Detailed view for Tb927.2.6000

Basic information

TDR Targets ID: 55273
Trypanosoma brucei, glycosylphosphatidylinositol-specific phospholipase C
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9104 | Length (AA): 358 | MW (Da): 40663 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00388   Phosphatidylinositol-specific phospholipase C, X domain
PF03490   Variant-surface-glycoprotein phospholipase C

Gene Ontology

Mouse over links to read term descriptions.
GO:0006507   GPI anchor release  
GO:0005886   plasma membrane  
GO:0047396   glycosylphosphatidylinositol diacylglycerol-lyase activity  
GO:0008081   phosphoric diester hydrolase activity  
GO:0004629   phospholipase C activity  
GO:0007242   intracellular signaling cascade  
GO:0006650   glycerophospholipid metabolic process  
GO:0006629   lipid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130000)

Species Accession Gene Product
Brugia malayi Bm1_57135   Phosphatidylinositol-specific phospholipase C, X domain containing protein
Brugia malayi Bm1_36730   MGC53544 protein
Caenorhabditis elegans CELE_F38E9.1   Protein F38E9.1
Dictyostelium discoideum DDB_G0267488   hypothetical protein
Dictyostelium discoideum DDB_G0293730   TIM beta/alpha-barrel domain-containing protein
Dictyostelium discoideum DDB_G0269228   phosphatidylinositol-specific phospholipase C X domain-containing protein
Drosophila melanogaster Dmel_CG10747   CG10747 gene product from transcript CG10747-RB
Echinococcus granulosus EgrG_000999500   PLC phosphodiesterase TIM beta alpha barrel domain
Echinococcus granulosus EgrG_001071600   Phospholipase C
Entamoeba histolytica EHI_012410   hypothetical protein
Entamoeba histolytica EHI_049280   hypothetical protein
Entamoeba histolytica EHI_103490   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000999500   PLC phosphodiesterase, TIM beta alpha barrel domain
Echinococcus multilocularis EmuJ_001071600   Phospholipase C
Homo sapiens ENSG00000240891   phosphatidylinositol-specific phospholipase C, X domain containing 2
Homo sapiens ENSG00000182836   phosphatidylinositol-specific phospholipase C, X domain containing 3
Loa Loa (eye worm) LOAG_14342   phosphatidylinositol-specific phospholipase C
Loa Loa (eye worm) LOAG_05941   hypothetical protein
Mus musculus ENSMUSG00000049148   phosphatidylinositol-specific phospholipase C, X domain containing 3
Mus musculus ENSMUSG00000087141   phosphatidylinositol-specific phospholipase C, X domain containing 2
Onchocerca volvulus OVOC9286  
Schistosoma japonicum Sjp_0026320   ko:K01114 phospholipase C [EC3.1.4.3], putative
Schistosoma mansoni Smp_018360   hypothetical protein
Schmidtea mediterranea mk4.029690.00  
Schmidtea mediterranea mk4.000258.07  
Schmidtea mediterranea mk4.004696.00  
Schmidtea mediterranea mk4.001510.02  
Schmidtea mediterranea mk4.000819.02  
Schmidtea mediterranea mk4.001510.03  
Trypanosoma brucei gambiense Tbg.972.2.4210   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma brucei Tb927.2.6000   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma congolense TcIL3000_2_1660   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma cruzi TcCLB.508765.30   VSG lipase
Trypanosoma cruzi TcCLB.506821.30   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma cruzi TcCLB.508807.5   VSG lipase
Trypanosoma cruzi TcCLB.508233.50   vsglipase, putative
Trypanosoma cruzi TcCLB.506617.70   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma cruzi TcCLB.507041.120   variant-surface-glycoprotein phospholipase C, putative

Essentiality

Tb927.2.6000 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.6000 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.6000 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.6000 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.6000 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from bioassay (ECO:0000094) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: Drug: glucosaminylinsitol derivatives. . References: 7852313
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) epimastigote (BTO:0000409) inferred from bioassay (ECO:0000094) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: Drug: glucosaminylinsitol derivatives. . References: 7852313
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: Drug: glucosaminylinsitol derivatives. . References: 7852313
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 8 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.2.6000 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 4 )

Bibliographic References

3 literature references were collected for this gene.

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Gene identifier Tb927.2.6000 (Trypanosoma brucei), glycosylphosphatidylinositol-specific phospholipase C
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