Detailed view for TcCLB.506963.40

Basic information

TDR Targets ID: 53429
Trypanosoma cruzi, Protein of unknown function (DUF498/DUF598), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3716 | Length (AA): 627 | MW (Da): 72479 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04430   Protein of unknown function (DUF498/DUF598)

Gene Ontology

Mouse over links to read term descriptions.
GO:0032981   mitochondrial respiratory chain complex I assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
247 370 2gm2 (A) 1 123 26.00 0 1 0.53 -1.42
253 367 2fi9 (A) 11 124 21.00 0 0.99 0.64 -1.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_129955)

Species Accession Gene Product
Arabidopsis thaliana AT2G44525   hypothetical protein
Arabidopsis thaliana AT3G60150   hypothetical protein
Brugia malayi Bm1_19465   hypothetical protein
Caenorhabditis elegans CELE_R07H5.3   Protein NUAF-3, isoform C
Dictyostelium discoideum DDB_G0292722   hypothetical protein
Drosophila melanogaster Dmel_CG5569   CG5569 gene product from transcript CG5569-RA
Echinococcus granulosus EgrG_000315300   expressed protein
Echinococcus multilocularis EmuJ_000315300   expressed protein
Homo sapiens 25915   NADH dehydrogenase (ubiquinone) complex I, assembly factor 3
Leishmania braziliensis LbrM.35.3960   hypothetical protein, conserved
Leishmania donovani LdBPK_363910.1   Protein of unknown function (DUF498/DUF598), putative
Leishmania infantum LinJ.36.3910   hypothetical protein, conserved
Leishmania major LmjF.36.3720   hypothetical protein, conserved
Leishmania mexicana LmxM.36.3720   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06313   hypothetical protein
Mus musculus 66706   NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
Oryza sativa 9268800   Os03g0590700
Oryza sativa 4349453   Os10g0571100
Schistosoma mansoni Smp_001590.1   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.10940   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.9750   Protein of unknown function (DUF498/DUF598), putative
Trypanosoma congolense TcIL3000.11.10230   Protein of unknown function (DUF498/DUF598), putative
Trypanosoma cruzi TcCLB.511233.40   Protein of unknown function (DUF498/DUF598), putative
Trypanosoma cruzi TcCLB.506963.40   Protein of unknown function (DUF498/DUF598), putative
Wolbachia endosymbiont of Brugia malayi Wbm0174   hypothetical protein

Essentiality

TcCLB.506963.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.1500 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.1500 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) complex I, assembly factor 3 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.506963.40 (Trypanosoma cruzi), Protein of unknown function (DUF498/DUF598), putative
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