Detailed view for PF3D7_1247500

Basic information

TDR Targets ID: 5510
Plasmodium falciparum, serine/threonine protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.1208 | Length (AA): 909 | MW (Da): 105537 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004693   cyclin-dependent protein kinase activity  
GO:0030140   trans-Golgi network transport vesicle  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
12 300 2buj (A) 8 298 24.00 0 1 0.71 -1.52
16 382 2b9h (A) 6 350 24.00 0 1 0.65 -0.56
489 600 1ktm (A) 921 1036 13.00 0.000000012 0.05 0.22 -0.24
33 516 3q5i (A) 64 509 31.00 0.000000000017 1 0.567553 0.89
258 758 4h5y (A) 60 526 24.00 0.28 0.7 0.635255 0.7
622 800 4u5a (C) 48 239 21.00 0.45 0.02 0.34712 -0.14
643 784 4ux7 (A) 81 218 32.00 0.9 0.18 0.206016 1
12 296 4w9w (A) 39 319 34.00 0 1 0.807229 -0.9
25 548 4e3c (A) 16 614 18.00 0 1 0.604304 0.81
27 232 4krd (A) 11 199 39.00 0.0000000098 1 0.47666 0.21
68 282 1phk (A) 72 275 31.00 0.00000071 1 0.525864 -0.28
71 527 4ci6 (B) 179 642 17.00 0.0002 1 0.566341 0.43
142 245 3a99 (A) 157 245 37.00 0.15 0.22 0.136014 1.18
346 539 4f61 (I) 16 229 21.00 0.19 0.01 0.354055 -0.39

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, gametocyte, late schizont, Female Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, early schizont, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Oocyst, Ring. Zanghi G
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127425)

Species Accession Gene Product
Arabidopsis thaliana AT2G32850   protein kinase superfamily protein
Brugia malayi Bm1_37065   Protein kinase domain containing protein
Candida albicans CaO19.5357   Ark-family kinase-like protein
Candida albicans CaO19.2605   possible serine/threonine kinase
Candida albicans CaO19.10136   possible serine/threonine kinase
Candida albicans CaO19.12817   Ark-family kinase-like protein
Caenorhabditis elegans CELE_F35G12.3   Protein SEL-5, isoform B
Dictyostelium discoideum DDB_G0276461   NAK family protein kinase
Dictyostelium discoideum DDB_G0280111   NAK family protein kinase
Drosophila melanogaster Dmel_CG10637   Numb-associated kinase
Echinococcus granulosus EgrG_000165700   AP2 associated protein kinase 1
Entamoeba histolytica EHI_103290   protein kinase, putative
Entamoeba histolytica EHI_127410   protein kinase domain containing protein
Echinococcus multilocularis EmuJ_000165700   AP2 associated protein kinase 1
Giardia lamblia GL50803_12223   Kinase, NAK
Homo sapiens ENSG00000138756   BMP2 inducible kinase
Homo sapiens ENSG00000115977   AP2 associated kinase 1
Leishmania braziliensis LbrM.20.0110   protein kinase, putative
Leishmania donovani LdBPK_340030.1   protein kinase, putative
Leishmania infantum LinJ.34.0030   protein kinase, putative
Leishmania major LmjF.34.0030   protein kinase, putative
Leishmania mexicana LmxM.33.0030   protein kinase, putative
Loa Loa (eye worm) LOAG_05227   NAK/BIKE protein kinase
Mus musculus ensembl-mmu:ENSMUSG00000034663   BMP2 inducible kinase
Mus musculus 102637099   uncharacterized LOC102637099
Mus musculus ENSMUSG00000057230   AP2 associated kinase 1
Neospora caninum NCLIV_050490   hypothetical protein
Oryza sativa 4346601   Os09g0279100
Plasmodium berghei PBANKA_1460500   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_1247500   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_1467200   serine/threonine protein kinase, putative
Plasmodium vivax PVX_101265   cyclin g-associated kinase, putative
Plasmodium yoelii PY06849   hypothetical protein
Saccharomyces cerevisiae YBR059C   Akl1p
Saccharomyces cerevisiae YIL095W   Prk1p
Saccharomyces cerevisiae YNL020C   Ark1p
Schistosoma japonicum Sjp_0086670   ko:K08854 BMP2 inducible kinase, putative
Schistosoma japonicum Sjp_0107280   AP2-associated protein kinase 1, putative
Schistosoma mansoni Smp_176750   serine/threonine protein kinase
Schmidtea mediterranea mk4.015925.00  
Schmidtea mediterranea mk4.000160.18  
Schmidtea mediterranea mk4.006232.02  
Schmidtea mediterranea mk4.006232.01  
Trypanosoma cruzi TcCLB.506953.30   serine/threonine-protein kinase NAK, putative
Trypanosoma cruzi TcCLB.509287.20   serine/threonine-protein kinase NAK, putative
Toxoplasma gondii TGME49_236240   Tyrosine kinase-like (TKL) protein
Trichomonas vaginalis TVAG_144060   CAMK family protein kinase
Trichomonas vaginalis TVAG_475520   CAMK family protein kinase

Essentiality

PF3D7_1247500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_F35G12.3 Caenorhabditis elegans embryonic lethal wormbase
YNL020C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1460500 Plasmodium berghei Slow plasmo
TGME49_236240 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens BMP2 inducible kinase Compounds References
Homo sapiens AP2 associated kinase 1 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 25.3% 178 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0063 1 1
0.0056 1 0.5
0.0088 0.4477 0.5
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0032 0.5 0.5
0.0059 1 1
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0023 0.5 0.5
0.0011 1 0.5
0.0062 0.6935 0
0.0029 0.5 0.5
0.0093 0.8828 0
0.0037 1 0.5
0.0018 0.5 0.5
0.0061 0.6883 1
0.0039 0.5 0.5
0.0063 0.7244 0.7244
0.0007 0.5 0.5
0.0092 1 0.5
0.0003 0.5 0.5
0.0026 0.5 0.5
0.0091 1 0.5
0.0027 1 0.5
0.0012 0.5 0.5
0.0069 0.3067 1
0.0032 0.5 0.5
0.0081 1 0.5
0.0066 0.3101 0
0.0059 1 1
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0067 0.5 0.5
0.0039 0.5 0.5
0.0042 0.5 0.5
0.0022 0.5 0.5
0.0036 0.5 0.5
0.0008 0.5 0.5
0.0033 1 1
0.0039 0.9485 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

56 literature references were collected for this gene.

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Gene identifier PF3D7_1247500 (Plasmodium falciparum), serine/threonine protein kinase, putative
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