Detailed view for Tbg972.10.650

Basic information

TDR Targets ID: 546346
Trypanosoma brucei gambiense, methyltransferase, putative
Source Database / ID: 

pI: 5.7469 | Length (AA): 784 | MW (Da): 86633 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05185   PRMT5 arginine-N-methyltransferase
PF17286   PRMT5 oligomerisation domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0035246   peptidyl-arginine N-methylation  
GO:0008168   methyltransferase activity  
GO:0006479   protein amino acid methylation  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
53 782 4g56 (A) 22 633 28.00 0 1 0.957322 0.82
359 749 3ua3 (A) 359 706 35.00 0 1 0.624524 0.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128174)

Species Accession Gene Product
Arabidopsis thaliana AT4G31120   protein arginine N-methyltransferase 5
Babesia bovis BBOV_IV000750   skb1 methyltransferase family protein, putative
Brugia malayi Bm1_45965   Skb1 methyltransferase family protein
Caenorhabditis elegans CELE_C34E10.5   Protein PRMT-5
Cryptosporidium hominis Chro.70401   protein methyltransferase-related
Cryptosporidium parvum cgd7_3600   Hs17p, histone methylase
Dictyostelium discoideum DDB_G0287327   Skb1 family protein
Drosophila melanogaster Dmel_CG3730   capsuleen
Echinococcus granulosus EgrG_000795200   protein arginine N methyltransferase 5
Entamoeba histolytica EHI_158560   Skb1 methyltransferase, putative
Echinococcus multilocularis EmuJ_000795200   protein arginine N methyltransferase 5
Homo sapiens ENSG00000100462   protein arginine methyltransferase 5
Leishmania braziliensis LbrM.21.1830   arginine N-methyltransferase, type II, putative;with=GeneDB:Tb927.10.640
Leishmania donovani LdBPK_211690.1   methyltransferase-like protein
Leishmania infantum LinJ.21.1690   arginine N-methyltransferase, type II, putative;with=GeneDB:Tb927.10.640
Leishmania major LmjF.21.1450   arginine N-methyltransferase, type II, putative;with=GeneDB:Tb927.10.640
Leishmania mexicana LmxM.21.1450   methyltransferase-like protein
Loa Loa (eye worm) LOAG_08049   Skb1 methyltransferase
Mus musculus ENSMUSG00000023110   protein arginine N-methyltransferase 5
Neospora caninum NCLIV_052480   Protein arginine methyltransferase 5, related
Oryza sativa 4328251   Os02g0139200
Plasmodium berghei PBANKA_1137100   protein arginine N-methyltransferase 5, putative
Plasmodium falciparum PF3D7_1361000   protein arginine N-methyltransferase 5, putative
Plasmodium knowlesi PKNH_1110400   protein arginine N-methyltransferase 5, putative
Plasmodium vivax PVX_115010   protein arginine N-methyltransferase 5, putative
Plasmodium yoelii PY07031   protein methyltransferase-related
Saccharomyces cerevisiae YBR133C   Hsl7p
Schistosoma japonicum Sjp_0063090   ko:K00599 protein arginine N-methyltransferase 5 [EC:2.1.1.-], putative
Schistosoma mansoni Smp_171150   shk1 kinase-binding protein
Schmidtea mediterranea mk4.002031.05   Protein arginine methyltransferase 5-like protein
Trypanosoma brucei gambiense Tbg972.10.650   methyltransferase, putative
Trypanosoma brucei Tb927.10.640   arginine N-methyltransferase, type II
Trypanosoma congolense TcIL3000_10_500   methyltransferase, putative
Trypanosoma cruzi TcCLB.506853.30   arginine N-methyltransferase, type II, putative
Toxoplasma gondii TGME49_215560   histone arginine methyltransferase PRMT5
Theileria parva TP01_0100   kinase binding protein, putative
Trichomonas vaginalis TVAG_096150   shk1 kinase-binding protein, putative
Trichomonas vaginalis TVAG_254540   shk1 kinase-binding protein, putative

Essentiality

Tbg972.10.650 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.640 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.640 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C34E10.5 Caenorhabditis elegans embryonic lethal wormbase
TGME49_215560 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein arginine methyltransferase 5 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tbg972.10.650 (Trypanosoma brucei gambiense), methyltransferase, putative
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