Detailed view for PKNH_1117000

Basic information

TDR Targets ID: 529178
Plasmodium knowlesi, metacaspase 1, putative
Source Database / ID: 

pI: 8.0786 | Length (AA): 642 | MW (Da): 73478 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00656   Caspase domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
136 237 4gcn (A) 7 106 4.00 0.0044 0 0.281278 -1.2
300 604 4afv (A) 29 334 31.00 0 1 0.797278 -0.53
328 491 2h4y (A) 141 289 16.00 0 0.41 0.255652 -0.1
346 604 4af8 (A) 74 334 36.00 0 1 0.743427 -0.45
533 630 2a8b (A) 409 500 34.00 0.28 0.06 0.319648 0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129045)

Species Accession Gene Product
Arabidopsis thaliana AT1G02170   metacaspase 1
Arabidopsis thaliana AT4G25110   metacaspase 2
Candida albicans CaO19.5995   similar to S. cerevisiae MCA1 (YOR197W) putative cysteine protease involved in apoptosis
Candida albicans CaO19.13416   similar to S. cerevisiae MCA1 (YOR197W) putative cysteine protease involved in apoptosis
Leishmania braziliensis LbrM.34.1490   metacaspase, putative
Leishmania donovani LdBPK_351580.1   metacaspase, putative
Leishmania infantum LinJ.35.1580   metacaspase, putative
Leishmania major LmjF.35.1580   metacaspase, putative
Leishmania mexicana LmxM.34.1580   metacaspase, putative
Neospora caninum NCLIV_044210   hypothetical protein
Oryza sativa 4349416   Os10g0565100
Plasmodium berghei PBANKA_1131400   metacaspase 1
Plasmodium falciparum PF3D7_1354800   metacaspase 1
Plasmodium knowlesi PKNH_1117000   metacaspase 1, putative
Plasmodium vivax PVX_114725   metacaspase 1, putative
Plasmodium yoelii PY04890   hypothetical protein
Saccharomyces cerevisiae YOR197W   Ca(2+)-dependent cysteine protease MCA1
Trypanosoma brucei gambiense Tbg972.9.8930   metacaspase 5, putative
Trypanosoma brucei gambiense Tbg972.6.700   metacaspase MCA3
Trypanosoma brucei gambiense Tbg972.6.710   metacaspase MCA2, putative,cysteine peptidase, Clan CD, family C13, putative
Trypanosoma brucei Tb927.6.930   metacaspase MCA3
Trypanosoma brucei Tb927.6.940   metacaspase MCA2
Trypanosoma brucei Tb927.9.14220   metacaspase 5, putative
Trypanosoma congolense TcIL3000_0_46570   metacaspase MCA2
Trypanosoma congolense TcIL3000_9_6120   metacaspase 5, putative
Trypanosoma cruzi TcCLB.506999.40   metacaspase 5, putative
Trypanosoma cruzi TcCLB.510759.160   metacaspase 5, putative
Trypanosoma cruzi TcCLB.507297.30   metacaspase, putative
Trypanosoma cruzi TcCLB.509399.20   metacaspase, putative
Trypanosoma cruzi TcCLB.507537.40   metacaspase, putative
Trypanosoma cruzi TcCLB.506531.50   metacaspase, putative
Toxoplasma gondii TGME49_206490   ICE family protease (caspase) p20 domain-containing protein

Essentiality

PKNH_1117000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.930 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.930 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.930 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.930 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.211.4760 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.4760 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.4760 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.4760 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1131400 Plasmodium berghei Dispensable plasmo
TGME49_206490 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma brucei gambiense metacaspase MCA2, putative,cysteine peptidase, Clan CD, family C13, putative Compounds References
Trypanosoma brucei metacaspase MCA2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PKNH_1117000 (Plasmodium knowlesi), metacaspase 1, putative
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