Detailed view for Tbg972.7.7280

Basic information

TDR Targets ID: 548910
Trypanosoma brucei gambiense, polo-like protein kinase,protein kinase
Source Database / ID: 

pI: 8.9748 | Length (AA): 609 | MW (Da): 68388 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00659   POLO box duplicated region

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 157 2rku (A) 167 319 53.00 0 1 0.9036 -0.57
1 159 4b6l (A) 176 330 48.00 0 1 0.870884 -0.71
1 143 2y7j (A) 144 291 43.00 0 1 0.786411 -0.98
1 220 3lij (A) 175 404 26.00 0 1 0.643048 -0.03
349 605 3p34 (A) 2 592 30.00 0 1 0.707803 -0.57
378 607 4o9w (A) 389 594 34.00 0 1 0.603268 -0.32
381 602 4rs6 (A) 486 682 28.00 0 1 0.604332 -0.61
536 577 5lhx (A) 670 713 31.00 0.25 0.83 0.417766 -0.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127396)

Species Accession Gene Product
Brugia malayi Bm1_14075   serine/threonine-protein kinase plk-2
Candida albicans CaO19.13431   protein serine/threonine kinase
Candida albicans CaO19.6010   protein serine/threonine kinase
Caenorhabditis elegans CELE_F55G1.8   Protein PLK-3
Caenorhabditis elegans CELE_Y71F9B.7   Protein PLK-2
Caenorhabditis elegans CELE_C14B9.4   Protein PLK-1, isoform B
Dictyostelium discoideum DDB_G0274503   polo family protein kinase
Drosophila melanogaster Dmel_CG12306   CG12306 gene product from transcript CG12306-RA
Echinococcus granulosus EgrG_000471700   serine:threonine protein kinase PLK1
Entamoeba histolytica EHI_008410   serine/threonine protein kinase, putative
Echinococcus multilocularis EmuJ_000471700   serine:threonine protein kinase PLK1
Giardia lamblia GL50803_104150   Kinase, PLK
Homo sapiens ENSG00000166851   polo-like kinase 1
Homo sapiens ENSG00000173846   polo-like kinase 3
Homo sapiens ENSG00000145632   polo-like kinase 2
Leishmania braziliensis LbrM.17.0690   protein kinase, putative,polo-like protein kinase, putative
Leishmania infantum LinJ.17.0770   protein kinase, putative,polo-like protein kinase, putative
Leishmania major LmjF.17.0790   protein kinase, putative,polo-like protein kinase, putative
Leishmania mexicana LmxM.17.0790   protein kinase, putative,polo-like protein kinase, putative
Loa Loa (eye worm) LOAG_00597   PLK/PLK1 protein kinase
Mus musculus ENSMUSG00000021701   polo-like kinase 2
Mus musculus ENSMUSG00000030867   polo-like kinase 1
Mus musculus ENSMUSG00000028680   polo-like kinase 3
Onchocerca volvulus OVOC6373   Serine\/threonine kinase homolog
Saccharomyces cerevisiae YMR001C   polo kinase CDC5
Schistosoma japonicum Sjp_0214990   Serine/threonine-protein kinase PLK1, putative
Schistosoma japonicum Sjp_0117190   ko:K06631 polo-like kinase 1, putative
Schistosoma mansoni Smp_009600   serine/threonine protein kinase
Schmidtea mediterranea mk4.003234.06   Probable serine/threonine-protein kinase zyg-1
Schmidtea mediterranea mk4.002811.01  
Schmidtea mediterranea mk4.007361.02   Serine/threonine kinase
Schmidtea mediterranea mk4.002305.03   Inactive serine/threonine-protein kinase PLK5
Schmidtea mediterranea mk4.001509.00   Serine/threonine kinase
Schmidtea mediterranea mk4.003052.00   Serine/threonine kinase
Trypanosoma brucei gambiense Tbg972.7.7280   polo-like protein kinase,protein kinase
Trypanosoma brucei Tb927.7.6310   polo-like protein kinase
Trypanosoma congolense TcIL3000_7_5220   protein kinase, putative
Trypanosoma cruzi TcCLB.506513.160   polo-like protein kinase, putative
Trypanosoma cruzi TcCLB.508917.10   polo-like protein kinase, putative
Trichomonas vaginalis TVAG_039910   CAMK family protein kinase
Trichomonas vaginalis TVAG_069810   CAMK family protein kinase
Trichomonas vaginalis TVAG_432460   CAMK family protein kinase
Trichomonas vaginalis TVAG_088660   CAMK family protein kinase
Trichomonas vaginalis TVAG_003370   CAMK family protein kinase
Trichomonas vaginalis TVAG_279730   CAMK family protein kinase
Trichomonas vaginalis TVAG_107500   CAMK family protein kinase
Trichomonas vaginalis TVAG_083120   CAMK family protein kinase
Trichomonas vaginalis TVAG_496190   CAMK family protein kinase

Essentiality

Tbg972.7.7280 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.6310 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.6310 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.6310 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.6310 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C14B9.4 Caenorhabditis elegans adult lethal wormbase
CELE_C14B9.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C14B9.4 Caenorhabditis elegans larval lethal wormbase
CELE_C14B9.4 Caenorhabditis elegans sterile wormbase
CELE_Y71F9B.7 Caenorhabditis elegans sterile wormbase
YMR001C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens polo-like kinase 1 Compounds References
Xenopus laevis Serine/threonine-protein kinase PLK1 Compounds References
Homo sapiens polo-like kinase 3 Compounds References
Homo sapiens polo-like kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tbg972.7.7280 (Trypanosoma brucei gambiense), polo-like protein kinase,protein kinase
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