIV_008190   hypothetical protein
Plasmodium berghei PBANKA_1362100   tyrosine kinase-like protein, putative
Plasmodium falciparum PF3D7_1349300   tyrosine kinase-like protein
Plasmodium knowlesi PKNH_1251600   tyrosine kinase-like protein, putative
Plasmodium vivax PVX_083360   tyrosine kinase-like protein, putative
Plasmodium yoelii PY04013   sexual stage-specific protein kinase
Toxoplasma gondii TGME49_253860   Tyrosine kinase-like (TKL) protein

Essentiality

PKNH_1251600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1362100 Plasmodium berghei Slow plasmo
TGME49_253860 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Sus scrofa Casein kinase I isoform alpha 125 aa 25.0% 120 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 27.0% 215 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 27.7% 173 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PKNH_1251600 (Plasmodium knowlesi), tyrosine kinase-like protein, putative
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