Detailed view for TcCLB.510769.90

Basic information

TDR Targets ID: 54362
Trypanosoma cruzi, 60S ribosomal protein L32, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 11.8382 | Length (AA): 132 | MW (Da): 15439 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01655   Ribosomal protein L32

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 123 1vq8 (Y) 125 231 34.00 0 1 1.19 0.56
2 124 1vq8 (Y) 114 232 33.00 0 1 1.29922 0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_126965)

Species Accession Gene Product
Arabidopsis thaliana AT5G46430   60S ribosomal protein L32-2
Arabidopsis thaliana AT4G18100   60S ribosomal protein L32-1
Babesia bovis BBOV_III002830   60S ribosomal protein L32, putative
Brugia malayi Bm1_28495   60S ribosomal protein L32 (RP49)
Caenorhabditis elegans CELE_T24B8.1   Protein RPL-32, isoform B
Cryptosporidium hominis Chro.60387   ribosomal protein L32
Cryptosporidium parvum cgd6_3340   60S ribosomal protein L32
Dictyostelium discoideum DDB_G0271976   S60 ribosomal protein L32
Drosophila melanogaster Dmel_CG7939   Ribosomal protein L32
Entamoeba histolytica EHI_044770   60S ribosomal protein L32, putative
Entamoeba histolytica EHI_188940   60S ribosomal protein L32, putative
Entamoeba histolytica EHI_092430   60S ribosomal protein L32, putative
Giardia lamblia GL50803_14049   Ribosomal protein L32
Homo sapiens ENSG00000144713   ribosomal protein L32
Leishmania braziliensis LbrM.34.1960   60S ribosomal protein L32
Leishmania braziliensis LbrM.21.2030   60S ribosomal protein L32
Leishmania donovani LdBPK_212090.1   60S ribosomal protein L32
Leishmania donovani LdBPK_352040.1   60S ribosomal protein L32
Leishmania infantum LinJ.21.2090   60S ribosomal protein L32
Leishmania infantum LinJ.35.2040   60S ribosomal protein L32
Leishmania major LmjF.21.1720   60S ribosomal protein L32
Leishmania mexicana LmxM.34.2050   60S ribosomal protein L32
Leishmania mexicana LmxM.21.1720   60S ribosomal protein L32
Loa Loa (eye worm) LOAG_07050   60S ribosomal protein L32
Mus musculus 102642864   60S ribosomal protein L32-like
Mus musculus 102642959   60S ribosomal protein L32-like
Mus musculus 621697   predicted gene 6251
Mus musculus ENSMUSG00000057841   ribosomal protein L32
Neospora caninum NCLIV_038780   60S ribosomal protein L32, related
Oryza sativa 4347487   Os09g0501700
Oryza sativa 4347488   Os09g0502000
Oryza sativa 4346086   Os08g0524600
Oryza sativa 9268508   Os09g0501100
Plasmodium berghei PBANKA_0417500   60S ribosomal protein L32, putative
Plasmodium falciparum PF3D7_0903900   60S ribosomal protein L32
Plasmodium knowlesi PKNH_0701600   60S ribosomal protein L32, putative
Plasmodium vivax PVX_098640   60S ribosomal protein L32, putative
Plasmodium yoelii PY01152   Ribosomal protein L32
Saccharomyces cerevisiae YBL092W   ribosomal 60S subunit protein L32
Trypanosoma brucei gambiense Tbg972.10.180   60S ribosomal protein L32, putative
Trypanosoma brucei gambiense Tbg972.9.9570   60S ribosomal protein L32
Trypanosoma brucei Tb927.10.270   60S ribosomal protein L32, putative
Trypanosoma brucei Tb927.9.15420   60S ribosomal protein L32
Trypanosoma congolense TcIL3000_10_130   60S ribosomal protein L32
Trypanosoma cruzi TcCLB.510769.90   60S ribosomal protein L32, putative
Trypanosoma cruzi TcCLB.504069.30   60S ribosomal protein L32, putative
Trypanosoma cruzi TcCLB.511145.20   60S ribosomal protein L32, putative
Trypanosoma cruzi TcCLB.506241.100   60S ribosomal protein L32, putative
Toxoplasma gondii TGME49_267400   ribosomal protein RPL32
Theileria parva TP04_0094   60S ribosomal protein L32, putative
Trichomonas vaginalis TVAG_265940   60S ribosomal protein L32 and DNA-directed RNA polymerase II, subunit N, putative
Trichomonas vaginalis TVAG_313290   60S ribosomal protein L32, putative
Trichomonas vaginalis TVAG_265950   60S ribosomal protein L32, putative
Trichomonas vaginalis TVAG_424270   60S ribosomal protein L32, putative
Trichomonas vaginalis TVAG_265930   60S ribosomal protein L32 and DNA-directed RNA polymerase II, subunit N, putative
Trichomonas vaginalis TVAG_470330   60S ribosomal protein L32 and DNA-directed RNA polymerase II, subunit N, putative

Essentiality

TcCLB.510769.90 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.244.2590 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.244.2590 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.244.2590 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.244.2590 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.270 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.270 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T24B8.1 Caenorhabditis elegans larval arrest wormbase
CELE_T24B8.1 Caenorhabditis elegans slow growth wormbase
YBL092W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0417500 Plasmodium berghei Essential plasmo
TGME49_267400 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.510769.90 (Trypanosoma cruzi), 60S ribosomal protein L32, putative
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