Detailed view for PF3D7_1243700

Basic information

TDR Targets ID: 5474
Plasmodium falciparum, ubiquitin-conjugating enzyme E2, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 3.9493 | Length (AA): 465 | MW (Da): 53968 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00179   Ubiquitin-conjugating enzyme

Gene Ontology

Mouse over links to read term descriptions.
GO:0019787   small conjugating protein ligase activity  
GO:0043687   post-translational protein modification  
GO:0051246   regulation of protein metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
97 242 1y6l (A) 0 141 24.00 0 1 0.74 -2.28
97 256 1y8x (A) 28 181 25.00 0.00000015 1 0.8 -1.72
315 416 3ult (A) 6 103 33.00 0.000000000033 1 0.773555 -2.13
91 274 4ddg (A) 3 1047 27.00 0.082 1 0.655125 -0.49
103 247 1pzv (A) 8 162 16.00 0.029 0.92 0.538318 -0.34
122 224 2e2c (A) 54 153 35.00 0.0034 0.98 0.651825 -0.52
329 433 3ult (A) 6 113 30.00 0.0000000000055 1 0.792882 -2.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, sporozoite, early ring, early schizont, late ring, late schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, early trophozoite, late trophozoite, Oocyst, Ring, Sporozoite, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, Male Gametocyte. Otto TD Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 24 hs. Otto TD
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_130909)

Species Accession Gene Product
Brugia malayi Bm1_55035   Ubiquitin-conjugating enzyme family protein
Caenorhabditis elegans CELE_Y69H2.6   Protein UBC-19
Cryptosporidium hominis Chro.20031   hypothetical protein
Cryptosporidium parvum cgd2_240   ubiquitin-conjugating enzyme
Dictyostelium discoideum DDB_G0278075   hypothetical protein
Drosophila melanogaster Dmel_CG16894   CG16894 gene product from transcript CG16894-RA
Drosophila melanogaster Dmel_CG10536   crossbronx
Echinococcus granulosus EgrG_000156800   AKT interacting protein
Echinococcus multilocularis EmuJ_000156800   AKT interacting protein
Homo sapiens ENSG00000166971   AKT interacting protein
Loa Loa (eye worm) LOAG_01487   hypothetical protein
Mus musculus ENSMUSG00000031667   thymoma viral proto-oncogene 1 interacting protein
Neospora caninum NCLIV_024020   ubiquitin-conjugating enzyme domain-containing protein, putative
Plasmodium berghei PBANKA_1457000   ubiquitin-conjugating enzyme E2, putative
Plasmodium falciparum PF3D7_1243700   ubiquitin-conjugating enzyme E2, putative
Plasmodium knowlesi PKNH_1463000   ubiquitin-conjugating enzyme E2, putative
Plasmodium vivax PVX_101085   ubiquitin-conjugating enzyme E2, putative
Plasmodium yoelii PY01211   ubiquitin-conjugating enzyme e2-17 kDa
Schmidtea mediterranea mk4.000287.01  
Toxoplasma gondii TGME49_264050   ubiquitin-conjugating enzyme subfamily protein
Trichomonas vaginalis TVAG_476010   fused toes, putative
Trichomonas vaginalis TVAG_130740   ubiquitin-conjugating enzyme E2, putative

Essentiality

PF3D7_1243700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1457000 Plasmodium berghei Dispensable plasmo
TGME49_264050 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1243700 (Plasmodium falciparum), ubiquitin-conjugating enzyme E2, putative
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