Detailed view for TcCLB.503677.4

Basic information

TDR Targets ID: 54158
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.2589 | Length (AA): 92 | MW (Da): 10629 | Paralog Number: 4

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01412   Putative GTPase activating protein for Arf

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0008060   ARF GTPase activator activity  
GO:0005096   GTPase activator activity  
GO:0032312   regulation of ARF GTPase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 92 2crr (A) 3 93 49.00 0 1 1.5 -0.02
13 91 2b0o (E) 423 501 39.00 0 1 1.34 -0.79
2 92 2crr (A) 3 93 49.00 0 1 1.58393 -0.25
8 91 3lju (X) 0 80 48.00 0 0.99 1.56684 -1.07
8 92 2owa (A) 19 100 41.00 0 0.95 1.41371 -0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127190)

Species Accession Gene Product
Arabidopsis thaliana AT3G17660   putative ADP-ribosylation factor GTPase-activating protein AGD15
Arabidopsis thaliana AT5G54310   putative ADP-ribosylation factor GTPase-activating protein AGD5
Babesia bovis BBOV_IV001120   GTP-ase activating domain containing protein
Brugia malayi Bm1_38220   GTP-ase activating protein for Arf containing protein
Candida albicans CaO19.1396   potential ADP ribosylation factor GTPase activating protein (ARF GAP) similar to S. cerevisiae AGE2 (YIL044C) ARF GAP with a rol
Candida albicans CaO19.8974   potential ADP ribosylation factor GTPase activating protein (ARF GAP) similar to S. cerevisiae AGE2 (YIL044C) ARF GAP with a rol
Caenorhabditis elegans CELE_W09D10.1   Protein W09D10.1
Cryptosporidium hominis Chro.20191   homeobox-containing protein
Cryptosporidium parvum cgd2_1760   gata/ArfGAP, putative
Dictyostelium discoideum DDB_G0275603   Arf GTPase activating protein
Drosophila melanogaster Dmel_CG8243   CG8243 gene product from transcript CG8243-RA
Echinococcus granulosus EgrG_000469800   GTP ase activating protein for Arf containing
Entamoeba histolytica EHI_140140   gtpase activating protein, putative
Echinococcus multilocularis EmuJ_000469800   GTP ase activating protein for Arf containing
Giardia lamblia GL50803_17561   ARF GAP
Homo sapiens ENSG00000084070   small ArfGAP2
Homo sapiens ENSG00000112305   small ArfGAP 1
Leishmania braziliensis LbrM.29.0270   hypothetical protein, conserved
Leishmania braziliensis LbrM.32.1740   hypothetical protein, conserved
Leishmania donovani LdBPK_321650.1   Putative GTPase activating protein for Arf, putative
Leishmania donovani LdBPK_290340.1   Putative GTPase activating protein for Arf, putative
Leishmania infantum LinJ.32.1650   hypothetical protein, conserved
Leishmania infantum LinJ.29.0340   hypothetical protein, conserved
Leishmania major LmjF.32.1570   hypothetical protein, conserved
Leishmania major LmjF.29.0330   hypothetical protein, conserved
Leishmania mexicana LmxM.08_29.0330   hypothetical protein, conserved
Leishmania mexicana LmxM.31.1570   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06011   GTP-ase activating protein for Arf containing protein
Mus musculus ENSMUSG00000026155   small ArfGAP 1
Mus musculus ENSMUSG00000032870   small ArfGAP 2
Neospora caninum NCLIV_053650   hypothetical protein
Oryza sativa 4343612   Os07g0563800
Onchocerca volvulus OVOC4242   Putative smap1
Plasmodium berghei PBANKA_1241000   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium falciparum PF3D7_0526200   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium knowlesi PKNH_1006600   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium vivax PVX_079935   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium yoelii PY03864   homeobox-containing protein
Saccharomyces cerevisiae YIL044C   Age2p
Schistosoma japonicum Sjp_0003170   Stromal membrane-associated protein 2, putative
Schistosoma mansoni Smp_142850   smap1
Schmidtea mediterranea mk4.000554.03   Putative smap1
Schmidtea mediterranea mk4.000575.03  
Trypanosoma brucei gambiense Tbg972.11.10260   ADP-ribosylation factor GTPase activating protein, putative,centaurin beta 5
Trypanosoma brucei gambiense Tbg972.3.6030   ADP-ribosylation factor GTPase activating protein, putative,centaurin beta 5
Trypanosoma brucei Tb927.3.5330   centaurin beta 5
Trypanosoma brucei Tb927.11.9180   ADP-ribosylation factor GTPase activating protein, putative
Trypanosoma congolense TcIL3000.11.9540   ADP-ribosylation factor GTPase activating protein, putative
Trypanosoma congolense TcIL3000_3_3280   centaurin beta 5
Trypanosoma cruzi TcCLB.511717.100   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503677.4   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510901.110   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510143.30   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508409.220   hypothetical protein, conserved
Toxoplasma gondii TGME49_309220   GTPase activating protein for Arf protein
Theileria parva TP01_0136   ADP-ribosylation factor GTPase-activating protein, putative
Trichomonas vaginalis TVAG_462760   centaurin beta, putative
Trichomonas vaginalis TVAG_221710   centaurin gamma, putative
Trichomonas vaginalis TVAG_222000   smap1, putative
Trichomonas vaginalis TVAG_348220   arf-GTPase activating protein, putative
Trichomonas vaginalis TVAG_479200   HIV-1 rev binding protein, hrbl, putative
Trichomonas vaginalis TVAG_319720   arf-GTPase activating protein, putative
Trichomonas vaginalis TVAG_416020   centaurin delta, putative
Trichomonas vaginalis TVAG_198080   centaurin delta, putative
Trichomonas vaginalis TVAG_010830   centaurin/arf, putative

Essentiality

TcCLB.503677.4 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.0920 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.0920 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.0920 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.0920 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.3.5330 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.5330 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.5330 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.3.5330 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_W09D10.1 Caenorhabditis elegans larval arrest wormbase
CELE_W09D10.1 Caenorhabditis elegans larval lethal wormbase
CELE_W09D10.1 Caenorhabditis elegans slow growth wormbase
PBANKA_1241000 Plasmodium berghei Dispensable plasmo
TGME49_309220 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.503677.4 (Trypanosoma cruzi), hypothetical protein, conserved
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