Detailed view for Tbg972.4.5240

Basic information

TDR Targets ID: 543379
Trypanosoma brucei gambiense, dihydrolipoamide dehydrogenase, putative,acetoin dehydrogenase e3 component, putative
Source Database / ID: 

pI: 7.613 | Length (AA): 521 | MW (Da): 56835 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02852   Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain
PF07992   Pyridine nucleotide-disulphide oxidoreductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0009055   electron carrier activity  
GO:0050660   FAD binding  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 481 2a8x (A) 3 459 31.00 0 1 1.28732 -0.21
1 495 4j56 (A) 41 540 13.00 0 1 0.984396 0.7
1 38 1vg9 (A) 8 46 34.00 0 0.29 0.322237 0.67
3 41 1y56 (A) 108 145 50.00 0.39 0.72 0.529956 0.9
492 521 2jtq (A) 2 31 7.00 0.54 0 0.147382 -0.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_138783)

Species Accession Gene Product
Leishmania braziliensis LbrM.31.2990   acetoin dehydrogenase e3 component-like protein
Leishmania donovani LdBPK_312720.1   acetoin dehydrogenase e3 component-like protein
Leishmania infantum LinJ.31.2720   acetoin dehydrogenase e3 component-like protein
Leishmania major LmjF.31.2650   acetoin dehydrogenase e3 component-like protein
Leishmania mexicana LmxM.30.2650   acetoin dehydrogenase e3 component-like protein
Trypanosoma brucei gambiense Tbg972.4.5240   dihydrolipoamide dehydrogenase, putative,acetoin dehydrogenase e3 component, putative
Trypanosoma brucei gambiense Tbg972.8.7640   dihydrolipoamide dehydrogenase, point mutation,acetoin dehydrogenase e3 component, putative
Trypanosoma brucei Tb927.8.7380   dihydrolipoamide dehydrogenase, point mutation
Trypanosoma congolense TcIL3000_0_03450   dihydrolipoamide dehydrogenase, point mutation
Trypanosoma congolense TcIL3000_4_4310   dihydrolipoamide dehydrogenase, point mutation
Trypanosoma congolense TcIL3000_8_7580   dihydrolipoamide dehydrogenase, point mutation, putative
Trypanosoma cruzi TcCLB.507757.70   dihydrolipoamide dehydrogenase, putative
Trypanosoma cruzi TcCLB.508933.20   dihydrolipoamide dehydrogenase, putative

Essentiality

Tbg972.4.5240 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7380 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.7380 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7380 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.7380 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative Compounds References
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major trypanothione reductase 491 aa 20.1% 498 aa Compounds References
Rattus norvegicus Thioredoxin reductase 1, cytoplasmic 499 aa 23.2% 491 aa Compounds References
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative 510 aa 42.9% 511 aa Compounds References
Plasmodium falciparum glutathione reductase 500 aa 21.0% 529 aa Compounds References
Crithidia fasciculata Trypanothione reductase 491 aa 21.3% 498 aa Compounds References
Rattus norvegicus Glutathione reductase 424 aa 24.6% 382 aa Compounds References
Trypanosoma cruzi dihydrolipoamide dehydrogenase, putative 510 aa 43.0% 511 aa Compounds References
Trypanosoma cruzi trypanothione reductase, putative 492 aa 21.8% 499 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tbg972.4.5240 (Trypanosoma brucei gambiense), dihydrolipoamide dehydrogenase, putative,acetoin dehydrogenase e3 component, putative
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