Detailed view for TcCLB.507047.50

Basic information

TDR Targets ID: 54987
Trypanosoma cruzi, Alpha/beta hydrolase family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.4503 | Length (AA): 414 | MW (Da): 46803 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12697   Alpha/beta hydrolase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0004091   carboxylesterase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
88 401 1u2e (A) 0 283 11.00 0 0.98 0.72 -0.48
98 401 1a8q () 2 274 16.00 0 0.61 0.73 -0.9
88 214 1zi8 (A) 2 126 15.00 0 0.63 0.432363 -0.79
95 239 4zi5 (A) 2 145 26.00 0.41 1 0.697342 -0.61
330 388 3hss (A) 191 246 38.00 0.85 0.18 0.470612 0.16
335 402 3bdi (A) 142 206 25.00 0.00033 0.04 0.341851 0.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127073)

Species Accession Gene Product
Arabidopsis thaliana AT1G34340   alpha/beta-hydrolase domain-containing protein
Arabidopsis thaliana AT5G49950   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT3G50790   esterase/lipase/thioesterase family protein
Candida albicans CaO19.9446   similar to S. cerevisiae YMR210W
Candida albicans CaO19.10558   alcohol acyl transferase
Candida albicans CaO19.3040   alcohol acyl transferase
Candida albicans CaO19.1890   similar to S. cerevisiae YMR210W
Caenorhabditis elegans CELE_C44C1.5   Protein C44C1.5, isoform B
Caenorhabditis elegans CELE_Y60A3A.7   Protein Y60A3A.7
Dictyostelium discoideum DDB_G0289723   abhydrolase domain-containing protein
Drosophila melanogaster Dmel_CG8058   alpha/beta hydrolase 1
Escherichia coli b3353   UPF0017 family putative hydrolase
Echinococcus granulosus EgrG_000295600   abhydrolase domain containing 3
Echinococcus granulosus EgrG_000295900   abhydrolase domain containing protein 3
Entamoeba histolytica EHI_068640   alpha/beta hydrolase fold protein, putative
Entamoeba histolytica EHI_152050   alpha/beta hydrolase domain protein, putative
Entamoeba histolytica EHI_030780   hypothetical protein
Echinococcus multilocularis EmuJ_000295800   abhydrolase domain containing 3
Echinococcus multilocularis EmuJ_000295900   abhydrolase domain containing protein 3
Echinococcus multilocularis EmuJ_000295600   abhydrolase domain containing 3
Homo sapiens ENSG00000158201   abhydrolase domain containing 3
Homo sapiens ENSG00000143994   abhydrolase domain containing 1
Leishmania braziliensis LbrM.30.0020   hypothetical protein, conserved
Leishmania donovani LdBPK_300010.1   Alpha/beta hydrolase family, putative
Leishmania infantum LinJ.30.0010   hypothetical protein, conserved
Leishmania major LmjF.30.0010   hypothetical protein, conserved
Leishmania mexicana LmxM.29.0010   hypothetical protein, conserved
Mus musculus ENSMUSG00000002475   abhydrolase domain containing 3
Mus musculus ENSMUSG00000006638   abhydrolase domain containing 1
Oryza sativa 4338886   Os05g0432600
Oryza sativa 4324939   Os01g0871100
Oryza sativa 4330156   Os02g0649400
Saccharomyces cerevisiae YMR210W   putative carboxylic ester hydrolase
Saccharomyces cerevisiae YBR177C   Eht1p
Saccharomyces cerevisiae YPL095C   Eeb1p
Schmidtea mediterranea mk4.053903.00   Alpha/beta hydrolase 1
Schmidtea mediterranea mk4.038550.01   Alpha/beta hydrolase 1
Schmidtea mediterranea mk4.012425.01   Alpha/beta hydrolase 1
Trypanosoma brucei gambiense Tbg972.5.3850   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.2750   Alpha/beta hydrolase family, putative
Trypanosoma congolense TcIL3000_0_43240   Alpha/beta hydrolase family, putative
Trypanosoma congolense TcIL3000_0_59540   Alpha/beta hydrolase family, putative
Trypanosoma cruzi TcCLB.509179.220   Alpha/beta hydrolase family, putative
Trypanosoma cruzi TcCLB.507047.50   Alpha/beta hydrolase family, putative
Trichomonas vaginalis TVAG_008710   Clan SC, family S33, methylesterase-like serine peptidase
Trichomonas vaginalis TVAG_057110   Clan SC, family S33, methylesterase-like serine peptidase
Trichomonas vaginalis TVAG_020790   Clan SC, family S33, methylesterase-like serine peptidase
Trichomonas vaginalis TVAG_251570   Clan SC, family S33, methylesterase-like serine peptidase
Trichomonas vaginalis TVAG_120390   Clan SC, family S33, methylesterase-like serine peptidase

Essentiality

TcCLB.507047.50 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2750 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.2750 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.2750 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2750 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3353 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.507047.50 (Trypanosoma cruzi), Alpha/beta hydrolase family, putative
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