Detailed view for TcIL3000_8_3050

Basic information

TDR Targets ID: 555239
Trypanosoma congolense, metallo-peptidase, Clan MF, Family M17, putative
Source Database / ID: 

pI: 7.7049 | Length (AA): 596 | MW (Da): 62880 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00883   Cytosol aminopeptidase family, catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005622   intracellular  
GO:0030145   manganese ion binding  
GO:0008235   metalloexopeptidase activity  
GO:0004177   aminopeptidase activity  
GO:0019538   protein metabolic process  
GO:0006508   proteolysis  

Metabolic Pathways

Glutathione metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 549 4ksi (A) 60 571 27.00 0 1 1.17194 -0.08
28 550 3jru (B) 1 489 32.00 0 1 1.22462 -0.4
85 143 4kl0 (A) 147 212 37.00 0.94 0.11 0.370593 -0.1
217 549 5nth (A) 204 539 33.00 0 1 0.989825 -0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127293)

Species Accession Gene Product
Arabidopsis thaliana AT4G30920   leucine aminopeptidase 2
Arabidopsis thaliana AT4G30910   cytosol aminopeptidase family protein
Arabidopsis thaliana AT2G24200   aminopeptidase family protein
Babesia bovis BBOV_II004450   leucine aminopeptidase, putative
Cryptosporidium hominis Chro.50116   leucine aminopeptidase
Cryptosporidium parvum cgd5_2600   putative leucine aminopeptidase; of possible plant or bacterial origin
Chlamydia trachomatis CT_045   cytosol aminopeptidase
Dictyostelium discoideum DDB_G0279793   leucine aminopeptidase
Drosophila melanogaster Dmel_CG13340   Sperm-Leucylaminopeptidase 7
Drosophila melanogaster Dmel_CG4439   Sperm-Leucylaminopeptidase 8
Drosophila melanogaster Dmel_CG32351   Sperm-Leucylaminopeptidase 2
Drosophila melanogaster Dmel_CG6372   Sperm-Leucylaminopeptidase 1
Drosophila melanogaster Dmel_CG18369   Sperm-Leucylaminopeptidase 5
Drosophila melanogaster Dmel_CG32064   Sperm-Leucylaminopeptidase 4
Drosophila melanogaster Dmel_CG32063   Sperm-Leucylaminopeptidase 3
Drosophila melanogaster Dmel_CG4750   CG4750 gene product from transcript CG4750-RC
Escherichia coli b4260   multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site-specific recombination factor
Echinococcus granulosus EgrG_001133600   leucine aminopeptidase protein
Echinococcus multilocularis EmuJ_001133600   leucine aminopeptidase protein
Homo sapiens ENSG00000002549   leucine aminopeptidase 3
Leishmania braziliensis LbrM.23.1030   cytosolic leucyl aminopeptidase,metallo-peptidase, Clan MF, Family M17
Leishmania donovani LdBPK_231120.1   cytosolic leucyl aminopeptidase
Leishmania infantum LinJ.23.1120   cytosolic leucyl aminopeptidase,metallo-peptidase, Clan MF, Family M17
Leishmania major LmjF.23.0950   cytosolic leucyl aminopeptidase,metallo-peptidase, Clan MF, Family M17
Leishmania mexicana LmxM.23.0950   cytosolic leucyl aminopeptidase,metallo-peptidase, Clan MF, Family M17
Mycobacterium leprae ML0864c   Probable cytosol aminopeptidase PepB
Mus musculus ENSMUSG00000039682   leucine aminopeptidase 3
Mycobacterium tuberculosis Rv2213   Probable aminopeptidase PepB
Mycobacterium ulcerans MUL_3573   leucyl aminopeptidase
Neospora caninum NCLIV_042660   Probable cytosol aminopeptidase, related
Oryza sativa 4352123   Os12g0434400
Oryza sativa 4330998   Os02g0794700
Plasmodium berghei PBANKA_1309900   M17 leucyl aminopeptidase, putative
Plasmodium falciparum PF3D7_1446200   M17 leucyl aminopeptidase
Plasmodium knowlesi PKNH_1236000   M17 leucyl aminopeptidase, putative
Plasmodium vivax PVX_118180   M17 leucyl aminopeptidase, putative
Plasmodium yoelii PY01898   putative leucine aminopeptidase
Schmidtea mediterranea mk4.044959.00  
Trypanosoma brucei gambiense Tbg972.8.2770   cytosolic leucyl aminopeptidase, putative,metallo-peptidase, Clan MF, Family M17, putative
Trypanosoma brucei Tb927.8.3060   metallo-peptidase, Clan MF, Family M17
Trypanosoma congolense TcIL3000_8_3050   metallo-peptidase, Clan MF, Family M17, putative
Trypanosoma cruzi TcCLB.509859.40   metallo-peptidase, Clan MF, Family M17, putative
Trypanosoma cruzi TcCLB.510515.60   cytosolic leucyl aminopeptidase, putative
Toxoplasma gondii TGME49_290670   leucyl aminopeptidase LAP
Theileria parva TP04_0561   leucine aminopeptidase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0630   leucyl aminopeptidase

Essentiality

TcIL3000_8_3050 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3060 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.3060 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3060 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.3060 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b4260 Escherichia coli non-essential goodall
PBANKA_1309900 Plasmodium berghei Slow plasmo
TGME49_290670 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Sus scrofa Cytosol aminopeptidase Compounds References
Plasmodium falciparum M17 leucyl aminopeptidase Compounds References
Leishmania major cytosolic leucyl aminopeptidase,metallo-peptidase, Clan MF, Family M17 Compounds References
Bos taurus Cytosol aminopeptidase Compounds References
Homo sapiens leucine aminopeptidase 3 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcIL3000_8_3050 (Trypanosoma congolense), metallo-peptidase, Clan MF, Family M17, putative
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