Detailed view for TcCLB.511303.90

Basic information

TDR Targets ID: 54503
Trypanosoma cruzi, RNA cytidine acetyltransferase
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.4604 | Length (AA): 1043 | MW (Da): 114756 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05127   Helicase
PF08351   Domain of unknown function (DUF1726)
PF13718   GNAT acetyltransferase 2
PF13725   Possible tRNA binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0034470   GO:ncRNA processing  

GO:0016072   rRNA metabolic process  
GO:0008080   N-acetyltransferase activity  
GO:0005524   ATP binding  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
28 915 2zpa (A) 6 666 22.00 0 1 0.68059 1.19
294 458 4znk (A) 22 195 20.00 0 0.86 0.284497 0.15
307 775 2zpa (A) 197 533 34.00 0 1 0.299864 1.47
738 1026 1ofi (B) 27 440 30.00 0.41 0.96 0.284285 1.16
920 1034 4uos (A) 46 159 14.00 0.012 0.02 0.307459 -1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127380)

Species Accession Gene Product
Arabidopsis thaliana AT3G57940   hypothetical protein
Arabidopsis thaliana AT1G10490   hypothetical protein
Babesia bovis BBOV_III004380   ATPase domain containing protein
Brugia malayi Bm1_49950   Hypothetical UPF0202 protein F55A12.8 in chromosome I, putative
Candida albicans CaO19.8143   similar to S. cerevisiae YNL132W
Candida albicans CaO19.512   similar to S. cerevisiae YNL132W
Caenorhabditis elegans CELE_F55A12.8   Protein NATH-10
Cryptosporidium hominis Chro.80234   hypothetical protein
Cryptosporidium parvum cgd8_2000   KRE33p like superfamily I ATPAse fused to an acetylase
Dictyostelium discoideum DDB_G0268868   hypothetical protein
Drosophila melanogaster Dmel_CG1994   lethal (1) G0020
Escherichia coli b2474   elongator methionine tRNA (ac4C34) acetyltransferase
Echinococcus granulosus EgrG_000220800   N acetyltransferase 10
Entamoeba histolytica EHI_033750   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000220800   N acetyltransferase 10
Giardia lamblia GL50803_14277   N-acetyltransferase-like protein
Homo sapiens ENSG00000135372   N-acetyltransferase 10 (GCN5-related)
Leishmania braziliensis LbrM.17.1410   hypothetical protein, conserved
Leishmania donovani LdBPK_171350.1   RNA cytidine acetyltransferase
Leishmania infantum LinJ.17.1350   hypothetical protein, conserved
Leishmania major LmjF.17.1250   hypothetical protein, conserved
Leishmania mexicana LmxM.17.1250   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00945   hypothetical protein
Mus musculus 102642785   UPF0202 protein KRE33-like
Mus musculus ENSMUSG00000027185   N-acetyltransferase 10
Neospora caninum NCLIV_061670   Predicted P-loop ATPase fused to an acetyltransferase (ISS), related
Oryza sativa 4351627   Os12g0170700
Plasmodium berghei PBANKA_0504900   histone acetyltransferase, putative
Plasmodium falciparum PF3D7_1020700   histone acetyltransferase, putative
Plasmodium knowlesi PKNH_0604900   histone acetyltransferase, putative
Plasmodium vivax PVX_001895   histone acetyltransferase, putative
Plasmodium yoelii PY04574   hypothetical protein
Saccharomyces cerevisiae YNL132W   Kre33p
Schistosoma japonicum Sjp_0019200   ko:K06957 NAT10, LOC466485; N-acetyltransferase 10, putative
Schistosoma mansoni Smp_144490   n-acetyltransferase-related
Schmidtea mediterranea mk4.001426.02  
Schmidtea mediterranea mk4.000073.06   UPF0202 protein
Schmidtea mediterranea mk4.000938.02  
Schmidtea mediterranea mk4.001426.00  
Schmidtea mediterranea mk4.001426.03   N-acetyltransferase 10
Trypanosoma brucei gambiense Tbg972.5.3580   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.2530   RNA cytidine acetyltransferase
Trypanosoma congolense TcIL3000_5_2450   RNA cytidine acetyltransferase
Trypanosoma congolense TcIL3000_5_2490   RNA cytidine acetyltransferase
Trypanosoma cruzi TcCLB.509177.30   RNA cytidine acetyltransferase
Trypanosoma cruzi TcCLB.511303.90   RNA cytidine acetyltransferase
Toxoplasma gondii TGME49_218610   ATPase (DUF699) protein
Theileria parva TP02_0251   hypothetical protein, conserved
Trichomonas vaginalis TVAG_398720   conserved hypothetical protein

Essentiality

TcCLB.511303.90 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2530 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.2530 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.2530 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2530 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2474 Escherichia coli non-essential goodall
CELE_F55A12.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_F55A12.8 Caenorhabditis elegans larval arrest wormbase
CELE_F55A12.8 Caenorhabditis elegans slow growth wormbase
CELE_F55A12.8 Caenorhabditis elegans sterile wormbase
YNL132W Saccharomyces cerevisiae inviable yeastgenome
TGME49_218610 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.511303.90 (Trypanosoma cruzi), RNA cytidine acetyltransferase
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