Detailed view for Rv3045

Basic information

TDR Targets ID: 5640
Mycobacterium tuberculosis, Probable NADP-dependent alcohol dehydrogenase AdhC
Source Database / ID:  Tuberculist 

pI: 4.933 | Length (AA): 346 | MW (Da): 37075 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00107   Zinc-binding dehydrogenase
PF08240   Alcohol dehydrogenase GroES-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0016491   oxidoreductase activity  
GO:0008270   zinc ion binding  
GO:0008152   metabolic process  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 344 3s2e (A) 4 341 34.00 0 1 1.44462 -0.57
2 344 5vkt (A) 8 353 45.00 0 1 1.62743 -0.94
4 346 1uuf (A) 3 347 51.00 0 1 1.63543 -0.62
6 344 1yqd (A) 15 354 47.00 0 1 1.61167 -0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_126661)

Species Accession Gene Product
Arabidopsis thaliana AT4G34230   cinnamyl alcohol dehydrogenase 5
Arabidopsis thaliana AT2G21730   cinnamyl alcohol dehydrogenase 2
Arabidopsis thaliana AT4G37980   cinnamyl alcohol dehydrogenase 7
Arabidopsis thaliana AT4G39330   putative cinnamyl alcohol dehydrogenase 9
Arabidopsis thaliana AT3G19450   cinnamyl alcohol dehydrogenase 4
Arabidopsis thaliana AT4G37970   cinnamyl-alcohol dehydrogenase 6
Arabidopsis thaliana AT2G21890   cinnamyl alcohol dehydrogenase-like protein 3
Arabidopsis thaliana AT4G37990   cinnamyl alcohol dehydrogenase 8
Candida albicans CaO19.11480   immunogenic alcohol dehydrogenase
Candida albicans CaO19.5113   alcohol dehydrogenase 2
Candida albicans CaO19.12579   alcohol dehydrogenase 2
Candida albicans CaO19.3997   immunogenic alcohol dehydrogenase
Candida albicans CaO19.12963   similar to S. cerevisiae ADH7 (YCR105W) and ADH6 (YMR318C) medium chain alcohol dehydrogenases
Candida albicans CaO19.5517   similar to S. cerevisiae ADH7 (YCR105W) and ADH6 (YMR318C) medium chain alcohol dehydrogenases
Caenorhabditis elegans CELE_K12G11.3   Protein SODH-1
Caenorhabditis elegans CELE_K12G11.4   Protein SODH-2
Caenorhabditis elegans CELE_D2063.1   Protein D2063.1
Cryptosporidium hominis Chro.50110   ENSANGP00000000281
Cryptosporidium parvum cgd5_2670   predicted mannitol dehydrogenase; zinc dependent alcohol dehydrogenase like rossmann fold
Dictyostelium discoideum DDB_G0271680   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0272628   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0273155   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0273921   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0268322   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0274085   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0271740   zinc-containing alcohol dehydrogenase
Dictyostelium discoideum DDB_G0271780   zinc-containing alcohol dehydrogenase
Escherichia coli b0325   aldehyde reductase, NADPH-dependent, Zn-containing, broad specificity
Escherichia coli b4269   aldehyde reductase, NADPH-dependent, Zn-containing, broad specificity
Escherichia coli b1478   ethanol-active dehydrogenase/acetaldehyde-active reductase
Leishmania braziliensis LbrM.23.0400   NADP-dependent alcohol dehydrogenase, putative
Leishmania donovani LdBPK_230410.1   NADP-dependent alcohol dehydrogenase, putative
Leishmania infantum LinJ.23.0410   NADP-dependent alcohol dehydrogenase, putative
Leishmania major LmjF.23.0360   NADP-dependent alcohol dehydrogenase, putative
Leishmania mexicana LmxM.23.0360   NADP-dependent alcohol dehydrogenase, putative
Mycobacterium leprae ML2053c   Probable alcohol dehydrogenase AdhA
Mycobacterium leprae ML1730   PROBABLE NADP-DEPENDENT ALCOHOL DEHYDROGENASE ADHC
Mycobacterium tuberculosis Rv1862   Probable alcohol dehydrogenase AdhA
Mycobacterium tuberculosis Rv3045   Probable NADP-dependent alcohol dehydrogenase AdhC
Mycobacterium ulcerans MUL_2965   NADP-dependent alcohol dehydrogenase AdhC
Mycobacterium ulcerans MUL_3015   alcohol dehydrogenase AdhA
Mycobacterium ulcerans MUL_0495   Zn-dependent alcohol dehydrogenase, AdhX
Mycobacterium ulcerans MUL_1885   NADP-dependent alcohol dehydrogenase Adh
Oryza sativa 9271744   Os08g0270433
Oryza sativa 4328552   Os02g0187800
Oryza sativa 4348690   Os10g0430200
Oryza sativa 9269515   Os09g0400300
Oryza sativa 9267509   Os08g0270466
Oryza sativa 4335223   Os04g0229100
Oryza sativa 4346989   Os09g0400000
Oryza sativa 4336968   Os04g0612700
Oryza sativa 4346988   Os09g0399800
Oryza sativa 9266669   Os03g0223200
Oryza sativa 4346993   Os09g0400400
Saccharomyces cerevisiae YMR318C   Adh6p
Saccharomyces cerevisiae YMR083W   alcohol dehydrogenase ADH3
Saccharomyces cerevisiae YMR303C   alcohol dehydrogenase ADH2
Saccharomyces cerevisiae YOL086C   alcohol dehydrogenase ADH1
Saccharomyces cerevisiae YBR145W   alcohol dehydrogenase ADH5
Saccharomyces cerevisiae YCR105W   Adh7p
Trypanosoma cruzi TcCLB.504339.19   NADP-dependent alcohol hydrogenase, putative
Trypanosoma cruzi TcCLB.508677.80   NADP-dependent alcohol hydrogenase, putative
Trypanosoma cruzi TcCLB.504425.60   NADP-dependent alcohol hydrogenase, putative
Trypanosoma cruzi TcCLB.509331.210   NADP-dependent alcohol hydrogenase, putative

Essentiality

Rv3045 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1892 Mycobacterium tuberculosis non-essential nmpdr
mtu3098 this record Mycobacterium tuberculosis non-essential nmpdr
b0325 Escherichia coli non-essential goodall
b1478 Escherichia coli non-essential goodall
b4269 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Alcohol Dehydrogenase, NADP+ Dependent (1.1.1.2 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3045 (Mycobacterium tuberculosis), Probable NADP-dependent alcohol dehydrogenase AdhC
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