Detailed view for Rv3708c

Basic information

TDR Targets ID: 5690
Mycobacterium tuberculosis, Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-sem

Source Database / ID:  Tuberculist 

pI: 4.6439 | Length (AA): 345 | MW (Da): 36230 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01118   Semialdehyde dehydrogenase, NAD binding domain
PF02774   Semialdehyde dehydrogenase, dimerisation domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016620   oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor  
GO:0005737   cytoplasm  
GO:0005488   binding  
GO:0004073   aspartate-semialdehyde dehydrogenase activity  
GO:0003824   catalytic activity  
GO:0051287   NAD binding  
GO:0050661   NADP binding  
GO:0046983   protein dimerization activity  
GO:0055114   oxidation reduction  
GO:0009097   isoleucine biosynthetic process  
GO:0009089   lysine biosynthetic process via diaminopimelate  
GO:0009088   threonine biosynthetic process  
GO:0009086   methionine biosynthetic process  
GO:0008652   amino acid biosynthetic process  
GO:0008152   metabolic process  
GO:0006520   amino acid metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 343 3tz6 (A) 2 343 99.99 0 1 2.1949 -1.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

  • 3LLG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 3TZ6:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 3VOS:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date


Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.


Ortholog group members (OG5_128664)

Species Accession Gene Product
Arabidopsis thaliana AT1G14810   aspartate semialdehyde dehydrogenase
Candida albicans CaO19.1559   aspartic-semi-aldehyde dehydrogenase
Candida albicans CaO19.9132   aspartic-semi-aldehyde dehydrogenase
Chlamydia trachomatis CT_363   aspartate semialdehyde dehydrogenase
Escherichia coli b2319   putative semialdehyde dehydrogenase
Mycobacterium tuberculosis Rv3708c   Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-sem
Mycobacterium ulcerans MUL_4296   aspartate-semialdehyde dehydrogenase
Neospora caninum NCLIV_020270   Aspartate-semialdehyde dehydrogenase (EC, related
Oryza sativa 4334188   Os03g0760700
Saccharomyces cerevisiae YDR158W   aspartate-semialdehyde dehydrogenase
Toxoplasma gondii TGME49_205420   aspartate-semialdehyde dehydrogenase
Wolbachia endosymbiont of Brugia malayi Wbm0042   aspartate-semialdehyde dehydrogenase


Rv3708c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3772 this record Mycobacterium tuberculosis essential nmpdr
b2319 Escherichia coli non-essential goodall
TGME49_205420 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site,, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacterpylori) Aspartate-semialdehyde dehydrogenase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model


Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Mycobacterium tuberculosis ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Rv3708c cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Mycobacterium tuberculosis ( 1 )

Bibliographic References

8 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3708c (Mycobacterium tuberculosis), Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-sem
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