Detailed view for TcCLB.506933.40

Basic information

TDR Targets ID: 52856
Trypanosoma cruzi, DNA replication licensing factor MCM2, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.1479 | Length (AA): 953 | MW (Da): 108986 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00493   MCM2/3/5 family
PF14551   MCM N-terminal domain
PF17207   MCM OB domain

Gene Ontology

Mouse over links to read term descriptions.
GO:1905775   GO:negative regulation of DNA helicase activity  

GO:0042555   MCM complex  
GO:0005634   nucleus  
GO:0005524   ATP binding  
GO:0003677   DNA binding  
GO:0006270   DNA replication initiation  
GO:0006260   DNA replication  

Metabolic Pathways

DNA replication (KEGG)

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
198 445 1ltl (A) 7 240 24.00 0 1 0.46 -0.68
483 861 1g8p (A) 18 349 19.00 0 1 -0.04 0.87
4 140 5h78 (A) 58 192 7.00 0 0 0.278757 -1.03
33 90 5bnx (C) 69 120 33.00 0 0.01 0.13786 1.11
197 449 4pof (A) 3 249 30.00 0 1 0.620577 -0.12
200 847 4fdg (B) 10 598 36.00 0 1 0.873958 1.13
462 847 4r7z (A) 263 962 40.00 0 1 0.586037 0.36
696 898 5cwp (A) 21 229 25.00 0.93 0.96 0.392112 -0.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127764)

Species Accession Gene Product
Arabidopsis thaliana AT1G44900   minichromosome maintenance protein 2
Babesia bovis BBOV_II005900   DNA replication licensing factor MCM2, putative
Brugia malayi Bm1_47040   DNA replication licensing factor MCM2
Candida albicans CaO19.4354   ATP-dependent DNA helicase
Candida albicans CaO19.11832   ATP-dependent DNA helicase
Caenorhabditis elegans CELE_Y17G7B.5   Protein MCM-2, isoform B
Cryptosporidium hominis Chro.20122   DNA replication licensing factor MCM2
Cryptosporidium parvum cgd2_1100   DNA replication licensing factor MCM2 like AAA+ ATpase
Dictyostelium discoideum DDB_G0286623   MCM family protein
Drosophila melanogaster Dmel_CG7538   Minichromosome maintenance 2
Echinococcus granulosus EgrG_000212600   DNA replication licensing factor MCM2
Entamoeba histolytica EHI_117970   DNA replication licensing factor
Echinococcus multilocularis EmuJ_000212600   DNA replication licensing factor MCM2
Giardia lamblia GL50803_15344   MCM2
Homo sapiens ENSG00000073111   minichromosome maintenance complex component 2
Leishmania braziliensis LbrM.28.0920   minichromosome maintenance (MCM) complex subunit, putative
Leishmania donovani LdBPK_280940.1   minichromosome maintenance (MCM) complex subunit, putative
Leishmania infantum LinJ.28.0940   minichromosome maintenance (MCM) complex subunit, putative
Leishmania major LmjF.28.0850   minichromosome maintenance (MCM) complex subunit, putative
Leishmania mexicana LmxM.28.0850   minichromosome maintenance (MCM) complex subunit, putative
Loa Loa (eye worm) LOAG_09778   DNA replication licensing factor MCM2
Mus musculus ENSMUSG00000002870   minichromosome maintenance deficient 2 mitotin (S. cerevisiae)
Neospora caninum NCLIV_052000   DNA replication licensing factor, putative
Oryza sativa 4350526   Os11g0484300
Plasmodium berghei PBANKA_1024900   DNA replication licensing factor MCM2, putative
Plasmodium falciparum PF3D7_1417800   DNA replication licensing factor MCM2
Plasmodium knowlesi PKNH_1340200   DNA replication licensing factor MCM2, putative
Plasmodium vivax PVX_085565   DNA replication licensing factor MCM2, putative
Plasmodium yoelii PY01644   DNA replication licensing factor MCM2
Saccharomyces cerevisiae YBL023C   MCM DNA helicase complex subunit MCM2
Schistosoma japonicum Sjp_0072100   DNA replication licensing factor MCM2, putative
Schistosoma japonicum Sjp_0072090   ko:K02540 minichromosome maintenance protein 2, putative
Schistosoma japonicum Sjp_0072080   ko:K02540 minichromosome maintenance protein 2, putative
Schistosoma mansoni Smp_079560   DNA replication licensing factor MCM2
Schistosoma mansoni Smp_054840   DNA replication licensing factor MCM2
Schmidtea mediterranea mk4.003649.01   DNA replication licensing factor MCM2
Schmidtea mediterranea mk4.005218.00   DNA replication licensing factor MCM2
Trypanosoma brucei gambiense Tbg972.11.8960   minichromosome maintenance (MCM) complex subunit, putative
Trypanosoma brucei Tb927.11.7860   DNA replication licensing factor MCM2
Trypanosoma congolense TcIL3000.11.8400   DNA replication licensing factor MCM2, putative
Trypanosoma cruzi TcCLB.506933.40   DNA replication licensing factor MCM2, putative
Toxoplasma gondii TGME49_214970   DNA replication licensing factor, putative
Theileria parva TP02_0532   DNA replication licensing factor MCM2, putative
Trichomonas vaginalis TVAG_122470   DNA replication licensing factor MCM4, putative

Essentiality

TcCLB.506933.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.5730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5730 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.5730 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5730 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y17G7B.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y17G7B.5 Caenorhabditis elegans larval arrest wormbase
CELE_Y17G7B.5 Caenorhabditis elegans slow growth wormbase
CELE_Y17G7B.5 Caenorhabditis elegans sterile wormbase
YBL023C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1024900 Plasmodium berghei Essential plasmo
TGME49_214970 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Urotensin-2 123 aa 28.7% 101 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.506933.40 (Trypanosoma cruzi), DNA replication licensing factor MCM2, putative
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