Detailed view for PF3D7_1231500

Basic information

TDR Targets ID: 5356
Plasmodium falciparum, mitosis protein dim1, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.9724 | Length (AA): 139 | MW (Da): 16539 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02966   Mitosis protein DIM1

Gene Ontology

Mouse over links to read term descriptions.
GO:0046540   U4/U6 x U5 tri-snRNP complex  
GO:0000398   nuclear mRNA splicing, via spliceosome  
GO:0005681   spliceosome  
GO:0007067   mitosis  
GO:0000245   spliceosome assembly  

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 133 2av4 (A) 22 154 93.00 0 1 2.29 -3.16
1 135 1qgv (A) 4 138 77.00 0 1 2.05 -2.56
1 133 2av4 (A) 22 154 93.00 0 1 2.21893 -2.39
1 137 4cdo (A) 4 246 76.00 0 1 2.00371 -1.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2AV4:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, merozoite, sporozoite, early ring, late ring, late schizont, Ring, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, early trophozoite, late trophozoite, Oocyst, Sporozoite, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_128171)

Species Accession Gene Product
Arabidopsis thaliana AT5G08290   thioredoxin-like protein YLS8
Babesia bovis BBOV_IV011170   dim1 protein-like protein, putative
Brugia malayi Bm1_33505   Chain A, Human Spliceosomal Protein U5-15kd.
Candida albicans CaO19.1975   spliceosomal U5 snRNP 15kd protein
Candida albicans CaO19.9531   spliceosomal U5 snRNP 15kd protein
Caenorhabditis elegans CELE_Y54G2A.75   Protein Y54G2A.75
Cryptosporidium parvum cgd8_3690   mitosis protein DIM1
Dictyostelium discoideum DDB_G0275407   DIM1-like protein
Drosophila melanogaster Dmel_CG3058   CG3058 gene product from transcript CG3058-RA
Echinococcus granulosus EgrG_001045900   mitosis protein dim1
Entamoeba histolytica EHI_045260   DIM1 family protein, putative
Echinococcus multilocularis EmuJ_001045900   mitosis protein dim1
Giardia lamblia GL50803_15500   Hypothetical protein
Homo sapiens 10907   thioredoxin-like 4A
Leishmania braziliensis LbrM.23.0750   spliceosomal U5 snRNP-specific protein, putative,DIM-like protein, putative
Leishmania donovani LdBPK_230820.1   spliceosomal U5 snRNP-specific protein, putative
Leishmania infantum LinJ.23.0820   spliceosomal U5 snRNP-specific protein, putative,DIM-like protein, putative
Leishmania major LmjF.23.0650   spliceosomal U5 snRNP-specific protein, putative,DIM-like protein, putative
Leishmania mexicana LmxM.23.0650   spliceosomal U5 snRNP-specific protein, putative,DIM-like protein, putative
Loa Loa (eye worm) LOAG_02998   thioredoxin-like protein 4A
Mus musculus 27366   thioredoxin-like 4A
Neospora caninum NCLIV_036500   hypothetical protein
Oryza sativa 4348949   Os10g0486600
Oryza sativa 9268087   Os07g0202450
Plasmodium berghei PBANKA_1446200   mitosis protein dim1, putative
Plasmodium falciparum PF3D7_1231500   mitosis protein dim1, putative
Plasmodium knowlesi PKNH_1450900   mitosis protein dim1, putative
Plasmodium vivax PVX_100520   mitosis protein dim1, putative
Plasmodium yoelii PY07357   Drosophila melanogaster RE13747p
Saccharomyces cerevisiae YPR082C   Dib1p
Schistosoma japonicum Sjp_0307100   Thioredoxin-like protein 4A, putative
Schistosoma mansoni Smp_106050   mitosis protein dim1
Schmidtea mediterranea mk4.003751.00   Thioredoxin-like protein 4A
Schmidtea mediterranea mk4.003306.07   Thioredoxin-like protein 4A
Schmidtea mediterranea mk4.007622.04   Thioredoxin-like protein 4A
Trypanosoma brucei gambiense Tbg972.8.2050   spliceosomal U5 snRNP-specific protein, putative
Trypanosoma brucei Tb927.8.2560   spliceosomal U5 snRNP-specific protein, putative
Trypanosoma cruzi TcCLB.503829.89   spliceosomal U5 snRNP-specific protein, putative
Trypanosoma cruzi TcCLB.508045.79   spliceosomal U5 snRNP-specific protein, putative
Toxoplasma gondii TGME49_270140   splicing factor DIM1, putative
Theileria parva TP01_0642   mitosis protein dim1, putative
Trichomonas vaginalis TVAG_307980   mitosis protein dim1, putative

Essentiality

PF3D7_1231500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2560 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.2560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y54G2A.75 Caenorhabditis elegans embryonic lethal wormbase
YPR082C Saccharomyces cerevisiae inviable yeastgenome
TGME49_270140 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1231500 (Plasmodium falciparum), mitosis protein dim1, putative
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